Inhibition of denaturation of human gamma globulin by a mixture of l‐histidine, l‐cystine, and copper, and its clinical implication in rheumatoid arthritis

Gerber, Donald A.

doi:10.1002/art.1780190311

Cited by 21 publications

(1 citation statement)

References 41 publications

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“…Gold(I)-thiolate complexes and D-penicillamine have been hypothesised to reduce total biofluid copper levels via their interaction with Cu(II) ions bound to the N-terminal binding site located on albumin [15]. A mixture of histidine, cysteine and Cu(II) ions has been demonstrated to mimic disodium aurothiomalate in inhibiting the sulphydryl-disulfide interchange reaction-mediated denaturation of human gamma globulin, bovine serum albumin and diluted human serum [17].…”

Section: Introductionmentioning

confidence: 99%

Detection and investigation of the molecular nature of low‐molecular‐mass copper ions in isolated rheumatoid knee‐joint synovial fluid

et al. 1995

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Low-molecular-mass copper(lI) species have been detected and quantified in ultrafiltrates (n = 7) of rheumatoid synovial fluid (SF) by a highly-sensitive HPLC-based assay system with the ability to determine Cu(II) concentrations of < 10 -7 mol.dm -3. High field 1H NMR spectroscopy demonstrated that addition of Cu(II)¢,q.) to isolated samples of RA SF ultrafiltrates resulted in complexation by histidine > alanine > formate > threouine > lactate • tyrosine • phenylalauine, their effectiveness in this context being in the given order. CD spectra of Cu(H)-treated samples of intact SF exhibited absorption bands typical of copper(II)-albumin complexes, in addition to a band attributable to a low-molecular-mass histidinate complex (A~ 610 nm). Since both albumin and histidine are potent radical scavengers, these results indicate that any "OH radical generated from bound copper ions will be 'site-specifically' scavenged. ttence, low-molecular-mass copper complexes with the ability to promote the generation of "OH radical which can then escape trom the metal ion co-ordination sphere (and in turn, cause damage to critical biomolecules) appear to be absent from inflammatory SF.

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Section: Introductionmentioning

confidence: 99%