Inhibition of Erwinia amylovora by Bacillus nakamurai

Leathers, Timothy D.; Saunders, Lauren P.; Bowman, Michael J.; Price, Neil P. J.; Bischoff, Kenneth M.; Rich, Joseph O.; Skory, Christopher D.; Nunnally, Melinda S.

doi:10.1007/s00284-019-01845-y

Cited by 8 publications

(3 citation statements)

References 39 publications

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“…Studies show the potential of genus Bacillus, another rhizospheric bacteria well-established on the biologics market. Leathers et al (2020), using the minimum inhibitory concentration method, observed that Bacillus nakamurai at a concentration of 5%-20% (vol/vol) was effective in controlling Erwinia amylovora, a phytopathogen that affects apple and pear trees. Zhang et al (2020) found that volatile organic compounds spp.…”

Section: Resultsmentioning

confidence: 99%

Strains of Bradyrhizobium spp. in the biological control of phytopathogens in the soybean

Russiano,

Mazaro,

Russiano

et al. 2024

RCA

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Bacteria of genus Bradyrhisobium form a symbiotic relationship with legumes, promoting biological nitrogen fi xation (BNF). However, their eff ect on the biological control of disease has not been investigated. The aim of this study was to evaluate the eff ect of four strains of Bradyrhisobium spp., namely SEMIA 5080, SEMIA 5079, SEMIA 5019 and SEMIA 587, on the in-vitro control of four soil phytopathogens: Fusarium crassistipitatum, Macrophomina phaseolina, Rhisoctonia solani and Sclerotinia sclerotiorum, which cause root rot in the soybean. All the strains of Bradyrhisobium spp. and of the phytopathogens were obtained from Embrapa Soja in Londrina, Paraná. In-vitro tests were conducted using the circle method (adapted), and comparing soybean seeds inoculated with the bacteria and the phytopathogens. The dishes subjected to the circle test were evaluated using a scanning electron microscope (SEM).Two tests of antibiosis were conducted using fi ltrates of metabolites from Bradyrhisobium spp.: assessment of the minimum inhibitory concentration (MIC) and of the antibiosis in Petri ® dishes. The results were submitted to the Lilliefors test of normality, followed by ANOVA and regression analysis using the Genes software. The comparison showed that the SEMIA 5080 and SEMIA 5019 strains achieved the best control of the four phytopathogens, with a good performance by the SEMIA 5079 strain; however, the SEMIA 587 strain showed no control over the pathogens. There was morphological damage to the hyphae of each of the phytopathogens subjected to the circle method. There was no antibiosis from the fi ltered or volatile metabolites.

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Section: Resultsmentioning

confidence: 99%

Strains of Bradyrhizobium spp. in the biological control of phytopathogens in the soybean

Russiano,

Mazaro,

Russiano

et al. 2024

RCA

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“…N3 (personal communication). Other antivirulence mechanisms apart from QQ cannot be discarded in the strain N3 T , since the Bacillus species are well-known to produce numerous inhibitor compounds and some of them do not affect the viability of the pathogen (Leathers et al, 2020). An analysis in silico using the antiSMASH tool (Blin et al, 2021) indicated that the strain N3 T produced lipopeptides of the family NRPS and lassopeptides that also implicated its antivirulence mechanism.…”

Section: Growth Promotion In Tomato Plants and Biocontrol Of Phytopat...mentioning

confidence: 99%

Peribacillus castrilensis sp. nov.: A Plant-Growth-Promoting and Biocontrol Species Isolated From a River Otter in Castril, Granada, Southern Spain

et al. 2022

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A strictly aerobic, chemoheterotrophic, endospore-forming, Gram-positive, rod-shaped bacterial strain N3T was isolated from the feces of a river otter in Castril (Granada, southern Spain). It is halotolerant, motile, and catalase-, oxidase-, ACC deaminase-, and C4- and C8-lipase-positive. It promotes tomato plant growth and can reduce virulence in Erwinia amylovora CECT 222T and Dickeya solani LMG 25993T through interference in their quorum-sensing systems, although other antagonistic mechanisms could also occur. A phylogenetic analysis of the 16S rRNA gene sequence as well as the phenotypic and phylogenomic analyses indicated that the strain N3T is a novel species of the genus Peribacillus, with the highest 16S rRNA sequence similar to that of Bacillus frigoritolerans DSM 8801T (99.93%) and Peribacillus simplex DSM 1321T (99.80%). Genomic digital DNA–DNA hybridization (dDDH) between the strain N3T and Bacillus frigoritolerans DSM 8801T and Peribacillus simplex was 12.8 and 69.1%, respectively, and the average nucleotide identity (ANIb) of strain N3T and Bacillus frigoritolerans DSM 8801T and Peribacillus simplex was 67.84 and 93.21%, respectively. The genomic G + C content was 40.3 mol%. Its main cellular fatty acids were anteiso-C15:0 and iso-C15:0. Using 16S rRNA phylogenetic and in silico phylogenomic analyses, together with the chemotaxonomic and phenotypic data, we demonstrated that the type strain N3T (=CECT 30509T = LMG 32505T) is a novel species of the genus Peribacillus and the name Peribacillus castrilensis sp. nov. is proposed.

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“…Currently, the information is dispersed, which limits the advances in the field. Although B. amyloliquefaciens strains have been evaluated on a few occasions for the control of bacterial pathogens, including Erwinia spp., − Acidovorax avenae , Bacillus pumilus , and root-knot nematodes, the antifungal applications are by far the most common. − …”

Section: Introductionmentioning

confidence: 99%

Biocontrol Ability of the Bacillus amyloliquefaciens Group, B. amyloliquefaciens, B. velezensis, B. nakamurai, and B. siamensis, for the Management of Fungal Postharvest Diseases: A Review

Wang

Herrera‐Balandrano

Wang

et al. 2022

J. Agric. Food Chem.

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The Bacillus amyloliquefaciens group, composed of B. amyloliquefaciens, B. velezensis, B. nakamurai, and B. siamensis, has recently emerged as an interesting source of biocontrol agents for the management of pathogenic fungi. In this review, all the reports regarding the ability of these species to control postharvest fungal diseases have been covered for the first time. B. amyloliquefaciens species showed various antifungal mechanisms, including production of antifungal lipopeptides and volatile organic compounds, competition for nutrients, and induction of disease resistance. Most reports discussed their use for the control of fruit diseases. Several strains were studied in combination with additives, improving their inhibitory efficacies. In addition, a few strains have been commercialized. Overall, studies showed that B. amyloliquefaciens species are a suitable environmentally friendly alternative for the control of postharvest diseases. However, there are still crucial knowledge gaps to improve their efficacy and host range.

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Inhibition of Erwinia amylovora by Bacillus nakamurai

Cited by 8 publications

References 39 publications

Strains of Bradyrhizobium spp. in the biological control of phytopathogens in the soybean

Strains of Bradyrhizobium spp. in the biological control of phytopathogens in the soybean

Peribacillus castrilensis sp. nov.: A Plant-Growth-Promoting and Biocontrol Species Isolated From a River Otter in Castril, Granada, Southern Spain

Biocontrol Ability of the Bacillus amyloliquefaciens Group, B. amyloliquefaciens, B. velezensis, B. nakamurai, and B. siamensis, for the Management of Fungal Postharvest Diseases: A Review

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