1995
DOI: 10.1152/ajplung.1995.268.2.l201
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Inhibition of dihydropyridine-sensitive calcium entry in hypoxic relaxation of airway smooth muscle

Abstract: Hypoxia dilates airways in vivo and reduces active tension of airway smooth muscle in vitro. To determine whether hypoxia impairs Ca2+ entry through voltage-dependent channels (VDC), we tested the ability of dihydropyridines to modulate hypoxia-induced relaxation of KCl- and carbamyl choline (carbachol)-contracted porcine bronchi. Carbachol- or KCl-contracted bronchial rings were exposed to progressive hypoxia in the presence or absence of 1 microM BAY K 8644 (an L-type-channel agonist). In separate experiment… Show more

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Cited by 9 publications
(9 citation statements)
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“…In hypoxia, the +/+ animals exhibited significant acinar airway dilatation as well as increased lung air and tissue volumes and surface areas, consistent with airway smooth muscle relaxation and compensatory alveolar growth, respectively. Hypoxia reduces airway smooth muscle tone partly via limiting calcium entry into cells (Vannier et al, 1995, Wetzel et al, 1992, Julia-Serda et al, 1993); the bronchodilation effect extends throughout the bronchial tree down to the distal intra-acinar airways as seen in fa/fa lungs where the normally smaller alveolar ducts became more dilated in hypoxia. The hypoxia-induced gains in septal volumes and surface areas were consistently lower in fa/fa compared to +/+ lungs, especially for type II epithelium volume.…”
Section: Discussionmentioning
confidence: 99%
“…In hypoxia, the +/+ animals exhibited significant acinar airway dilatation as well as increased lung air and tissue volumes and surface areas, consistent with airway smooth muscle relaxation and compensatory alveolar growth, respectively. Hypoxia reduces airway smooth muscle tone partly via limiting calcium entry into cells (Vannier et al, 1995, Wetzel et al, 1992, Julia-Serda et al, 1993); the bronchodilation effect extends throughout the bronchial tree down to the distal intra-acinar airways as seen in fa/fa lungs where the normally smaller alveolar ducts became more dilated in hypoxia. The hypoxia-induced gains in septal volumes and surface areas were consistently lower in fa/fa compared to +/+ lungs, especially for type II epithelium volume.…”
Section: Discussionmentioning
confidence: 99%
“…To chelate extracellular Ca 2ϩ , 1,2-bis(o-aminophenoxy)ethane-N,N,NЈ,NЈ-tetraacetate (BAPTA, 1 mM) was used in the bath solution instead of CaCl 2 (22). To identify the relative importance of different Ca 2ϩ channel subtypes in blastocyst function, dormant blastocysts were exposed to 2 M of -Conotoxin GVIA and Calciseptine, selective blockers of N-and L-type Ca 2ϩ channels (22), respectively, or to 4 M (ϩ͞Ϫ)-Bay K 8644, a selective activator of the L-type Ca 2ϩ channel (23), in the presence or absence of KCl stimulation. Images were acquired for 5 min after the addition of KCl on a Zeiss LSM 510 confocal scanning laser microscope.…”
Section: Methodsmentioning
confidence: 99%
“…Hypoxic relaxation is accompanied by a decrease in intracellular calcium and may or may not be mediated by K ATP channels (88,177). H 2 Smediated relaxation of airways appears to be independent of K ATP channels, prostaglandins, and NO (80).…”
Section: Mechanical Effects On Airway Smooth Musclementioning
confidence: 99%