Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates

Maenz, Claudia; Loscher, Christine E.; Iwanski, Alicja; Bruns, Michael

doi:10.1099/vir.0.2008/003541-0

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…The procedures were already described in more detail [ 45 ]. Briefly, the protein content was calculated using the Bio-Rad protein assay with the Bradford Reagent [ 46 ].…”

Section: Methodsmentioning

confidence: 99%

T-cell epitope strength in WAP-T mouse mammary carcinomas is an important determinant in PD1/PD-L1 immune checkpoint blockade therapy

et al. 2016

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Using the SV40 transgenic WAP-T/WAP-TNP mouse models for mammary carcinomas, we compared the response to immune checkpoint blockade therapy in tumor mice expressing either SV40 T-antigen containing the LCMV NP-epitope (T-AgNP in WAP-TNP mice), or the unmodified T-antigen (T-Ag in WAP-T mice). Specifically, we asked, whether the presence of the highly immunogenic NP-epitope in T-AgNP influences this response in comparison to the weakly immunogenic T-cell epitopes of T-Ag in WAP-T tumor mice. Treatment of WAP-TNP tumor mice with either anti-PD1 or anti-PD-L1 antibodies led to tumor regression, with anti-PD-L1 treatment being more effective. However, tumors had fully re-appeared after 21 days, indicating that CTL exhaustion had been rapidly re-established. Surprisingly, the same treatment applied to WAP-T tumor mice resulted in a significantly prolonged period of tumor regression. We provide evidence that in contrast to the weak antigenic stimuli exerted by T-cell epitopes of T-Ag, the strong antigenic stimulus of the NP-epitope in T-AgNP has a dual effect: (i) a rapid generation of active NP-specific CTLs, accompanied (ii) by accelerated CTL exhaustion. Our data support the hypothesis that the immunogenicity of tumor antigen T-cell epitopes strongly influences the success of immune checkpoint blockade therapy.

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“…The procedures were already described in more detail [ 45 ]. Briefly, the protein content was calculated using the Bio-Rad protein assay with the Bradford Reagent [ 46 ].…”

Section: Methodsmentioning

confidence: 99%

T-cell epitope strength in WAP-T mouse mammary carcinomas is an important determinant in PD1/PD-L1 immune checkpoint blockade therapy

et al. 2016

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“…Here, we could demonstrate that an inhibition of NFAT activation by purified SVPs can be excluded. For this reason, the objective of our next studies will concentrate on purified e-antigen, which is now available (47).…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Nuclear Transcription Factor of Activated T Cells by Duck Hepatitis B Virus

et al. 2009

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During infection with hepadnaviruses besides the infectious agent a high number of subviral particles without nucleocapsids are produced, which are able to change the infection dramatically. In addition, it was observed that the activation of the nuclear factor of activated T cells, regulated usually in cells of the immune system, was strongly influenced after infection. When primary duck liver cells were infected with purified virions of duck hepatitis B virus the activation of this factor was reduced in a similar way as it was achieved by inhibition of calcineurin, a cellular phosphatase necessary to control the factor, whereas the addition of subviral particles inhibited this reduction. It was found that the large surface protein of the virus was responsible for the reduced activity. Although this protein was embedded in similar amounts into the envelopes of both particles, only virions were able to inhibit the activity of the nuclear factor. An explanation of the different performances of the particles in primary duck liver cells apparently depends on the individual mode of insertion of the large surface proteins into the viral membrane. Furthermore, the nuclear factor of activated T cells could only be detected in liver sinusoidal endothelial cells, which was shown being attracted by virions but not by subviral particles.

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“…The procedures were already described in more details [ 37 ]. Briefly, the protein content was calculated using the Bio-Rad protein assay with the Bradford Reagent [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

An inducible transgenic mouse breast cancer model for the analysis of tumor antigen specific CD8+ T-cell responses

et al. 2015

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Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates

Cited by 4 publications

References 38 publications

T-cell epitope strength in WAP-T mouse mammary carcinomas is an important determinant in PD1/PD-L1 immune checkpoint blockade therapy

T-cell epitope strength in WAP-T mouse mammary carcinomas is an important determinant in PD1/PD-L1 immune checkpoint blockade therapy

Modulation of the Nuclear Transcription Factor of Activated T Cells by Duck Hepatitis B Virus

An inducible transgenic mouse breast cancer model for the analysis of tumor antigen specific CD8+ T-cell responses

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