Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamer

Oroh, Stery Brenda; Mustopa, Apon Zaenal; Budiarti, Sri; Budiarto, Bugi Ratno

doi:10.1007/s11033-020-05822-8

Cited by 15 publications

(7 citation statements)

References 44 publications

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“…Aptamers which attached to flagella limit the bacteria movement, thus inhibiting bacteria from forming mature biofilms [28]. Antibiofilm activity assay of six types of aptamers against E. coli EPEC K1.1 showed different activities [33]. Investigate of six DNA aptamers bound to S. aureus cells in the biofilm showed one aptamer that could facilitate the accumulation of liposomes around S. aureus cells inside the biofilm [58].…”

Section: Discussionmentioning

confidence: 99%

“…The crystal violet (CV) method was used to analyze the ability of S. aureus, S. agalactiae, and E. coli, to form biofilm. The bacteria were grown and incubated at 37℃ for 16 h [33]. The total volume of 100 μl were inserted into 96-well plate consisting of 1 μl bacterial culture and 99 μl medium.…”

Section: Biofilm Formation Analysis and Antibiofilm Activity Assaymentioning

confidence: 99%

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Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

Kusumawati

Mustopa²,

Umami³

et al. 2022

Microbiol. Biotechnol. Lett.

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Aptamers are short, chemically synthesized, single-stranded DNA or RNA oligonucleotides that fold into unique three-dimensional structures. In this study, we aim to determine the antibiofilm activity and binding specificity of the six polyclonal DNA aptamers (S15K3, S15K4, S15K6, S15K13, S15K15, and S15K20) on Staphylococcus aureus BPA-12 and Escherichia coli EPEC 4. Aptamer S15K6 showed the highest percentage of antibiofilm activity against S. aureus BPA-12 (37.4%) as shown by the lowest OD 570 value of 0.313. Aptamer S15K20 showed the highest percentage of antibiofilm activity against E. coli EPEC 4 (15.4%) as shown by the lowest OD 570 value of 0.515. Aptamers S15K13 and S15K20 showed antibiofilm activities against both S. aureus BPA-12 and E. coli EPEC4, and thus potentially have broad reactivity. Furthermore, based on the binding capacity and Kd values from our previous study, the binding specificity assay of selected polyclonal DNA aptamers (S15K3 and S15K15) against S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, S. agalactiae, E. coli MHA-6, and Listeria monocytogenes were performed using qPCR. Aptamers S15K3 and S15K15 showed specific binding to S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, and S. agalactiae, but could not bind to E. coli MHA-6 and L. monocytogenes. Therefore, this study showed that the polyclonal DNA aptamers have antibiofilm activity and were able to bind to S. aureus BPA-12 and E. coli EPEC 4 bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Biofilm Formation Analysis and Antibiofilm Activity Assaymentioning

confidence: 99%

Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

Kusumawati

Mustopa²,

Umami³

et al. 2022

Microbiol. Biotechnol. Lett.

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“…It was found that the nucleic acid aptamer SELEX 10 Colony 5 could reduce the motion diameter of EPEC K1.1 and showed the highest biofilm inhibition effect. This aptamer can reduce the mRNA expression level of bacterial biofilm formation genes (e.g., curli gene, interaction, and motility), hinder EPEC K1.1's motility, and prevent bacterial biofilm formation (Oroh et al, 2020). Sengupta et al (2020) reported the effectiveness of aptamer-DNA template silver nanocluster (Ag-NC) for inhibiting P. aeruginosa biofilm formation, and Ag-NC as a sensor is expected to be a new way to detect planktonic cells and biofilm formation.…”

Section: Aptamersmentioning

confidence: 99%

Section: Strategies Against Bacterial Biofilm Removalmentioning

confidence: 99%

Understanding bacterial biofilms: From definition to treatment strategies

Zhao

Sun

Yi-pin

2023

Front. Cell. Infect. Microbiol.

128

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Bacterial biofilms are complex microbial communities encased in extracellular polymeric substances. Their formation is a multi-step process. Biofilms are a significant problem in treating bacterial infections and are one of the main reasons for the persistence of infections. They can exhibit increased resistance to classical antibiotics and cause disease through device-related and non-device (tissue) -associated infections, posing a severe threat to global health issues. Therefore, early detection and search for new and alternative treatments are essential for treating and suppressing biofilm-associated infections. In this paper, we systematically reviewed the formation of bacterial biofilms, associated infections, detection methods, and potential treatment strategies, aiming to provide researchers with the latest progress in the detection and treatment of bacterial biofilms.

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“…At present, the screened single-stranded DNA aptamer (SELEX 10 Colony 5) can specifically bind to pathogenic EPEC. 17 When using the CV assay, a dynamic test of biofilm formation related genes for antimicrobial verification, the aptamer showed good antimicrobial properties. It can effectively inhibit biofilm formation and reduce the motor ability of EPEC.…”

Section: T H Imentioning

confidence: 99%

Applications and Challenges of Bacteriostatic Aptamers in the Treatment of Common Pathogenic Bacteria Infections

Li,

Su,

et al. 2023

Biomacromolecules

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The continuous evolution and spread of common pathogenic bacteria is a major challenge in diagnosis and treatment with current biotechnology and modern molecular medicine. To confront this challenge, scientists urgently need to find alternatives for traditional antimicrobial agents. Various bacteriostatic aptamers obtained through SELEX screening are one of the most promising strategies. These bacteriostatic aptamers can reduce bacterial infection by blocking bacterial toxin infiltration, inhibiting biofilm formation, preventing bacterial invasion of immune cells, interfering with essential biochemical processes, and other mechanisms. In addition, aptamers may also help enhance the function of other antibacterial materials/drugs when used in combination. This paper has reviewed the bacteriostatic aptamers in the treatment of common pathogenic bacteria infections. For this aspect, first, bacteriostatic aptamers and their screening strategies are summarized. Then, the effect of molecular tailoring and modification on the performance of the bacteriostatic aptamer is analyzed, and the antibacterial mechanism and antibacterial strategy based on aptamers are introduced. Finally, the key technical challenges and their development prospects in clinical treatment are also carefully discussed.

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Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamer

Cited by 15 publications

References 44 publications

Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

Understanding bacterial biofilms: From definition to treatment strategies

Applications and Challenges of Bacteriostatic Aptamers in the Treatment of Common Pathogenic Bacteria Infections

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