Inhibition of enterotoxin from Escherichia coli and Vibrio cholerae by gangliosides from human milk

Otnaess, A B; Lægreid, Astrid; Ertresvåg, K

doi:10.1128/iai.40.2.563-569.1983

Cited by 115 publications

(25 citation statements)

References 25 publications

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“…Fat globules of cow's milk also contains a bovineassociated mucoprotein (BAMP) (112) that could act in a similar manner to block attachment of K99 + or other fimbriated ETEC in calves although this possibility has yet to be investigated. Similarly, analogue receptors for E. coli heat labile enterotoxin recently have been described in human milk which raises the possibility that similar factors for STa could be in bovine milk (54,106).…”

Section: Nonspecific Immunitymentioning

confidence: 89%

Enterotoxigenic Escherichia coli Infections in Newborn Calves: A Review

Acres

1985

Journal of Dairy Science

136

104

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Diarrhea caused by enterotoxigenic Escherichia coli is an infectious bacterial disease of calves that occurs during the first few days of life. The Escherichia coli that cause the disease possess special attributes of virulence that allow them to colonize the small intestine and produce an enterotoxin that causes hypersecretion of fluid into the intestinal lumen. These enterotoxigenic Escherichia coli are shed into the environment by infected animals in the herd and are ingested by newborn calves soon after birth. There is some natural immunity to enterotoxigenic Escherichia coli; however, it often fails to protect calves born and raised under modern husbandry conditions. Hence, methods have been developed to stimulate protective immunity by vaccination of the dam. The protective antibodies are transferred passively to calves through the colostrum.

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Section: Nonspecific Immunitymentioning

confidence: 89%

Enterotoxigenic Escherichia coli Infections in Newborn Calves: A Review

Acres

1985

Journal of Dairy Science

136

104

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“…In addition to GM1, CTB binds to other gangliosides, especially fucosylated GM1 . Gangliosides in human milk are thought to protect intestinal tissues from Vibrio cholerae and E. coli enterotoxins . Ganglioside content and distribution of human milk individually alter by lactation period.…”

Section: Gsls As Immune Modulatorsmentioning

confidence: 99%

The regulatory roles of glycosphingolipid‐enriched lipid rafts in immune systems

et al. 2018

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Lipid rafts formed by glycosphingolipids (GSLs) on cellular membranes play important roles in innate and adaptive immunity. Lactosylceramide (LacCer) forms lipid rafts on plasma and granular membranes of human neutrophils. These LacCer‐enriched lipid rafts bind directly to pathogenic components, such as pathogenic fungi‐derived β‐glucan and Mycobacteria‐derived lipoarabinomannan via carbohydrate‐carbohydrate interactions, and mediate innate immune responses to these pathogens. In contrast, a‐series and o‐series gangliosides form distinct rafts on CD4+ and CD8+ T cell subsets, respectively, contributing to the respective functions of these cells and stimulating adaptive immune responses through T cell receptors. These findings suggest that gangliosides play indispensable roles in T cell selection and activation. This Review introduces the involvement of GSL‐enriched lipid rafts in innate and adaptive immunity.

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“…Holmgren et al 8 ) demonstrated that the non-immunoglobulin fraction of human milk inhibits the binding of E. coli and Vibrio cholerae enterotoxin to the epithelial cells of the intestinal tract. Kolst0 Otnress et al 9 ) suggested that human milk gangliosides might protect infants against enterotoxininduced diarrhea. We have found that the predominant ganglioside in human colostrum is disialoganglioside (G 03 ) and that in mature milk is monosialoganglioside (G M3 ).lO) Thus, it would be interesting to know whether these gangliosides inhibit the adhesion of E. coli to human intestinal cells.…”

mentioning

confidence: 99%

Inhibitory Effects of Milk Gangliosides on the Adhesion ofEscherichia colito Human Intestinal Carcinoma Cells

Idota

Kawakami

1995

Bioscience, Biotechnology, and Biochemistry

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The effects of milk gangliosides and their derivatives on the adhesion of enterotoxigenic and enteropathogenic Escherichia coli to Caco-2 cells, a human intestinal carcinoma cell line, were investigated. Human milk gangliosides inhibited the adhesion of enterotoxigenic E. coli to Caco-2 cells in the same proportion, regardless of the lactational stage, but bovine milk gangliosides were less effective. The most effective inhibitor was monosialoganglioside 1 (GM1); the adhesion rate of enterotoxigenic E. coli in the presence of GM1 was less than 20% of the positive control. The adhesion of E. coli was also depressed to 31.4% by monosialoganglioside 3 (GM3). However, the inhibitory effect of disialoganglioside 3 (GD3) was less than that of GM3. GD3 lactone, ceramide lactoside, and N-acetylneuraminic acid did not inhibit E. coli adhesion to Caco-2 cells. GM3 also inhibited the adhesion of enteropathogenic E. coli to Caco-2 cells. Thus, these results suggest that GM3 possibly behaves as a physiological component in the intestinal tract of infants to protect them against enteric infections.

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Inhibition of enterotoxin from Escherichia coli and Vibrio cholerae by gangliosides from human milk

Cited by 115 publications

References 25 publications

Enterotoxigenic Escherichia coli Infections in Newborn Calves: A Review

Enterotoxigenic Escherichia coli Infections in Newborn Calves: A Review

The regulatory roles of glycosphingolipid‐enriched lipid rafts in immune systems

Inhibitory Effects of Milk Gangliosides on the Adhesion ofEscherichia colito Human Intestinal Carcinoma Cells

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