Inhibition of gastric H⁺,K⁺‐ATPase and Helicobacter pylori growth by phenolic antioxidants of Zingiber officinale

Abstract

Ulcer is a common global problem characterized by acute gastric irritability, bleeding, etc. due to either increased gastric cell proton potassium ATPase activity (PPA) or perturbation of mucosal defence. Helicobacter pylori has been identified as a major ulcerogen in addition to oxidative stress and nonsteroidal anti-inflammatory drugs. In this paper, we report ginger-free phenolic (GRFP) and ginger hydrolysed phenolic (GRHP) fractions of ginger (Zingiber officinale) as potent inhibitors of PPA and H. pylori growth. GRFP and GRHP inhibited PPA at an IC(50) of 2.9 +/- 0.18 and 1.5 +/- 0.12 microg/mL, exhibiting six- to eight-fold better potency over lansoprazole. GRFP is constituted by syringic (38%), gallic (18%) and cinnamic (14%) acids and GRHP by cinnamic (48%), p-coumaric (34%) and caffeic (6%) acids as major phenolic acids. GRFP and GRHP further exhibited free radical scavenging (IC(50) 1.7 +/- 0.07 and 2.5 +/- 0.16), inhibition of lipid peroxidation (IC(50) 3.6 +/- 0.21 and 5.2 +/- 0.46), DNA protection (80% at 4 microg) and reducing power abilities (80-338 U/g) indicating strong antioxidative properties. GRFP and GRHP may thus be potential in-expensive multistep blockers against ulcer.

Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature

“…In fact, the cytoprotective and gastric anti-ulcer effect of ginger have been showed experimentally in early studies in rats (Yamahara et al, 1988;Wu et al, 1990). Recently, data provides evidence for the potential ulcer-preventive ability of phenolics in ginger aqueous extract and addresses the probable mode of action through the inhibition of gastric H + , K + -ATPase and Helicobacter pylori growth (Siddaraju et al, 2007;Nanjundaiah et al, 2009). Thus, ginger and many of its chemical constituents have been shown, in numerous experimental and clinical studies, to be useful in combating several health disturbances, providing evidences to explain the actions of folk medicines in terms of conventional biochemistry and pharmacology.…”

A novel phenol-bound pectic polysaccharide fromDecalepis hamiltoniiwith multi-step ulcer preventive activity

“…Determination of the phenolic content and antioxidant activity of SRPP Since phenolics are generally found to be associated with polysaccharides, we evaluated the phenolic content in SRPP using a Folin-Ciocalteu reagent as described previously [16] . Gallic acid was used as a standard for the generation of a calibration curve.…”

Impact of ginger aqueous extract on carbimazole induced testicular degenerative alterations and oxidative stress in albino rats

“…Superoxide dismutase, ascorbic acid, glutathione and uric acid levels were reduced and xanthine oxidase, glutathione-s-transferase activities were increased in alcohol treated rats while these parameters came to normalcy in ethanolic extract of ginger treated rats demonstrating the antioxidant effect of ginger [44]. Siddaraju and Dharmesh [45] demonstrated that ginger-free phenolic and ginger hydrolysed phenolic divisions showed free radical scavenging, inhibition of lipid peroxidation, protection of DNA and diminishing power abilities indicating strong antioxidant properties. Ghasemzadeh et al [46] reported that young rhizome of Z. officinale had higher content of flavonoids with high antioxidant activity.…”