Inhibition of DNA synthesis by griseolutein in Escherichia coli through a possible interaction at the cell surface

Root hair-endophyte stacking (RHESt) in an ancient Afro-Indian crop creates an unusual physico-chemical barrier to trap pathogen(s)

“…Phenazines exhibit potent antifungal activity, in particular against soil pathogens including Fusarium species 71-73 . Here M6 was observed to produce at least two distinct phenazines, D-alanyl-griseolutein and griseolutein A which previously shown to have anti-microbial activities 74 . Phenazines lead to the accumulation of reactive oxygen species in target cells, due to their redox potential 75,76 .…”

Mechanism of Action of Tetrocarcin A

“…As a similar example to this, M. Hori et al reported that griseolutein, which inhibits thymidine incorporation in E. coli in vivo (that is, inhibits DNA synthesis), did not cause inhibition of DNA synthesis in plasmolized E. coli cells. 19 ) The effects on the cell membrane are supported by the fact that leakage of cellular constituents was promoted by tetrocarcin A. Chlorothricin which had an analogous aglycone to tetronolide causes alteration of the ion transport system through interaction with membrane phospholipids of B. subtilis,20) and tetrocarcin A may also have a similar action to chlorothricin.…”