Inhibition of Escherichia coli from mastitic milk by copaiba oil

Abstract

O objetivo deste estudo foi avaliar o efeito antimicrobiano de dois diferentes óleos de copaíba sobre isolados de Escherichia coli obtidos de leite mastítico. O ensaio antimicrobiano foi realizado utilizando o teste de difusão em agar, testando três soluções: um controle negativo e duas soluções dos diferentes óleos de copaíba. O teste foi realizado com 27 isolados de E. coli. Oito isolados (29.63%) foram inibidos pela solução com óleo de C. langsdorffii e sete pela solução com óleo de C. officinalis. Os resultados do presente estudo sugerem o óleo de copaiba como uma potencial fonte de novos e seletivos agentes para o tratamento da mastite, após posteriores testes em modelos in vivo. Palavras-chave: Escherichia coli, mastite, óleo de copaíba, antimicrobiano DOI: 10.5433/1679 AbstractThe aim of this study was to evaluate the antimicrobial effect of two different copaiba oils against Escherichia coli obtained from mastitic milk. An antimicrobial assay was performed using an agar diffusion test, testing three solutions: a negative control and two solutions of different copaiba oils. This test was performed with 27 mastitic E. coli isolates. Eight isolates (29.63%) were inhibited by C. langsdorffii oil solution and seven by C. officinalis oil. The results of the present in vitro study suggest that copaiba oils may be potential sources of new and selective agents for the treatment of mastitis, pending further testing in in vivo models.

Antimicrobial activity of allylic thiocyanates derived from the Morita-Baylis-Hillman reaction

“…Interesting, P. aeruginosa was the sole strain that was not sensitive to any of the thiocyanates 2 tested. This observation is suggestive of a selective action against Gram-positive rather than Gram-negative bacteria ( Pieri et al , 2011 ).…”

Effects of Copaifera duckei Dwyer oleoresin on the cell wall and cell division of Bacillus cereus

“…Pacheco et al (2006) also verified the activity of different species of Copaifera against Staphylococcus aureus and B. subtilis, but not against Gramnegative (Escherichia coli and P. aeruginosa) bacteria. However, studies with C. langsdorffii and C. officinalis oleoresins have shown inhibitory action against Escherichia coli strains recovered from mastitic milk (Pieri et al, 2011), P. aeruginosa, and Shigella flexneri (Pieri et al, 2012). Mendonça & Onofre (2009) also observed action of C. multijuga oleoresin against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and P. aeruginosa) bacteria.…”

“…Activity of oleoresins from different species (C. martii, C. reticulata and C. officinalis) has been observed against another Bacillus species, B. subtilis, with a MIC ranging from 15.62 to 31.25 mg ml 21 (Pieri et al, 2011).…”

“…Plants have been the main target of these studies, as they represent a great source of substances, many of which may have pharmacological potential. In vitro studies have described the activity of plants and their products for the treatment of microbial diseases, among them species of the genus Copaifera, which have shown antibacterial and antifungal activities in their oil (Santos et al, 2008b;Pieri et al, 2011).…”

Avaliação dos efeitos citoprotetores de compostos isolados de Copaifera langsdorffii Desf. contra citotoxidade induzida pela exposição ao metilmercúrio e ao chumbo

“…Listeria monocytogenes (Oliveira et al 2007;Santos et al 2008a;Pieri et al 2010Pieri et al , 2011Pieri et al , 2012. Também tem sido provado seu efeito larvicida contra Aedes aegypti, vetor do dengue (de Mendonça et al 2005), promastigoticida de Leishmania amazonensis (Santos et al 2008b), e inseticida contra Diabrotica speciosa, praga de plantas produtoras de frutos e grãos (Barbosa et al 2013).…”