Inhibition of constitutive NFκB activity by the anti-inflammatory sesquiterpene, parthenolide slows cell growth and increases radiation sensitivity

Differential induction and activation of NF‐κB transcription complexes in radiation‐induced chromosomally unstable cell lines

“…This also agrees with work conducted in our own laboratory, showing a lack of sensitivity to ionizing radiation and mitomycin C in chromosomally unstable clones [Limoli et al, 2001;Huang et al, 2003a]. However, Mendonca et al [2003] have preliminary evidence for increased radiosensitivity and altered cell cycle profiles when a chemical inhibitor is used, and there are also data showing that the inhibition of IkBa phosphorylation can lead to radiosensitization of human glioma cells [Ding et al, 2003]. Despite the failure of all cells to become radiosensitive upon transduction with a dominant-negative IkB inhibitor, decreases in overall clonogenicity in several cell lines have been noted [Pajonk et al, 1999].…”

Dichloroacetate alters Warburg metabolism, inhibits cell growth, and increases the X-ray sensitivity of human A549 and H1299 NSC lung cancer cells

“…Cells in exponential growth phase were harvested and plated in T-75 and T-25 flasks (Corning) for stock propagation and experiments, respectively, by our standard methods [11,32,33]. The flasks were placed in a 37°C incubator with 5% CO 2 levels and 85% humidity (Forma Steri-Cult HEPA Class 100 CO 2 incubator, Thermo Scientific).…”

“…A growth curve of cell counts versus time was generated for treated and nontreated samples at 24, 48, 72, and 96 h. The growth curves for A549 and H1299 cells with 0 and 50 mM DCA were graphically analyzed and the population doubling times determined by our standard methods [11,32,33]. To assess the effect of DCA on plating efficiency (PE), i.e., colony formation, adherent cells from the A549 and H1299 treatment flasks above were trypsinized, counted, and plated at appropriate numbers for colony formation assays by our standard methods [11,32,33].…”

“…Stained colonies with at least 50 cells per colony were scored. PE was defined as the ratio of scored colonies to number of cells plated [11,32,33].…”

“…The X-ray machine had a 0.5 mm of Cu filtration and the source to target distance was 33 cm, which resulted in a dose rate of 62.8 cGy/min. The specimens were allowed to recover for 24 h and the adherent cells were trypsinized, counted, and plated at appropriate numbers for colony formation assays by our standard methods [11,32,33]. The colonies were allowed to grow for 10 days before fixation with 70% ethanol followed by crystal violet staining (3%).…”