Inhibition of degranulation and cytokine production in bone marrow-derived mast cells by hydrolyzed rice bran

Hoshino, Yuka; Hirashima, Naohide; Nakanishi, Mamoru; Furuno, Tadahide

doi:10.1007/s00011-010-0173-9

Cited by 16 publications

(20 citation statements)

References 35 publications

(44 reference statements)

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“…The study indicated that RBPS2a has the ability to induce red blood cell lysis and complement consumption through residual complement activity. In another study, different fraction of AX was extracted from rice bran using carbohydrate hydrolysing enzymes for a longer period [128]. The extracted AX had a low molecular weight and structure similar to MGN-3.…”

Section: Immunomodulatory Effects Of Rice Bran Polysaccharidesmentioning

confidence: 99%

Health-related effects and improving extractability of cereal arabinoxylans

Fadel

Mahmoud

Ashworth

et al. 2018

International Journal of Biological Macromolecules

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Arabinoxylans (AXs) are major dietary fibers. They are composed of backbone chains of β-(1-4)-linked xylose residues to which α-l-arabinose are linked in the second and/or third carbon positions. Recently, AXs have attracted a great deal of attention because of their biological activities such as their immunomodulatory potential. Extraction of AXs has some difficulties; therefore, various methods have been used to increase the extractability of AXs with varying degrees of success, such as alkaline, enzymatic, mechanical extraction. However, some of these treatments have been reported to be either expensive, such as enzymatic treatments, or produce hazardous wastes and are non-environmentally friendly, such as alkaline treatments. On the other hand, mechanical assisted extraction, especially extrusion cooking, is an innovative pre-treatment that has been used to increase the solubility of AXs. The aim of the current review article is to point out the health-related effects and to discuss the current research on the extraction methods of AXs.

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Section: Immunomodulatory Effects Of Rice Bran Polysaccharidesmentioning

confidence: 99%

Health-related effects and improving extractability of cereal arabinoxylans

Fadel

Mahmoud

Ashworth

et al. 2018

International Journal of Biological Macromolecules

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“…15) After 4 weeks of culture, >95% of the viable cells were confirmed to be mast cells, as assessed by acidic toluidine blue staining. BALB/c mice were housed under standard conditions and in compliance with Aichi Gakuin University guidelines for the use of experimental animals, and all animal experiments were performed after approval by the Animal Care and Use Committee of Aichi Gakuin University.…”

Section: Methodsmentioning

confidence: 99%

“…Ratios were converted to Ca 2+ concentrations by a previously described procedure. 15,17) Degranulation Assay The degranulation of mast cells was monitored by measuring the activity of granule-stored enzyme β-hexosaminidase secreted into cell supernatants. for 1 h at 37°C.…”

Section: +mentioning

confidence: 99%

“…Supernatants were collected and absolute amounts of TNF-α released by cells were determined using a mouse TNF-α immunoassay system (R&D Systems, Minneapolis, MN, U.S.A.) as described previously. 15,17) Western Blotting To prepare whole cell lysates, BMMCs (1×10 6 cells) were suspended in cold lysis buffer (20 mM HEPES [pH 7.9], 0.1% NP-40, 50 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM dithiothreitol, 10 mM Na 3 VO 4 , 10 µg/mL phenylmethylsulfonyl fluoride, 10 µg/mL leupeptin, and 10% glycerol) and allowed to stand on ice for 30 min. 15,[17][18][19] The suspension was clarified by centrifugation (15000 rpm, 20 min) at 4°C.…”

Section: +mentioning

confidence: 99%

“…15,17) Western Blotting To prepare whole cell lysates, BMMCs (1×10 6 cells) were suspended in cold lysis buffer (20 mM HEPES [pH 7.9], 0.1% NP-40, 50 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM dithiothreitol, 10 mM Na 3 VO 4 , 10 µg/mL phenylmethylsulfonyl fluoride, 10 µg/mL leupeptin, and 10% glycerol) and allowed to stand on ice for 30 min. 15,[17][18][19] The suspension was clarified by centrifugation (15000 rpm, 20 min) at 4°C. Afterward, supernatants were solubilized by treatment with Laemmli buffer (0.125 M Tris-HCl [pH 6.8], 10% 2-mercaptoethanol, 4% sodium dodecyl sulfate (SDS), 10% sucrose, and 0.004% bromophenol blue) for 3 min at 100°C.…”

Section: +mentioning

confidence: 99%

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Kefiran Suppresses Antigen-Induced Mast Cell Activation

Furuno

Nakanishi

2012

Biological & Pharmaceutical Bulletin

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Kefir is a traditional fermented milk beverage produced by kefir grains in the Caucasian countries. Kefiran produced by Lactobacillus kefiranofaciens in kefir grains is an exopolysaccharide having a repeating structure with glucose and galactose residues in the chain sequence and has been suggested to exert many health-promoting effects such as immunomodulatory, hypotensive, hypocholesterolemic activities. Here we investigated the effects of kefiran on mast cell activation induced by antigen. Pretreatment with kefiran significantly inhibited antigen-induced Ca 2 mobilization, degranulation, and tumor necrosis factor-α production in bone marrow-derived mast cells (BMMCs) in a dose-dependent manner. The phosphorylation of Akt, glycogen synthase kinase 3β, and extracellular signal-regulated kinases (ERKs) after antigen stimulation was also suppressed by pretreatment of BMMCs with kefiran. These findings indicate that kefiran suppresses mast cell degranulation and cytokine production by inhibiting the Akt and ERKs pathways, suggesting an anti-inflammatory effect for kefiran.

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Monomer hapten and hapten‐specific IgG inhibit mast cell activation evoked by multivalent hapten with different mechanisms

et al. 2019

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Aggregation of IgE bound to high affinity IgE receptor (FcεRI) by multivalent antigen induces mast cell activation. Reportedly, disaggregation of aggregated FcεRI immediately terminated degranulation, and formation of co-ligated FcεRI and low affinity IgG receptor FcγRIIB blocked degranulation by inhibitory signal via SH2-containing inositol 5'-phosphatase 1 (SHIP1) phosphorylation. However, their molecular mechanisms to inhibit mast cell activation have been unclear in detail. Herein, we found that addition of excess monomeric hapten (TNP-alanine) to multivalent antigen (TNP-OVA)-activated rat basophilic leukemia cells and mouse bone marrow-derived mast cells induced immediate and transient Syk dephosphorylation, which was previously phosphorylated by TNP-OVA addition. Syk dephosphorylation correlated to rapidly decreased intracellular Ca 2+ concentrations ([Ca 2+ ] i ), terminated degranulation, and suppressed cytokine production through inhibition of Akt and ERK phosphorylation. Addition of hapten-specific IgG monoclonal antibody (anti-TNP IgG1) to activated mast cells induced translocation of SHIP1 to the plasma membrane and its phosphorylation, indicating that co-ligation of FcεRI and FcγRIIB after FcεRI aggregation can lead to SHIP1 activation. SHIP1 phosphorylation led to gradually decreased [Ca 2+ ] i , weak inhibition of degranulation, and strong inhibition of cytokine production. Our findings clearly show the inhibitory mechanism of cell function in activated mast cells by operating Fc receptor crosslinking.Keywords: degranulation r hapten r mast cell r phosphorylation r SHIP1

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Inhibition of degranulation and cytokine production in bone marrow-derived mast cells by hydrolyzed rice bran

Abstract: These findings suggest that HRB may have an anti-inflammatory effect by inhibiting mast cell degranulation and cytokine production.

Cited by 16 publications

References 35 publications

Health-related effects and improving extractability of cereal arabinoxylans

Health-related effects and improving extractability of cereal arabinoxylans

Kefiran Suppresses Antigen-Induced Mast Cell Activation

Monomer hapten and hapten‐specific IgG inhibit mast cell activation evoked by multivalent hapten with different mechanisms

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