1984
DOI: 10.1002/j.1460-2075.1984.tb02010.x
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Inhibition of eukaryotic tRNA transcription by potential Z-DNA sequences.

Abstract: The effect of d(CA/TG)n DNA segments on tRNA transcription has been examined. Alternating purine‐pyrimidine tracts were cloned at a long distance from, adjacent to, or within the coding sequence of a tRNAPro gene from Caenorhabditis elegans and shown to be able to assume the A‐DNA conformation in vitro in physiological salt concentrations. The transcriptional level of these constructs was compared to that of normal tDNAPro by micro‐injection into Xenopus laevis oocytes. Our results show a strong inhibitory eff… Show more

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Cited by 44 publications
(21 citation statements)
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“…Other putative cis elements within region 1 + 2 for which there are precedents for involvement in negative regulation in other systems include the C/EBP, AP1 and GRE sites (Hay et al, 1989;Lucibello et al, 1990;Lopez-Cabrera et al, 1990). An alternating purine-thymidine-rich sequence and/or short repeats located 5' to the late polyadenylation signal, at nt 7407 (Farr et al, 1991), may also play a role in negative regulation (Santoro et al, 1984;Yu & Manley, 1986). We are currently defining the specific sequence(s) necessary and sufficient for silencer activity.…”
Section: Discussionmentioning
confidence: 99%
“…Other putative cis elements within region 1 + 2 for which there are precedents for involvement in negative regulation in other systems include the C/EBP, AP1 and GRE sites (Hay et al, 1989;Lucibello et al, 1990;Lopez-Cabrera et al, 1990). An alternating purine-thymidine-rich sequence and/or short repeats located 5' to the late polyadenylation signal, at nt 7407 (Farr et al, 1991), may also play a role in negative regulation (Santoro et al, 1984;Yu & Manley, 1986). We are currently defining the specific sequence(s) necessary and sufficient for silencer activity.…”
Section: Discussionmentioning
confidence: 99%
“…One argument for a functional role is the evidence that poly(GT) tracts are nonrandomly distributed in the Drosophila genome, poly(GT) sites being associated with regions of transcriptional activity (24). Other workers have found evidence that these tracts stimulate (3) or repress (27,38) transcription and stimulate recombination (41,44,48). Although these results suggest that poly(GT) tracts may be functionally important, they do not exclude the possibility that these tracts simply represent s nme byproduct of DNA metabolism in eukaryotic cells.…”
mentioning
confidence: 99%
“…In addition, the mammalian GT element has been shown to have weak transcriptional enhancer activity with the simian virus 40 (SV40) early promoter (16). The GT element is capable of forming left-handed, or Z-form, DNA (13, 17, 36), which has been suggested to play a role in the regulation of transcriptional enhancers (27), and potentially to be a negative regulatory sequence (28,30). In addition, non-B-DNA and Si-sensitive structures have been suggested to be involved in transcriptional control (see reference 39).…”
mentioning
confidence: 99%