Inhibition of Gene Expression in<i>Escherichia coli</i>under Hypergravity

Shimoshige, Hirokazu; Kobayashi, Hideki; Usami, Ron

doi:10.1271/bbb.100671

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…OmpW is involved in diverse biological processes including the response against environmental stress (41), resistance to antibiotics (42–44), and has been reported to contribute to bacterial virulence (45, 46). Li and colleagues have demonstrated that OmpW is upregulated when bacteria are incubated in the presence of normal human serum (40).…”

Section: Complement Evasion Strategies By E Colimentioning

confidence: 99%

How Escherichia coli Circumvent Complement-Mediated Killing

Abreu

Barbosa

2017

Front. Immunol.

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Complement is a crucial arm of the innate immune response against invading bacterial pathogens, and one of its main functions is to recognize and destroy target cells. Similar to other pathogens, Escherichia coli has evolved mechanisms to overcome complement activation. It is well known that capsular polysaccharide may confer resistance to complement-mediated killing and phagocytosis, being one of the strategies adopted by this bacterium to survive in serum. In addition, proteases produced by E. coli have been shown to downregulate the complement system. Pic, an autotransporter secreted by different pathogens in the Enterobacteriaceae family, is able to cleave C2, C3/C3b, and C4/C4b and works synergistically with human Factor I and Factor H (FH), thereby promoting inactivation of C3b. Extracellular serine protease P, a serine protease of enterohemorrhagic E. coli (EHEC), downregulates complement activation by cleaving C3/C3b and C5. StcE, a metalloprotease secreted by EHEC, inhibits the classical complement-mediated cell lysis by potentiating the action of C1 inhibitor, and the periplasmic protease Prc contributes to E. coli complement evasion by interfering with the classical pathway activation and by preventing membrane attack complex deposition. Finally, it has been described that E. coli proteins interact with negative complement regulators to modulate complement activation. The functional consequences resulting from the interaction of outer membrane protein A, new lipoprotein I, outer membrane protein W, and Stx2 with proteins of the FH family and C4b-binding protein (C4BP) are discussed in detail. In brief, in this review, we focused on the different mechanisms used by pathogenic E. coli to circumvent complement attack, allowing these bacteria to promote a successful infection.

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Section: Complement Evasion Strategies By E Colimentioning

confidence: 99%

How Escherichia coli Circumvent Complement-Mediated Killing

Abreu

Barbosa

2017

Front. Immunol.

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“…With the advancement of science and technology, microorganisms are getting more attention. Related studies have shown that microgravity can affect microorganisms in many aspects, including growth, gene expression and metabolic response [8][9][10]. Previous studies have reported that hyper-gravity can directly affect Paramecium, which is single-celled organisms, and inhibit its proliferation even for 1.8×g [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Survival of soil microbial community exposed to hyper-gravity conditions

Xu¹,

Liu²,

Zhao³

et al. 2021

Preprint

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Earth is the cradle of mankind, but it is impossible for human beings to live in the cradle forever. Sending soil microbial spores through space to foreign planets will be a likely initial process in planet colonization. Periods of hyper-gravity are likely to be a challenge for the candidate microorganisms during their interstellar transportation, raising questions about their survival rates and community-level responses. To address these questions, the impacts of hyper-gravity on soil microbial community composition and activity were tested by applying 1×g or 2500×g centrifugal force to soil for 6 days. The results indicated an increased diversity and absolute abundance of soil total bacterial community and a relatively stable active bacterial community under hyper-gravity condition. Besides, hyper-gravity had no observable effect on the relative abundance of soil microorganisms. These results suggest that soil microorganisms could survive during short periods of hyper-gravity. Our findings represent the first step towards a better understanding of the potential for survival of soil microbiomes during space travel and provide a basis for further interstellar soil research.

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Contribution of the outer membrane protein OmpW in Escherichia coli to complement resistance from binding to factor H

Wen

et al. 2016

Microbial Pathogenesis

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Inhibition of Gene Expression inEscherichia coliunder Hypergravity

Cited by 3 publications

References 15 publications

How Escherichia coli Circumvent Complement-Mediated Killing

How Escherichia coli Circumvent Complement-Mediated Killing

Survival of soil microbial community exposed to hyper-gravity conditions

Contribution of the outer membrane protein OmpW in Escherichia coli to complement resistance from binding to factor H

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