Inhibition of Cytosolic Phospholipase A2α (cPLA2α) by Medicinal Plants in Relation to Their Phenolic Content

Abstract

Abstract:The cytosolic phospholipase A 2 α (cPLA 2 α) is one of the potential targets for anti-inflammatory drugs, since this enzyme plays a key role in the inflammation processes seen in health disorders, like asthma, allergic reactions, arthritis and neuronal diseases. In this study, cPLA 2 α inhibition by 43 methanol extracts from medicinal plants rich in polyphenols was determined. The eight most active extracts were derived from Ribes nigrum (IC 50 of 27.7 µg/mL), Ononis spinosa (IC 50 of 39.4 µg/mL), Urtica dioica (IC 50 of 44.32 µg/mL), Betula sp. (IC 50 of 58.02 µg/mL), Sanguisorba officinalis (IC 50 of 76.25 µg/mL), Orthosiphon stamineus (IC 50 of 78.83 µg/mL), Petasites hybridus (IC 50 of 81.02 µg/mL) and Tussilago farfara (IC 50 of 123.28 µg/mL). Additionally, the antioxidant activities of these extracts were determined with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and their phenolic content with the Folin-Ciocalteu reagent. Antioxidant activity showed a non-linear, positive correlation to the phenolic content, but no correlation of PLA 2 α inhibition with phenolic content could be established. This study provides evidence that cPLA 2 α may be a relevant target for anti-inflammatory agents.

Phytochemical Profile and Microbiological Activity of Some Plants Belonging to the Fabaceae Family

“…The development of E. coli colonies was inhibited by RP and OS and was stimulated by CV and MO (RP ↓ > OS ↓ > CV ↑ > MO ↑). The good antibacterial effect against E. coli was also demonstrated [30,32] for RP and MO [50]. In the case of MO, a medium effect on E. coli was observed [51], while our studies are supported by Ferrente et al (2020) [15], who proved a promising effect of OS on three E. coli strains, with concentrations ranging from 31-250 µg/mL.…”

“…Higher values of phenolic compounds in the flowers (0.77 mg GAE/mL) were observed compared to syrup 60 • Brix (0.06 mg GAE/mL) and R. pseudoacacia syrup 70 • Brix (0.14 mg GAE/mL) [31]. Serbian RP showed values of 74.28 ± 1.73 for TPC (mg GAE/g) [29], while Arnold et al (2015) [32] reported a phenolic content of 20.55 ± 2.56 mg GAE/g in the OS extract.…”

A Comparative Analysis of the Anatomy, Phenolic Profile, and Antioxidant Capacity of Tussilago farfara L. Vegetative Organs

“…The lowest antioxidant capacity was observed for the subterranean parts sample collected from the Vatra Dornei extract, confirmed by all 3 methods used. In the literature, no quantification was found to be directly comparable through association of methodology and plant parts analyzed; however, the qualitative comparison of leaves (higher antioxidant capacity) and roots (lower antioxidant capacity) aligned our research with previous reports (summarized in the Supplementary Materials Section in Table S1) [12,14,[35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50].…”

Changes in the phenolic compounds profile, antioxidant and anti-melanogenic activity from organs of Petasites japonicas under different extraction methods

“…For P. japonicus leaf extracts, there was no large difference in antioxidant and anti-melanogenic activities according to the extraction method. The methanol extract of P. hybridus leaf contained higher level of TPC (122.61 mg GAE/g extract; Arnold et al, 2015) compared with P. japonicus leaf extract (Table 2). However, it has been shown that TPC in the methanol extract of P. japonicus was 218.32 mg GAE/g extract (Kim et al, 2016).…”