Inhibition of cytokine accumulation and bacterial growth during storage of platelet concentrates at 4° C with retention of in vitro functional activity

Currie, Laura; Harper, James R.; Allan, Harriet E; Connor, Jerome

doi:10.1046/j.1537-2995.1997.37197176946.x

Cited by 63 publications

(42 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have also shown that metabolic suppression can result in preservation of PLT function, 50 which may also provide an explanation for the maintenance of aggregation in cold-stored PLTs. In addition, refrigerated storage reduces bacterial growth, 51,52 which is one of the primary reasons that the shelf life of PLTs is so limited.…”

Section: Discussionmentioning

confidence: 99%

Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

et al. 2016

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

et al. 2016

View full text Add to dashboard Cite

show abstract

“…[23][24][25] Unfortunately, the coldinflicted changes in GPIbα collectively defined as GPIbα clustering prevents rapid introduction into transfusion medicine. The present results describe the molecular mechanism of cold-induced GPIbα clustering.…”

Section: Discussionmentioning

confidence: 99%

Improved platelet survival after cold storage by prevention of glycoprotein Ib clustering in lipid rafts

et al. 2012

View full text Add to dashboard Cite

The online version of this article has a Supplementary Appendix. BackgroundStoring platelets for transfusion at room temperature increases the risk of microbial infection and decreases platelet functionality, leading to out-date discard rates of up to 20%. Cold storage may be a better alternative, but this treatment leads to rapid platelet clearance after transfusion, initiated by changes in glycoprotein Ibα, the receptor for von Willebrand factor. Design and MethodsWe examined the change in glycoprotein Ibα distribution using Förster resonance energy transfer by time-gated fluorescence lifetime imaging microscopy. ResultsCold storage induced deglycosylation of glycoprotein Ibα ectodomain, exposing N-acetyl-Dglucosamine residues, which sequestered with GM1 gangliosides in lipid rafts. Raft-associated glycoprotein Ibα formed clusters upon binding of 14-3-3ζ adaptor proteins to its cytoplasmic tail, a process accompanied by mitochondrial injury and phosphatidyl serine exposure. Cold storage left glycoprotein Ibα surface expression unchanged and although glycoprotein V decreased, the fall did not affect glycoprotein Ibα clustering. Prevention of glycoprotein Ibα clustering by blockade of deglycosylation and 14-3-3ζ translocation increased the survival of cold-stored platelets to above the levels of platelets stored at room temperature without compromising hemostatic functions. ConclusionsWe conclude that glycoprotein Ibα translocates to lipid rafts upon cold-induced deglycosylation and forms clusters by associating with 14-3-3ζ. Interference with these steps provides a means to enable cold storage of platelet concentrates in the near future.Key words: platelets, cold storage, glycoprotein Ibα, 14-3-3ζ, lipid raft, clustering. Ibα clustering in lipid rafts. Haematologica 2012;97(12):1873-1881. doi:10.3324/haematol.2012 This is an open-access paper. Citation: Gitz E, Koekman CA, van den Heuvel DJ, Deckmyn H, Akkerman JW, Gerritsen HC and Urbanus RT. Improved platelet survival after cold storage by prevention of glycoprotein Improved platelet survival after cold storage by prevention of glycoprotein Ibα clustering in lipid rafts ABSTRACT© F e r r a t a S t o r t i F o u n d a t i o n

show abstract

“…Refrigerated storage of platelet products would be predicted to dramatically reduce the risk of bacterial growth [6, 7], permitting extended storage of platelets and bolstering product inventories. Competing technologies now under development might ultimately undermine these potential benefits [8].…”

mentioning

confidence: 99%

Novel and unexpected clearance mechanisms for cold platelets

Rumjantseva

Hoffmeister

2010

Transfusion and Apheresis Science

View full text Add to dashboard Cite

Storage at room temperature is limited to 5 days because of the risk of bacterial growth and loss of platelet functionality. Platelet refrigeration remains impossible, because once chilled, platelets are rapidly removed from circulation. Chilling platelets (<4 h) clusters glycoprotein (GP) Ibα receptors, and β2 integrins on hepatic macrophages recognize clustered βGlcNAc residues leading to rapid clearance of acutely chilled platelets. Prolonged refrigeration increases the exposure of galactose residues such that, unexpectedly, hepatocytes remove platelets using their asialoglycoprotein receptors. Here we review current knowledge of the mechanisms of platelet removal, the existing knowledge of refrigerated platelet function, and methods to preserve platelet concentrates long-term for transfusion.

show abstract

Inhibition of cytokine accumulation and bacterial growth during storage of platelet concentrates at 4° C with retention of in vitro functional activity

Cited by 63 publications

References 29 publications

Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

Improved platelet survival after cold storage by prevention of glycoprotein Ib clustering in lipid rafts

Novel and unexpected clearance mechanisms for cold platelets

Contact Info

Product

Resources

About