Inhibition of cell motility and invasion by HangAmDan-B in NCI-H460 human non-small cell lung cancer cells

Abstract

Abstract.Correlation between inhibition of cell motility and anti-invasive activity by the water extract of HangAmDan-B (HAD-B), a crude extract of eight Korean medicinal animals and plants, in NCI-H460 human non-small cell lung cancer (NSCLC) cells was investigated. Within the concentrations that were not cytotoxic, HAD-B induced significant concentrationdependent inhibition of cell motility and invasiveness of NCI-H460 cells. Treatment with HAD-B resulted in dosedependent inhibition of the activities of matrix metallo proteinase (MMP)-2 and MMP-9, and this was correlated with a decrease in expression of their mRNA and proteins, and upregulation of tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 expression. Anti-invasive activity of HAD-B was also found to be associated with increased tightness of the tight junction (TJ), as demonstrated by an increase in transepithelial electrical resistance. In addition, the present results indicated that treatment with HAD-B resulted in repression of the levels of claudin family members, which are major components of TJs that play a key role in control and selectivity of para cellular transport. Although further studies are needed, findings from the present study indicate that TJs and MMPs are critical targets of HAD-B-induced anti-invasiveness in NCI-H460 NSCLC cells.

Inhibition of lung cancer growth by HangAmDan-B is mediated by macrophage activation to M1 subtype

“…Previously, antitumor effects of HAD-B were investigated in several in vitro models ( 7 – 10 ). However, to the best of our knowledge, there is no preceding report on the in vivo antitumor effect of HAD-B.…”

“…HAD-B, composed of eight herbal ingredients in a crude ground power form ( Table I ), was obtained from the East-West Cancer Center of Dunsan Korean Hospital (Daejeon, Korea). The water extract of HAD-B was prepared as previously reported ( 7 ). Briefly, crude powder of HAD-B was extracted with 10 times (v/w) the amount of distilled water, at room temperature for 24 h. The sample was centrifuged at 1,000 × g for 30 min, filtered and lyophilized.…”

“…HangAmDan-B (HAD-B), a Korean medicinal herbal formula, consisting of eight ingredient medicinal animals and plants, has been used for the treatment of cancer patients in the clinic to stimulate immune function and activation of vital energy ( 7 ). Previous studies have reported the anti-angiogenic and anti-metastatic effects of HAD-B ( 7 , 8 ).…”

“…HangAmDan-B (HAD-B), a Korean medicinal herbal formula, consisting of eight ingredient medicinal animals and plants, has been used for the treatment of cancer patients in the clinic to stimulate immune function and activation of vital energy ( 7 ). Previous studies have reported the anti-angiogenic and anti-metastatic effects of HAD-B ( 7 , 8 ). In addition, the suppression of γ-aminobutyric acid B receptor 1 (GABABR1) and heat shock protein 27 (HSP27) expression was demonstrated as a molecular mechanism underlying the anti-cancer effect of HAD-B ( 9 , 10 ).…”

Use of black vulture (Coragyps atratus) in complementary and alternative therapies for cancer in Colombia: A qualitative study

“…First, awareness of zootherapeutic practices and, in general, use of complementary and alternative medicines must be increased. Further studies are needed to evaluate the effects of black vulture’s blood in neoplastic cells in vitro , as has been done with other plant and animal based remedies [34]. In addition, clinical outcomes of Coragyps atratus use must be evaluated in cancer patients, to determine its pharmacological properties and identify possible health hazards resulting from these types of practices, especially when animal derivatives are commercialized without adequate quality control.…”