Inhibition of DNA restrictive endonucleases by aqueous nanoparticle suspension of methanophosphonate fullerene derivatives and its mechanisms

Abstract

Aqueous nanoparticle suspension of fullerene and its derivatives are currently attracting much attention. To determine the effects of aqueous nanoparticle suspension of a mono-methanophosphonate fullerene and bis-methanophosphonate fullerene (denoted as n-MMPF and n-BMPF, respectively) on the activities of DNA restrictive endonucleases, plasmid pEGFP-N1 was cleaved at a single but differently restrictive site by EcoR I, BamH I, and isozymes Cfr9 I and Xma I, respectively. Both n-MMPF and n-BMPF inhibited the activity of EcoR I, while n-BMPF exhibited stronger inhibition than n-MMPF. Addition of n-BMPF into reaction mixtures inhibited the activities of all the four enzymes, and IC 50 values for EcoR I, BamH I, Cfr9 I and Xma I were 4.3, >30, 11.7 and 8.3 μmol/L, respectively. When EcoR I was completely inhibited by n-BMPF, addition of excess amounts of pEGFP-N1 could not produce the product linear plasmid; however, increase of EcoR I amounts antagonized EcoR I inhibition of n-BMPF. Two scavengers of reactive oxygen species (ROS), mannitol and sodium azide at the concentrations of 2−10 mmol/L, did not reverse inhibition of n-BMPF, implying that this inhibition probably is not correlated to ROS. These results suggested that aqueous nano-fullerenes might act as inhibitors of DNA restrictive endonucleases. mono-and bis-methanophosphonate fullerene, aqueous nanoparticle suspension, DNA restrictive endonucleases, reactive oxygen species

Fullerenes

“…172 Study of the effects of aqueous nanoparticles suspension of a mono-methanophosphonate and bis-methanophosphonate fullerene on the activities of DNA restrictive endonucleases showed that aqueous nano-fullerenes might act as inhibitors of DNA restrictive endonucleases. 173 It was demonstrated that fullerene derivatives accelerate the growth of hair in mice and human skin, suggesting that the mechanism that potentiates hair growth in mice is similar to that in humans. 174 A new therapeutic platform showed that fullerene derivatives could inhibit the inflammatory cascade associated with phorbol 12-myristate 13-acetate-induced inflammation.…”

In VitroPolymerization of Microtubules with a Fullerene Derivative

“…14 Gharbi et al reported 15 that fullerenes were powerful antioxidants with no acute or subacute toxicities in mice. Others 16,17 and our group 18 showed that fullerene derivatives could inhibit polymerase chain reaction (PCR) through their interaction with Taq DNA polymerase. Regarding the ecological fate of carbon nanoparticles, we recently mapped 19 the biodistribution of fullerene C 70 in rice plants and reported the generational transfer of C 70 through the plant progeny assisted by natural organic matter (NOM).…”