Inhibition of fengycins on the production of fumonisin B₁fromFusarium verticillioides

Abstract

Aim: To understand the role of fengycins in regulating the fumonisin B1 (FB1) production of Fusarium verticillioides. Methods and Results: The mass ratio of FB1 to mycelia was determined in order to identify the effect of fengycins on FB1 production. It was shown that the amount of FB1 produced by unit mass mycelia decreased to 28% of the control. Results from mycelia resuspension with fengycins also demonstrated that fengycins had a potent impact on FB1 production. Gene expression patterns using quantitative reverse‐transcription PCR (RT‐PCR) revealed that the transcriptional levels of both FUM1 and FUM8 (coding enzymes for the generation of FB1) were down‐regulated with fengycin treatment. Conclusions: Fengycins could down‐regulate the transcription of some key genes involved in the production of FB1, and impair FB1 synthesis by F. verticillioides. Significance and Impact of the Study: These results further improved our understanding of fengycins as the potential candidates to control FB1 contamination in crops and food.

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

“…MTA144 (Meca et al, 2013), could encode aminotransferase FBI, showing strong fumonisin degrading activity . Hu et al reported that fengycin could inhibit the transcription of some key genes involved in the production of FB1 (Hu et al, 2009). Combined with the result in this study, we can conclude that the increase of fengycin expression could be the main cause of its enhancement of FB1-degrading ability.…”

Lipopeptides as main ingredients for inhibition of fungal phytopathogens by Bacillus subtilis/amyloliquefaciens

“…Moreover, we further exemplify some specificity regarding the target species. As occasionally reported, iturins and fengycins may interfere with some intracellular processes in fungal plant pathogens notably leading to inhibition of toxin formation (Hu et al ., ). However, the most evidenced effect of these LPs is the disruption of membrane integrity, leading to the lysis of various fungal life stages like mycelium, conidia or zoospores for oomycete pathogens (Raaijmakers et al ., ).…”

Inhibition of the Aspergillus flavus Growth and Aflatoxin B1 Contamination on Pistachio Nut by Fengycin and Surfactin-Producing Bacillus subtilis UTBSP1

“…Similarly, the antifungal activities of B. subtilis strains and other biological agents against A. flavus and AFB1-production have been reported in several studies ( Dorner et al, 1998 ; Dorner, 2004 ; Mohammadipour et al, 2009 ; Reddy et al, 2009 ; Zhang et al, 2007 ). However, contrary to our results, several studies indicated that although Bacillus spp., exhibit considerable antifungal activities against some phytopathogenic fungi but their inhibitory activities against A. flavus are no significant ( Hu et al, 2009 ; Sun et al, 2006 ).…”

“…This study demonstrates that antifungal activity of B. subtilis UTBSP1, producer of both fengycin and surfactin, is attributed to both antibiotics. However, some Bacillus strains which produce surfactin and/or fengycin couldn’t inhibit the growth of A. flavus ( Hu et al, 2009 ; Sun et al, 2006 ), whereas ( Mohammadipour et al, 2009 ) reported that B. subtilis BS119m, as a surfactin-producer in a high level, completely inhibit the growth of A. flavus . Furthermore, this study indicates that surfactin and fengycin act in a synergistic manner and provide stronger antifungal activity against A. flavus R5, similar to the synergistic antifungal manners of surfactin and iturin ( Maget-Dana and Ptak, 1990 ), surfactin and fengycin ( Ongena et al, 2007 ; Sun et al, 2006 ) and iturin and fengycin ( Romero et al, 2007 ).…”