Inhibition of Embryonic HSP 90 Function Promotes Variation of Cold Tolerance in Zebrafish

Han, Bingshe; Luo, Juntao; Jiang, Penglei; Li, Yán; Wang, Qiong; Bai, Yajing; Chen, Jing; Wang, Jian; Zhang, Junfang

doi:10.3389/fgene.2020.541944

Cited by 8 publications

(4 citation statements)

References 55 publications

(59 reference statements)

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“…(Basu et al, 2002). Strong evidence suggests that HSPs have critical roles in helping fish cope with environmental change (Weber and Bosworth, 2005), but 12 HSP genes were down-regulated differentially expressed in this study, and the differential expression levels of HSP genes seem to be different in different fish coping with cold stress, e.g., the HSP genes were up-regulated expressed in channel catfish, L. lota lota and Puntius tetrazona (Weber and Bosworth, 2005;Liu L. L. et al, 2020;Yang et al, 2021), while down-regulated expressed in O. latipes, D. rerio, and Oratosquilla oratoria (Ikeda et al, 2017;Lou et al, 2019;Han et al, 2020). In a similar situation, the focal adhesion pathway of Gasterosteus aculeatus was down-regulated expressed (Metzger and Schulte, 2018), which was similar to the result of H. nehereus, while L. polyactis was up-regulated expressed (Liu F. et al, 2020).…”

Section: Protein Modificationmentioning

confidence: 60%

“…This result was similar to the pathway enriched by other fish under cold stress, like the proteasome pathway in D. rerio (Long et al, 2013) and Pampus argenteus (Zhang et al, 2022), and protein processing in endoplasmic reticulum pathway in D. rerio (Long et al, 2013), Lota lota lota (Yang et al, 2021), and O. latipes (Ikeda et al, 2017). The ubiquitin proteasome system catalyzes the great majority of the protein degradation, including both the rapid degradation of misfolded and regulation proteins (Zhao et al, 2015), therefore, the ubiquitin protease system may clear the misfolded proteins and accelerate the renewal of damaged cells for H. nehereus under low-temperature environments (Han et al, 2020). Although the endoplasmic reticulum is the primary place of protein synthesis, it is also the site of protein degradation (Christianson and Ye, 2014).…”

Section: Protein Modificationmentioning

confidence: 99%

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Regulating Strategies of Transcription and Alternative Splicing for Cold Tolerance Harpadon nehereus Fish

et al. 2022

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In recent years, Harpadon nehereus gradually become a dominant species with great potential for exploitation in the East China Sea, and it is worth investigating whether H. nehereus would tolerate cold stress to continue to expand into the colder northern waters. The molecular regulation level is favorable evidence to explore the cold tolerance of H. nehereus, a total of 6,650, 1,936, and 2,772 differentially expressed genes (DEGs) in transcription regulation, and 4,409, 1,250, and 2,303 differential alternative splicing genes (DASGs) in alternative splicing regulation were identified in H. nehereus at 13, 15, and 17°C, respectively, importantly, 47 genes were identified as the key candidate genes for cold tolerance in H. nehereus. In transcription regulation, up-regulated DEGs were enriched in metabolic process terms and ribosome, spliceosome pathway, etc., while down-regulated DEGs were enriched in signal transduction terms, focal adhesion, proteoglycans in cancer pathway, etc., at 13, 15, and 17°C, respectively. In alternative splicing regulation, spliceosome, mRNA surveillance pathway, etc., were significantly enriched in DASGs. In a word, H. nehereus adapts to cold environments mainly through transcription and translation, transmembrane transport, protein modification, etc., while cold stress may also induce some diseases in H. nehereus.

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Section: Protein Modificationmentioning

confidence: 60%

Section: Protein Modificationmentioning

confidence: 99%

Regulating Strategies of Transcription and Alternative Splicing for Cold Tolerance Harpadon nehereus Fish

et al. 2022

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“…The heat shock protein 90 (HSP90) plays an essential role in the modulation of phenotypic plasticity in vertebrate development; however, the roles of HSP90 in the modulation of cold tolerance capacity in fish are still unclear [35]. A study by Han et al reports that the transient inhibition of the embryonic HSP90 function by a chemical inhibitor or by low conductivity stress promoted the variation in cold tolerance capacity in adult zebrafish [35]. The exposure to Cafodos induced a quick and temporary increase in HSP90 after 3 h, therefore confirming the main effects of the hydrogen peroxide contained in this illicit additive.…”

Section: Skinmentioning

confidence: 99%

Multi-Omics Approaches for Freshness Estimation and Detection of Illicit Conservation Treatments in Sea Bass (Dicentrarchus Labrax): Data Fusion Applications

Benedetto,

Robotti,

Belay

et al. 2024

IJMS

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Fish freshness consists of complex endogenous and exogenous processes; therefore, the use of a few parameters to unravel illicit practices could be insufficient. Moreover, the development of strategies for the identification of such practices based on additives known to prevent and/or delay fish spoilage is still limited. The paper deals with the identification of the effect played by a Cafodos solution on the conservation state of sea bass at both short-term (3 h) and long-term (24 h). Controls and treated samples were characterized by a multi-omic approach involving proteomics, lipidomics, metabolomics, and metagenomics. Different parts of the fish samples were studied (muscle, skin, eye, and gills) and sampled through a non-invasive procedure based on EVA strips functionalized by ionic exchange resins. Data fusion methods were then applied to build models able to discriminate between controls and treated samples and identify the possible markers of the applied treatment. The approach was effective in the identification of the effect played by Cafodos that proved to be different in the short- and long-term and complex, involving proteins, lipids, and small molecules to a different extent.

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“…Quite a few HSP genes have been identified in zebrafish, including several members of the HSP47, HSP70, and HSP90 families [160]. The heat shock response has been well studied in zebrafish, together with the role of HSPs during embryonic development [160][161][162][163][164]. Information on the inflammatory functions of zebrafish HSPs are scarcer but extracellular HSP60 has, for example, been shown to act as a leukocyte chemoattractant in hair cell or caudal fin injury models [165].…”

Section: Hspsmentioning

confidence: 99%

Molecular Actors of Inflammation and Their Signaling Pathways: Mechanistic Insights from Zebrafish

Leiba

Özbilgiç

Hernández

et al. 2023

Biology

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Inflammation is a hallmark of the physiological response to aggressions. It is orchestrated by a plethora of molecules that detect the danger, signal intracellularly, and activate immune mechanisms to fight the threat. Understanding these processes at a level that allows to modulate their fate in a pathological context strongly relies on in vivo studies, as these can capture the complexity of the whole process and integrate the intricate interplay between the cellular and molecular actors of inflammation. Over the years, zebrafish has proven to be a well-recognized model to study immune responses linked to human physiopathology. We here provide a systematic review of the molecular effectors of inflammation known in this vertebrate and recapitulate their modes of action, as inferred from sterile or infection-based inflammatory models. We present a comprehensive analysis of their sequence, expression, and tissue distribution and summarize the tools that have been developed to study their function. We further highlight how these tools helped gain insights into the mechanisms of immune cell activation, induction, or resolution of inflammation, by uncovering downstream receptors and signaling pathways. These progresses pave the way for more refined models of inflammation, mimicking human diseases and enabling drug development using zebrafish models.

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Inhibition of Embryonic HSP 90 Function Promotes Variation of Cold Tolerance in Zebrafish

Cited by 8 publications

References 55 publications

Regulating Strategies of Transcription and Alternative Splicing for Cold Tolerance Harpadon nehereus Fish

Regulating Strategies of Transcription and Alternative Splicing for Cold Tolerance Harpadon nehereus Fish

Multi-Omics Approaches for Freshness Estimation and Detection of Illicit Conservation Treatments in Sea Bass (Dicentrarchus Labrax): Data Fusion Applications

Molecular Actors of Inflammation and Their Signaling Pathways: Mechanistic Insights from Zebrafish

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