Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity

Roe, Andrew J.; O’Byrne, Conor; McLaggan, Debbie; Booth, Ian R.

doi:10.1099/00221287-148-7-2215

Cited by 309 publications

(264 citation statements)

References 37 publications

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“…These SCFAs inhibit Escherichia coli (E. coli) and Salmonella spp. growth in vitro (31) and may impair growth by their effects on pathways, resulting in methionine depletion and homocysteine accumulation (32). The cecal pH and SCFA profiles of mice that consumed this BFMP had a clear growth-inhibitory effect on the colitogenic Enterobacteriaceae of T-bet −/− Rag2 −/− mice in vivo and in vitro.…”

Section: Discussionmentioning

confidence: 99%

Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes

Veiga

Gallini

Beal

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

201

164

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Intestinal health requires the coexistence of eukaryotic self with the gut microbiota and dysregulated host-microbial interactions can result in intestinal inflammation. Here, we show that colitis improved in T-bet −/− Rag2 −/− mice that consumed a fermented milk product containing Bifidobacterium animalis subsp. lactis DN-173 010 strain. A decrease in cecal pH and alterations in short chain fatty acid profiles occurred with consumption, and there were concomitant increases in the abundance of select lactate-consuming and butyrate-producing bacteria. These metabolic shifts created a nonpermissive environment for the Enterobacteriaceae recently identified as colitogenic in a T-bet −/− Rag2 −/− ulcerative colitis mouse model. In addition, 16S rRNA-based analysis of the T-bet −/− Rag2 −/− fecal microbiota suggest that the structure of the endogenous gut microbiota played a key role in shaping the host response to the bacterial strains studied herein. We have identified features of the gut microbiota, at the membership and functional level, associated with response to this B. lactis -containing fermented milk product, and therefore this model provides a framework for evaluating and optimizing probiotic-based functional foods.

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Section: Discussionmentioning

confidence: 99%

Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes

Veiga

Gallini

Beal

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

201

164

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“…The reduction in the cytoplasmic pH of the bacterial cell which in turn affects the purine bases (Choi et al, 2000) resulting in denaturing essential enzymes inside the cell (Roe et al, 2002), ultimately leading to the death of bacteria. Gantois et al (2006) reported that when Salmonella spp.…”

Section: Direct Bactericidal Effect Of Sodium Butyratementioning

confidence: 99%

Sodium butyrate in chicken nutrition: the dynamics of performance, gut microbiota, gut morphology, and immunity

Ahsan

Cengiz

Raza

et al. 2016

World's Poultry Science Journal

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The effect of sodium butyrate on various bodily parameters of broilers such as performance, gut microflora, gut morphology, and immunity is reviewed in order to highlight its importance as an alternative to antibiotic growth promoters. Sodium butyrate is used as a source of butyric acid, which is known for its beneficial effects in the gut in monogastrics. Sodium butyrate is available in uncoated and entericcoated forms protected with fat or fatty acid salts. Varying results in productive performance, gut microbes, and gut morphology have been reported in the literature in response to supplementation of broiler diets with uncoated and fatcoated types of sodium butyrate. However, sodium butyrate has shown pronounced effects on immunity of chickens that are not fully understood yet. Although there are contrasting results of sodium butyrate in chicken, further research is needed using the sodium butyrate coated with the salts of fatty acids.

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“…Finally, genes in a putative operon orthologous to microbial methionine transporters (CAC0984-CAC0986) were differentially upregulated after acetate stress. Studies in E. coli have shown that acetate inhibition at 8-33 mM concentration can be relieved by the addition of methionine (Han et al, 1993;Roe et al, 2002). A result of acetate stress is accumulation of the toxic intermediate homocysteine (Roe et al, 2002).…”

Section: Differential Expression Of Amino Acid Biosynthesis and Transmentioning

confidence: 99%

“…Studies in E. coli have shown that acetate inhibition at 8-33 mM concentration can be relieved by the addition of methionine (Han et al, 1993;Roe et al, 2002). A result of acetate stress is accumulation of the toxic intermediate homocysteine (Roe et al, 2002). In E. coli, homocysteine can perturb synthesis of branched-chain amino acids (Tuite et al, 2005).…”

Section: Differential Expression Of Amino Acid Biosynthesis and Transmentioning

confidence: 99%

“…Mechanisms of solvent and carboxylic acid toxicity/stress have been studied mostly in Gram-negative (Gram À ) organisms and notably in Escherichia coli (Arnold et al, 2001;Chang and Cronan, 1999;Hayashi et al, 2003;Isken and de Bont, 1998;Kirkpatrick et al, 2001;Roe et al, 2002;Russell and Diez-Gonzalez, 1998;Sardessai and Bhosle, 2002;Zaldivar and Ingram, 1999). For example, Gram À organisms have evolved adaptive mechanisms to tolerate solvents, including alterations in cell membrane, active secretion, and activation of general stress response (Isken and de Bont, 1998;Sardessai and Bhosle, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Metabolite stress and tolerance in the production of biofuels and chemicals: Gene‐expression‐based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum

Alsaker

Paredes

Papoutsakis

2010

Biotech & Bioengineering

202

189

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Metabolite accumulation has pleiotropic, toxic, or beneficial effects on cell physiology, but such effects are not well understood at the molecular level. Cells respond and adapt to metabolite stress by mechanisms largely unexplored, especially in the context of multiple and simultaneous stresses. Solventogenic and related clostridia have an inherent advantage for production of biofuels and chemicals directly from cellulosic material and other complex carbohydrates, but issues of product/metabolite tolerance and related culture productivities remain. Using DNA microarray-based gene expression analysis, the transcriptional-stress responses of Clostridium acetobutylicum to fermentation acids acetate and butyrate and the solvent product butanol were analyzed and compared in the context of cell physiology. Ontological analysis demonstrated that stress by all three metabolites resulted in upregulation of genes related to post-translational modifications and chaperone activity, and downregulation of the translationmachinery genes. Motility genes were downregulated by acetate-stress only. The general metabolite stress included upregulation of numerous stress genes (dnaK, groES, groEL, hsp90, hsp18, clpC, and htrA), the solventogenic operon aad-ctfA-ctfB, and other solventogenic genes. Acetate stress downregulated expression of the butyryl-CoA-and butyrate-formation genes, while butyrate stress downregulated expression of acetate-formation genes. Pyrimidine-biosynthesis genes were downregulated by most stresses, but purine-biosynthesis genes were upregulated by acetate and butyrate, possibly for thiamine and histidine biosynthesis. Methionine-biosynthesis genes were upregulated by acetate stress, indicating a possibly conserved stress response mechanism also observed in Escherichia coli. Nitrogen-fixation gene expression was upregulated by acetate stress. Butyrate stress upregulated many iron-metabolism genes, riboflavin-biosynthesis genes, and several genes related to cellular repair from oxidative stress, such as perR and superoxide dismutases. Butanol stress upregulated the glycerol metabolism genes glpA and glpF. Surprisingly, metabolite stress had no apparent effect on the expression of the sporulation-cascade genes. It is argued that the list of upregulated genes in response to the three metabolite stresses includes several genes whose overexpression would likely impart tolerance, thus making the information generated in this study, a valuable source for the development of tolerant recombinant strains.

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Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity

Cited by 309 publications

References 37 publications

Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes

Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes

Sodium butyrate in chicken nutrition: the dynamics of performance, gut microbiota, gut morphology, and immunity

Metabolite stress and tolerance in the production of biofuels and chemicals: Gene‐expression‐based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum

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