Inhibition of cell-intrinsic NF-κB activity and metastatic abilities of breast cancer by aloe-emodin and emodic-acid isolated from Asphodelus microcarpus

Abdellatef, Amira A.; Fathy, Moustafa; Mohammed, Abd El-Salam I.; Bakr, Marwa S. Abu; Ahmed, Amal H.; Abbass, Hatem S.; El‐Desoky, Ahmed H.; Morita, Hiroyuki; Nikaido, Toshio; Hayakawa, Yoshihiro

doi:10.1007/s11418-021-01526-w

Cited by 38 publications

(18 citation statements)

References 44 publications

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“…Screening for novel applications for natural [19,33] or synthetic [34][35][36] candidates, modulating various pathways, became a remarkable approach [37,38]. In the current study, we showed that IRI increased the lipid peroxidation by-product MDA and increased Scr and BUN levels, as previously reported [39][40][41].…”

Section: Discussionsupporting

confidence: 87%

“…Macrophages are activated by extracellular signaling pathways, including proinflammatory factors such as IL-1β, TNF-α, and IL-6, which in turn activate several intracellular signaling pathways, including the mammalian mitogen-activated protein kinase (MAPK) JNK/p38/ERK and the nuclear factor-kappaB (NF-κB) pathways [16,17]. To induce the expression of pro-inflammatory genes, NF-κB is activated firstly, then translocated into the nucleus, and is involved in a variety of pathological processes and inflammatory diseases [18,19]. The inhibition of the release of pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α is crucial for the control of AKI.…”

Section: Introductionmentioning

confidence: 99%

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Pantoprazole Attenuates MAPK (ERK1/2, JNK, p38)–NF-κB and Apoptosis Signaling Pathways after Renal Ischemia/Reperfusion Injury in Rats

Fawzy

Maher

Bakkar

et al. 2021

IJMS

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Ischemia/reperfusion injury (IRI) in the kidney is the most common cause of acute renal dysfunction through different cell damage mechanisms. This study aimed to investigate, on molecular basics for the first time, the effect of pantoprazole on renal IRI in rats. Different biochemical parameters and oxidative stress markers were assessed. ELISA was used to estimate proinflammatory cytokines. qRT-PCR and western blot were used to investigate the gene and protein expression. Renal histopathological examination was also performed. IRI resulted in tissue damage, elevation of serum levels of creatinine, urea nitrogen, malondialdehyde, TNF-α, IL-6, IL-1β, up-regulation of NF-κB, JNK1/2, ERK1/2, p38, and cleaved caspase-3 proteins. Furthermore, it up-regulated the expression of the Bax gene and down-regulated the expression of the Bcl-2 gene. Treatment of the injured rats with pantoprazole, either single dose or multiple doses, significantly alleviated IRI-induced biochemical and histopathological changes, attenuated the levels of proinflammatory cytokines, down-regulated the expression of NF-κB, JNK1/2, ERK1/2, p38, and cleaved caspase-3 proteins, and the Bax gene, and up-regulated Bcl-2 gene expression. Moreover, treatment with pantoprazole multiple doses has an ameliorative effect that is greater than pantoprazole single-dose. In conclusion, pantoprazole diminished renal IRI via suppression of apoptosis, attenuation of the pro-inflammatory cytokines’ levels, and inhibition of the intracellular signaling pathway MAPK (ERK1/2, JNK, p38)–NF-κB.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Pantoprazole Attenuates MAPK (ERK1/2, JNK, p38)–NF-κB and Apoptosis Signaling Pathways after Renal Ischemia/Reperfusion Injury in Rats

Fawzy

Maher

Bakkar

et al. 2021

IJMS

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“…Natural agents exhibited many in vitro [31,32] and in vivo [33,34] pharmacological actions in various disorders like carcinogenesis, [35] fibrosis, [36,37] angiogenesis, [38] and immunomodulation [39]. Several natural products have been examined for their hypoglycaemic activity against the insulin resistant model of HepG2 cells.…”

Section: Discussionmentioning

confidence: 99%

Carpachromene Ameliorates Insulin Resistance in HepG2 Cells via Modulating IR/IRS1/PI3k/Akt/GSK3/FoxO1 Pathway

et al. 2021

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Insulin resistance contributes to several disorders including type 2 diabetes and cardiovascular diseases. Carpachromene is a natural active compound that inhibits α-glucosidase enzyme. The aim of the present study is to investigate the potential activity of carpachromene on glucose consumption, metabolism and insulin signalling in a HepG2 cells insulin resistant model. A HepG2 insulin resistant cell model (HepG2/IRM) was established. Cell viability assay of HepG2/IRM cells was performed after carpachromene/metformin treatment. Glucose concentration and glycogen content were determined. Western blot analysis of insulin receptor, IRS1, IRS2, PI3k, Akt, GSK3, FoxO1 proteins after carpachromene treatment was performed. Phosphoenolpyruvate carboxykinase (PEPCK) and hexokinase (HK) enzymes activity was also estimated. Viability of HepG2/IRM cells was over 90% after carpachromene treatment at concentrations 6.3, 10, and 20 µg/mL. Treatment of HepG2/IRM cells with carpachromene decreased glucose concentration in a concentration- and time-dependant manner. In addition, carpachromene increased glycogen content of HepG2/IRM cells. Moreover, carpachromene treatment of HepG2/IRM cells significantly increased the expression of phosphorylated/total ratios of IR, IRS1, PI3K, Akt, GSK3, and FoxO1 proteins. Furthermore, PEPCK enzyme activity was significantly decreased, and HK enzyme activity was significantly increased after carpachromene treatment. The present study examined, for the first time, the potential antidiabetic activity of carpachromene on a biochemical and molecular basis. It increased the expression ratio of insulin receptor and IRS1 which further phosphorylated/activated PI3K/Akt pathway and phosphorylated/inhibited GSK3 and FoxO1 proteins. Our findings revealed that carpachromene showed central molecular regulation of glucose metabolism and insulin signalling via IR/IRS1/ PI3K/Akt/GSK3/FoxO1 pathway.

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“…For many years, natural products have played an essential role in the management of various disorders [ 1 , 2 , 3 , 4 , 5 , 6 ] and constitute a large part of current-day pharmaceutical products, especially in the field of antibiotic and anticancer drugs. It is important to mention that more than 60% of current anticancer drugs come from natural sources [ 7 , 8 , 9 , 10 ]. In other pathophysiological conditions, such as cardiovascular diseases, diabetes mellitus, and multiple sclerosis, natural products have contributed clearly to the therapeutic protocols [ 11 , 12 , 13 , 14 ].…”

Section: Cytokinins Occurrence Structure and Identificationmentioning

confidence: 99%

Cytokinins: Wide-Spread Signaling Hormones from Plants to Humans with High Medical Potential

et al. 2022

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Nature is a rich source of biologically active novel compounds. Sixty years ago, the plant hormones cytokinins were first discovered. These play a major role in cell division and cell differentiation. They affect organogenesis in plant tissue cultures and contribute to many other physiological and developmental processes in plants. Consequently, the effect of cytokinins on mammalian cells has caught the attention of researchers. Many reports on the contribution and potential of cytokinins in the therapy of different human diseases and pathophysiological conditions have been published and are reviewed here. We compare cytokinin effects and pathways in plants and mammalian systems and highlight the most important biological activities. We present the strong profile of the biological actions of cytokinins and their possible therapeutic applications.

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Inhibition of cell-intrinsic NF-κB activity and metastatic abilities of breast cancer by aloe-emodin and emodic-acid isolated from Asphodelus microcarpus

Cited by 38 publications

References 44 publications

Pantoprazole Attenuates MAPK (ERK1/2, JNK, p38)–NF-κB and Apoptosis Signaling Pathways after Renal Ischemia/Reperfusion Injury in Rats

Pantoprazole Attenuates MAPK (ERK1/2, JNK, p38)–NF-κB and Apoptosis Signaling Pathways after Renal Ischemia/Reperfusion Injury in Rats

Carpachromene Ameliorates Insulin Resistance in HepG2 Cells via Modulating IR/IRS1/PI3k/Akt/GSK3/FoxO1 Pathway

Cytokinins: Wide-Spread Signaling Hormones from Plants to Humans with High Medical Potential

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