Inhibition of DNA demethylation enhances plant tolerance to cadmium toxicity by improving iron nutrition

Fan, Shi Kai; Ye, Jiayuan; Zhang, Lin Lin; Chen, Hong Shan; Zhang, Hai Hua; Zhu, Ya; Liu, Xing Xing; Jin, Chong

doi:10.1111/pce.13670

Cited by 63 publications

(36 citation statements)

References 79 publications

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“…The mapping efficiency obtained is comparable to the informed in other WGBS studies [16,[48][49][50]. Likewise, the proportion of the three-methylation context in the transgenic tobaccos was similar to those reported in other plant models as soybean [51], barrel clover [7], maize [16], Arabidopsis [21,50], tomato [52], burberry [48] and watermelon [49], among others. In all these studies, the higher methylation levels were located in the CG context.…”

Section: Discussionsupporting

confidence: 87%

Whole-Genome DNA Methylation Analysis in Hydrogen Peroxide Overproducing Transgenic Tobacco Resistant to Biotic and Abiotic Stresses

Villagomez‐Aranda

García-Ortega

Torres‐Pacheco

et al. 2021

Plants

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Epigenetic regulation is a key component of stress responses, acclimatization and adaptation processes in plants. DNA methylation is a stable mark plausible for the inheritance of epigenetic traits, such that it is a potential scheme for plant breeding. However, the effect of modulators of stress responses, as hydrogen peroxide (H2O2), in the methylome status has not been elucidated. A transgenic tobacco model to the CchGLP gene displayed high H2O2 endogen levels correlated with biotic and abiotic stresses resistance. The present study aimed to determine the DNA methylation status changes in the transgenic model to obtain more information about the molecular mechanism involved in resistance phenotypes. The Whole-genome bisulfite sequencing analysis revealed a minimal impact of overall levels and distribution of methylation. A total of 9432 differential methylated sites were identified in distinct genome regions, most of them in CHG context, with a trend to hypomethylation. Of these, 1117 sites corresponded to genes, from which 83 were also differentially expressed in the plants. Several genes were associated with respiration, energy, and calcium signaling. The data obtained highlighted the relevance of the H2O2 in the homeostasis of the system in stress conditions, affecting at methylation level and suggesting an association of the H2O2 in the physiological adaptation to stress functional linkages may be regulated in part by DNA methylation.

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Section: Discussionsupporting

confidence: 87%

Whole-Genome DNA Methylation Analysis in Hydrogen Peroxide Overproducing Transgenic Tobacco Resistant to Biotic and Abiotic Stresses

Villagomez‐Aranda

García-Ortega

Torres‐Pacheco

et al. 2021

Plants

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“…It was reported that the DNA demethylase DML2 was downregulated under cold stress in tomato, which resulted in the hypermethylation of the promoter and silencing of genes involved in the biosynthesis of flavor volatiles 41 . Moreover, Cd-induced lower expression of three DNA demethylase genes ( ROS1 , DML2 and DML3 ) resulted in higher DNA methylation in Arabidopsis 39 . In this study, RT-qPCR analysis of DNA demethylase genes showed that RsROS1 was downregulated in Pb200 and upregulated in Pb_50MT, while RsDME presented higher expression levels in Pb200 than in Control, indicating that these genes may play a major role in decreasing the methylation level after MT treatment (Fig.…”

Section: Discussionmentioning

confidence: 95%

“…In rice in response to Cd stress, the methylation level of genes was changed, followed by altered expression levels, and 5-azacytidine promoted growth and Cd accumulation by enhancing the transcription of genes involved in HM transport 12 . Exposure to Cd elevated DNA methylation at the genome-wide level in Arabidopsis roots 39 . In the present study, the genome-wide DNA methylation level was increased under Pb stress and obviously reduced after 50 μM MT treatment (Fig.…”

Section: Discussionmentioning

confidence: 95%

Melatonin-induced DNA demethylation of metal transporters and antioxidant genes alleviates lead stress in radish plants

Tang

Wang

et al. 2021

Hortic Res

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Melatonin (MT) is a tryptophan-derived natural product that plays a vital role in plant response to abiotic stresses, including heavy metals (HMs). However, it remains elusive how exogenous MT mediates lead (Pb) accumulation and detoxification at the methylation and transcriptional levels in radish. In this study, decreased Pb accumulation and increased antioxidant enzyme activity were detected under MT treatment in radish. Single-base resolution maps of DNA methylation under Pb stress (Pb200) and Pb plus MT treatment (Pb_50MT) were first generated. The genome-wide methylation level was increased under Pb stress, while an overall loss of DNA methylation was observed under MT treatment. The differentially methylated region (DMR)-associated genes between Pb_50MT and Pb200 were uniquely enriched in ion binding terms, including cation binding, iron ion binding, and transition metal ion binding. Hyper-DMRs between Pb200 and Control exhibited a decreasing trend of methylation under Pb_50MT treatment. A few critical upregulated antioxidant genes (e.g., RsAPX2, RsPOD52 and RsGST) exhibited decreased methylation levels under MT treatment, which enabled the radish plants to scavenge lead-induced reactive oxygen species (ROS) and decrease oxidative stress. Notably, several MT-induced HM transporter genes with low methylation (e.g., RsABCF5, RsYSL7 and RsHMT) and transcription factors (e.g., RsWRKY41 and RsMYB2) were involved in reducing Pb accumulation in radish roots. These findings could facilitate comprehensive elucidation of the molecular mechanism underlying MT-mediated Pb accumulation and detoxification in radish and other root vegetable crops.

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“…Moreover, little information has been reported to show how Zn‐ and Cd‐induced DNA and protein methylation regulate Zn and Cd accumulation and tolerance in plants: Fan et al . (2020) showed that DNA demethylation enhances plant tolerance to Cd toxicity in A. thaliana , while Serre et al . (2020) pointed out differences in the Lys methylation of nonhistone proteins in two Cd‐sensitive species, A. thaliana and A. lyrata , and in three populations of A. halleri AU, I16 and PL22 with contrasting Cd accumulation and tolerance.…”

Section: Discussionmentioning

confidence: 99%

Heavy metals modulate DNA compaction and methylation at CpG sites in the metal hyperaccumulator Arabidopsis halleri

Galati

Gullì

Giannelli

et al. 2021

Environ and Mol Mutagen

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Excess heavy metals affect plant physiology by inducing stress symptoms, however several species have evolved the ability to hyperaccumulate metals in above‐ground tissues without phytotoxic effects. In this study we assume that at subcellular level, different strategies were adopted by hyperaccumulator versus the non‐accumulator plant species to face the excess of heavy metals. At this purpose the comet assay was used to investigate the nucleoid structure modifications occurring in response to Zn and Cd treatments in the I16 and PL22 populations of the hyperaccumulator Arabidopsis halleri versus the nonaccumulator species Arabidopsis thaliana. Methy‐sens comet assay and RT‐qPCR were also performed to associate metal induced variations in nucleoids with possible epigenetic modifications. The comet assay showed that Zn induced a mild but non significant reduction in the tail moment in A. thaliana and in both I16 and PL22. Cd treatment induced an increase in DNA migration in nuclei of A. thaliana, whereas no differences in DNA migration was observed for I16, and a significant increase in nucleoid condensation was found in PL22 Cd treated samples. This last population showed higher CpG DNA methylation upon Cd treatment than in control conditions, and an up‐regulation of genes involved in symmetric methylation and histone deacetylation. Our data support the hypothesis of a possible role of epigenetic modifications in the hyperaccumulation trait to cope with the high Cd shoot concentrations. In addition, the differences observed between PL22 and I16 could reinforce previous suggestions of divergent strategies for metals detoxification developing in the two metallicolous populations.

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Inhibition of DNA demethylation enhances plant tolerance to cadmium toxicity by improving iron nutrition

Cited by 63 publications

References 79 publications

Whole-Genome DNA Methylation Analysis in Hydrogen Peroxide Overproducing Transgenic Tobacco Resistant to Biotic and Abiotic Stresses

Whole-Genome DNA Methylation Analysis in Hydrogen Peroxide Overproducing Transgenic Tobacco Resistant to Biotic and Abiotic Stresses

Melatonin-induced DNA demethylation of metal transporters and antioxidant genes alleviates lead stress in radish plants

Heavy metals modulate DNA compaction and methylation at CpG sites in the metal hyperaccumulator Arabidopsis halleri

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