Inhibition of chlorophyll synthesis in <i>Hordeum vulgare</i> by 3-amino 2,3-dihydrobenzoic acid (gabaculin)

Hill, Corinne M.; Pearson, Sharon A.; Smith, A. J.; Rogers, Lyndon J.

doi:10.1007/bf01119876

Cited by 62 publications

(40 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…First, GAB inhibited the accumulation of both Chl and PC. The concentration requirements for inhibition were similar to those of other systems (15,25). Second, these concentration requirements were influenced by the nutrient status of the cell.…”

Section: Introductionsupporting

confidence: 48%

“…The pyrrole product formed by condensation was determined using modified Ehrlich reagent and was quantified by absorption at 552 nm (20 Excretion of ALA Induced by GAB. Experiments with higher plant systems suggest that GAB inhibits the formation of ALA (9,10,15,29). In contrast, LA is a competitive inhibitor of ALA dehydratase (17) and promotes ALA excretion.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, GAB has been shown to inhibit ALA synthesis in higher plants (9,10,15,29). The pathway from glutamate to ALA in higher plants is under intensive investigation (4), and may proceed via 4,5-dioxovaleric acid (22,24) or via an activated glutamate (29).…”

Section: Methodsmentioning

confidence: 99%

“…The mechanism by which the second inhibitor, GAB, affects cyanobacteria is virtually unknown (12). This compound interferes with pyridoxal phosphate-linked aminotransferase activity (25,26), and inhibits Chl biosynthesis in higher plants (9,10,15,29).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Effects of Gabaculine on Pigment Biosynthesis in Normal and Nutrient Deficient Cells of Anacystis nidulans

Guikema¹,

Freeman²,

Fleming³

1986

Plant Physiol.

View full text Add to dashboard Cite

Pigment biosynthesis in the cyanobacterium, Anacystis nidulans, was examined in the presence of gabaculine (5-amino-1,3-cyclohexadienylcarboxylic acid). At 20 micromolar, this inhibitor blocked the biosynthesis of both chlorophyll and phycocyanin. Analogs of gabaculine were not effective as inhibitors of chlorophyll or phycocyanin biosynthesis. Iron-and phosphate-deficient cultures were 2-to 4-fold more sensitive to the inhibitor than were normal or nitrate-deficient cultures. Inhibition resulted in the excretion of a mixture of organic acids by the cells. 5-Aminolevulinic acid was a principle component of the mixture, identified by thin layer chromatography. Excretion of 6-aminolevulinic acid occurred following a brief lag after gabaculine addition. It remained linear for nearly 24 hours and was dependent upon illumination. However, high light inhibited excretion. Apparently, gabaculine blocks chlorophyll biosynthesis after the formation of 6-aminolevulinic acid in cyanobacteria.Recovery of cyanobacteria from iron deficiency is accompanied by a reproducible pattern of photosynthetic membrane repair. When iron is restored to a chlorotic culture, there is a 3 to 5 h lag, followed by the synthesis and insertion of Chl proteins into existing membranes (23). At later times, the assembly of new membrane becomes apparent (27). The factors which regulate the assembly of Chl proteins under these conditions are not well understood. Iron starvation reduces the synthesis of protoporphyrins, presumably by feedback inhibition due to an accumulation of protoheme (5,8,28). Since Chl is a component of several thylakoid membrane polypeptides, decreased Chl availability may play a regulatory role in Chl protein assembly.The differential effects of Chl availability and of intracellular iron during recovery from iron deficiency have previously been examined using two inhibitors of Chl biosynthesis (12). One of these, LA2, is a competitive inhibitor of ALA dehydratase (17,18), and effectively blocks protoporphyrin biosynthesis in both normal and iron-deficient cyanobacteria (1 1). The mechanism by which the second inhibitor, GAB, affects cyanobacteria is virtually unknown (12). This compound interferes with pyridoxal phosphate-linked aminotransferase activity (25,26), and inhibits Chl biosynthesis in higher plants (9,10,15,29).In this report, we have examined the effects of GAB on Our findings consist of three pertinent observations. First, GAB inhibited the accumulation of both Chl and PC. The concentration requirements for inhibition were similar to those of other systems (15,25). Second, these concentration requirements were influenced by the nutrient status of the cell. Finally, GAB induced the excretion of organic acids, a major excretion product being ALA. This suggested that GAB inhibits Chl biosynthesis in cyanobacteria at a site following ALA formation. MATERIALS AND METHODSCells of Anacystis nidulans R2 were grown in shaking culture as previously described (13). Axenic cultures were monitored microscopically and by...

show abstract

Section: Introductionsupporting

confidence: 48%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Effects of Gabaculine on Pigment Biosynthesis in Normal and Nutrient Deficient Cells of Anacystis nidulans

Guikema¹,

Freeman²,

Fleming³

1986

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…The combined supernatants were diluted ten-fold with 100 % acetone, and the absorbance was measured at 645 and 663 nm (Spano et al 2003). Chlorophyll a and b concentrations were calculated using the equations of Hill et al (1985).…”

Section: Methodsmentioning

confidence: 99%

Effects of Paraquat-induced oxidative stress on antioxidants and Chlorophyll Fluorescence in stay-green wheat (Triticum aestivum L.) flag leaves

et al. 2014

View full text Add to dashboard Cite

Paraquat, a highly toxic herbicide, induces the production of reactive oxygen species in the cell. Paraquat resistance in stay-green wheat (Triticum aestivum L.) was investigated. Two cultivars, CN12 and CN17, were found to have a higher photo-oxidative resistance than that of common wheat MY11, as measured with antioxidant indexes and chlorophyll a fluorescence. The flag leaves of both CN12 and CN17 maintained a relatively high antioxidant enzyme activity, chlorophyll content, and photosynthetic index and a low level of lipid degradation after 24 h of treatment with 10 µM paraquat. The Stay-green wheat cultivars CN12 and CN17 had a high resistance to paraquat-induced photo-oxidative stress, which can potentially alleviate some of the damage induced by environment pollution.

show abstract

Chlorophylls

Willows

2018

Annual Plant Reviews Online

View full text Add to dashboard Cite

show abstract

Inhibition of chlorophyll synthesis in Hordeum vulgare by 3-amino 2,3-dihydrobenzoic acid (gabaculin)

Cited by 62 publications

References 24 publications

Effects of Gabaculine on Pigment Biosynthesis in Normal and Nutrient Deficient Cells of Anacystis nidulans

Effects of Gabaculine on Pigment Biosynthesis in Normal and Nutrient Deficient Cells of Anacystis nidulans

Effects of Paraquat-induced oxidative stress on antioxidants and Chlorophyll Fluorescence in stay-green wheat (Triticum aestivum L.) flag leaves

Chlorophylls

Contact Info

Product

Resources

About