Inhibition of Epithelial-Mesenchymal Transition Maintains Stemness in Human Amniotic Epithelial Cells

Takano, Chika; Horie, Masafumi; Taiko, Isamu; Trinh, Quang Duy; Kanemaru, Kazunori; Komine‐Aizawa, Shihoko; Hayakawa, Satoshi; Miki, Toshio

doi:10.1007/s12015-022-10420-1

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…Vimentin + AECs have been reported to be increased in the vaginally delivered amnion compared with that of cesarean delivery ( 27 ). Spontaneous EMT occurs in AECs by autocrine production of TGF-β ( 28 ), and AECs undergo TGF-β–dependent EMT shortly after being cultured ( 29 ). TGF-β is also synthesized in intrauterine tissues from trophoblasts and decidua of placental tissue ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

Fetal macrophages assist in the repair of ruptured amnion through the induction of epithelial-mesenchymal transition

et al. 2022

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The premature rupture of the amniotic sac, a condition referred to as a preterm prelabor rupture of membranes (pPROM), is a leading cause of preterm birth. In some cases, these ruptured membranes heal spontaneously. Here, we investigated repair mechanisms of the amnion, a layer of epithelial cells in the amniotic sac closest to the embryo. Macrophages migrated to and resided at rupture sites in both human and mouse amnion. A process called epithelial-mesenchymal transition (EMT), in which epithelial cells acquire a mesenchymal phenotype and which is implicated in tissue repair, was observed at rupture sites. In dams bearing macrophage-depleted fetuses, the repair of amnion ruptures was compromised, and EMT was rarely detected at rupture sites. The migration of cultured amnion epithelial cells in wound healing assays was mediated by EMT through transforming growth factor–β (TGF-β)–Smad signaling. These findings suggest that fetal macrophages are crucial in amnion repair because of their ability to induce EMT in amnion epithelial cells.

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Section: Discussionmentioning

confidence: 99%

Fetal macrophages assist in the repair of ruptured amnion through the induction of epithelial-mesenchymal transition

et al. 2022

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“…In pregnancy, TGF-β1 has been found abundant at the maternal–fetal interface. Immune cells, such as Treg cells, decidual macrophages, Hofbauer cells, and particularly first-trimester trophoblast cells, also secrete TGF-β1 [ 17 , 205 , 206 , 207 , 208 , 209 , 210 ]. During pregnancy, the maternal immune system is required to tolerate the presence of the fetus, which has a distinct set of antigens that can potentially trigger an immune response.…”

Section: Tgf-β1 and Its Essential Roles In Human Body Development And...mentioning

confidence: 99%

Roles of TGF-β1 in Viral Infection during Pregnancy: Research Update and Perspectives

Trinh

Pham

Takada

et al. 2023

IJMS

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Transforming growth factor-beta 1 (TGF-β1) is a pleiotropic growth factor playing various roles in the human body including cell growth and development. More functions of TGF-β1 have been discovered, especially its roles in viral infection. TGF-β1 is abundant at the maternal–fetal interface during pregnancy and plays an important function in immune tolerance, an essential key factor for pregnancy success. It plays some critical roles in viral infection in pregnancy, such as its effects on the infection and replication of human cytomegalovirus in syncytiotrophoblasts. Interestingly, its role in the enhancement of Zika virus (ZIKV) infection and replication in first-trimester trophoblasts has recently been reported. The above up-to-date findings have opened one of the promising approaches to studying the mechanisms of viral infection during pregnancy with links to corresponding congenital syndromes. In this article, we review our current and recent advances in understanding the roles of TGF-β1 in viral infection. Our discussion focuses on viral infection during pregnancy, especially in the first trimester. We highlight the mutual roles of viral infection and TGF-β1 in specific contexts and possible functions of the Smad pathway in viral infection, with a special note on ZIKV infection. In addition, we discuss promising approaches to performing further studies on this topic.

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Reinvesting the cellular properties of human amniotic epithelial cells and their therapeutic innovations

Yang,

Lu,

Zhao

et al. 2024

Front. Immunol.

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Human amniotic epithelial cells (hAECs) have shown promising therapeutic effects in numerous studies on various diseases due to their properties such as low immunogenicity, immunomodulation, paracrine effect, and no teratoma formation in vivo. Nevertheless, there are still many problems in archiving the large-scale clinical application of hAECs, ranging from the vague definition of cell properties to the lack of clarification of the motion of actions in cell therapies, additionally, to the gap between cell quantities with limited proliferation capacity. This review provides a detailed overview of hAECs in the aspects of the lineage development of amniotic epithelial cell, cell characteristics and functional roles, ex vivo cell cultivation and expansion systems, as well as their current status and limitations in clinical applications. This review also discusses the advantages, limitations and feasibility of hAECs, and anticipates their prospects as cell therapy products, with the aim of further promoting their clinical applications.

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Inhibition of Epithelial-Mesenchymal Transition Maintains Stemness in Human Amniotic Epithelial Cells

Cited by 5 publications

References 35 publications

Fetal macrophages assist in the repair of ruptured amnion through the induction of epithelial-mesenchymal transition

Fetal macrophages assist in the repair of ruptured amnion through the induction of epithelial-mesenchymal transition

Roles of TGF-β1 in Viral Infection during Pregnancy: Research Update and Perspectives

Reinvesting the cellular properties of human amniotic epithelial cells and their therapeutic innovations

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