Inhibition of DNA Synthesis and Mitosis by DNA Affinity Proteins Released from Thymus Cells during in Vitro Incubation

Miyata, Shogo; Kihara, Hirozi K.

doi:10.1247/csf.4.275

Cited by 3 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein released from both thymus cells (Miyata and Kihara, 1979) and spleen cells, as described here, was observed to bind ss-DNA and to inhibit the DNA synthesis of various cells. The major fractions of both the proteins from thymus and spleen cells migrated to almost identical positions on polyacrylamide gel electrophoresis (unpublished data).…”

supporting

confidence: 54%

“…It has also been reported that culture supernatants of antigen-and mitogen-stimulated lymphocytes are involved in the sensitization of lymphocytes for antibody production, cytotoxic activity, and regulation of cellular growth in lymphocytes and/or nonlymphocytes (David and David, 1972;Miller, 1975;Katz, 1977;Waksman, 1979). Culture medium of thymus cells contains proteins which bind to DNA and inhibit DNA synthesis of cultured cells and the proteins are partially purified (Miyata and Kihara, 1979). We have isolated a spleen cell-derived inhibitor of DNA synthesis similar to that released by thymus cells.…”

mentioning

confidence: 99%

“…The methods for obtaining IPDS from culture supernatant of spleen cells were carried out by modification of those reported in our previous paper (Miyata and Kihara, 1979). The cells were incubated at a density of 1-3 X l o 7 cellslml in Eagle's minimal essential medium (MEM) for F hours at 37°C.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Selective inhibition of DNA synthesis by a protein released from spleen cells

Miyata

Kihara

1982

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

Spleen cells cultured release proteins into the culture medium, some of which shows both binding activity to single stranded DNA (ss-DNA) and inhibitory activity to 3H-thymidine incorporation into cells. The inhibitory protein of DNA synthesis was purified to near homogeneity by ammonium sulfate precipitation, ss-DNA-cellulose affinity chromatography, and gel filtration chromatography. The molecular weight of the major band of this protein, as estimated by sodium dodecyl-sulfate gel electrophoresis, was approximately 14,000. The effect of the protein was compared with various cell lines and primary cell culture, and it was found that the protein strongly inhibited DNA synthesis of HeLa, L, and HEp2 cells, but only inhibited to a minor extent synthesis of Ehrlich cells and primary culture of mouse embryo.

show abstract

supporting

confidence: 54%

mentioning

confidence: 99%

See 1 more Smart Citation

Selective inhibition of DNA synthesis by a protein released from spleen cells

Miyata

Kihara

1982

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lopatin and Ranney [10] reported a low molecular inhibitor for unstimulated rat thymocytes from unstimulated rat spleen cells cultured with or without a low concentration of calf serum. From unstimulated mouse thymus cells cultured for 3 h in serum-free minimal essential medium (MEM) an inhibitor for nonactivated spleen cells was recently re ported [11].…”

Section: Discussionmentioning

confidence: 99%

Supernatant Factors from Short-Term Cultures of Thymocytes: Effect on DNA Synthesis

Nordlind¹

1981

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Supematants from cultures of unstimulated guinea pig thymocytes were investigated on allogenous thymocytes as regards conditioning effect. DNA synthesis was measured by incorporation of ³H-thymidine. Supematants taken after different incubation times up to 24 h were used. When used without concentration all supematants showed stimulating activity. After concentration and fractionation, a stimulating factor was found in supematants taken after a culture time of 24 h. This factor had a molecular weight above or equal to 70,000 daltons as indicated by gel chromatography. In the same supematants also an inhibiting factor was indicated.

show abstract

“…We have found that thymus cells release proteins which have affinity for single stranded DNA (ss-DNA) during in vitro incubation (19). Thus we labeled the ss-DNA-binding proteins with radioactive amino acids and studied the characteristics of these proteins.…”

mentioning

confidence: 99%