Inhibition of HDAC3 and ATXN3 by miR-25 prevents neuronal loss and ameliorates neurological recovery in cerebral stroke experimental rats

Zhou, Xiaomei; Qiao, Benyu

doi:10.1007/s13105-021-00848-3

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…MiR-25 was reported to be involved in epilepsy, Parkinson's disease, cerebral ischemia, multiple sclerosis, panic disorder, alexithymia, and tic disorders (Guo et al, 2014;Zhang et al, 2016;Nuzziello et al, 2018;Min et al, 2019;Van der Auwera et al, 2022; Wang et al, 2022;Zhou and Qiao, 2022). The present study demonstrated that miR-25 might also be associated with schizophrenia, further confirming the role of miR-25 in neuropsychiatric diseases and suggesting that miR-25-3p might be a potential therapeutic target or a diagnosis biomarker of schizophrenia.…”

Section: Discussionsupporting

confidence: 81%

“…Previous studies showed that miR-25 is involved in the pathogenesis of various diseases, such as acute myocardial infarction, left ventricular hypertrophy, heart failure, diabetes mellitus, diabetic nephropathy, tubulointerstitial nephropathy, and asthma bronchiale (Sárközy et al, 2018). Moreover, miR-25 was found to play a critical role in the pathogenesis of a range of neuropsychiatric diseases, including epilepsy (Li et al, 2020), Parkinson's disease (Chen et al, 2020), cerebral ischemia (Guo et al, 2014;Zhang et al, 2016;Zhou and Qiao, 2022), multiple sclerosis (Nuzziello et al, 2018), panic disorder (Min et al, 2019), alexithymia (Van der Auwera et al, 2022), and tic disorders (Wang et al, 2022). It was also reported that sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA2), a downstream target of miR-25, was elevated in the brains of patients with schizophrenia (Earls et al, 2012), suggesting a potential connection between miR-25 and schizophrenia.…”

Section: Introductionmentioning

confidence: 99%

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The SIK1/CRTC2/CREB1 and TWIST1/PI3K/Akt/GSK3β signaling pathways mediated by microRNA-25-3p are altered in the schizophrenic rat brain

Pan

Zhu²,

Han³

et al. 2023

Front. Cell. Neurosci.

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Schizophrenia is a group of severe mental disorders. MiR-25-3p was shown to be involved in various neuropsychiatric diseases and can regulate SIK1 and TWIST1. The CRTC2/CREB1 and PI3K/Akt/GSK3β signaling pathways are downstream pathways of SIK1 and TWIST1, respectively. This study investigated whether miR-25-3p-mediated SIK1/CRTC2/CREB1 and TWIST1/PI3K/Akt/GSK3β signaling pathways are present in an animal model relevant to schizophrenia. A schizophrenic rat model was established by using sub-chronic MK-801 administration. An RNA-seq test was performed to examine the differentially expressed genes (DEGs) in the rat prefrontal cortex (PFC). The mRNA levels of miR-25-3p, SIK1, and TWIST in the PFC and caudate putamen (CPu) were assessed by qRT-PCR. Phosphorylation of the SIK1/CRTC2/CREB1 and TWIST1/PI3K/Akt/GSK3β pathways in the two brain regions was examined by Western blots. The RNA-seq data revealed down-regulated miR-25-3p expression and up-regulated SIK1 and TWIST1 mRNA expression induced by MK-801. Additionally, SIK1 and TWIST1 were shown to be possible downstream responders of miR-25-3p in previous studies. qRT-PCR confirmed the changes of miR-25-3p, SIK1, and TWIST1 induced by MK-801 in both brain regions, which, however, was reversed by risperidone. Furthermore, the phosphorylation of the SIK1/CRTC2/CREB1 pathway was repressed by MK-801, whereas the phosphorylation of the TWIST1/PI3K/Akt/GSK3β pathway was increased by MK-801 in either of the two brain regions. Moreover, the altered phosphorylation of these two signaling pathways induced by MK-801 can be restored by risperidone. In conclusion, this study suggests that altered SIK1/CRTC2/CREB1 and TWIST1/PI3K/Akt/GSK3β signaling pathways mediated by miR-25-3p is very likely to be associated with schizophrenia, revealing potential targets for the treatment and clinical diagnosis of schizophrenia.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

The SIK1/CRTC2/CREB1 and TWIST1/PI3K/Akt/GSK3β signaling pathways mediated by microRNA-25-3p are altered in the schizophrenic rat brain

Pan

Zhu²,

Han³

et al. 2023

Front. Cell. Neurosci.

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“…It has been shown that miR-25 levels in the peripheral blood of ACI patients are considerably lower than those of healthy controls [ 26 ]. According to findings from other basic investigations, miR-25 plays a protective role in cerebral ischemia-reperfusion injury [ 27 ]. miR-182 can aggravate brain injury by downregulating the expression of anti-apoptotic proteins and regulating the oxidative stress process, which is directly associated with the development and occurrence of cerebral infarction; and its relative expression level is significantly increased in the peripheral blood of ACI patients [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Fuyuan Xingshen Decoction Combined with Butylphthalide Sodium Chloride Injection in the Treatment of Acute Cerebral Infarction and Its Effect on Hemodynamics

Zhang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. This study aims to determine the curative effect of Fuyuan Xingshen Decoction combined with butylphthalide sodium chloride injection in acute cerebral infarction (ACI) treatment and its effect on hemodynamics. Methods. In our hospital, a total of 84 ACI patients from May 2020 to February 2022 were randomly divided into two groups: observation and control (n = 42 each). Fuyuan Xingshen Decoction in combination with butylphthalide sodium chloride injection was provided to the observation group, while the control group received butylphthalide sodium chloride injection alone. Both groups’ clinical efficacy was observed. Before and after treatment, the neurological function of the two groups was evaluated using the National Institutes of Health Stroke Scale (NIHSS), while the daily living ability of both groups was determined using the Barthel index rating scale. Both groups were investigated for their cognitive function, before and after treatment, by using the Mini-Cog scale and Montreal Cognitive Assessment (MoCA) scale. The pulsatility index (PI), peak velocity (Vp), and mean velocity (Vm) of intracranial arteries were measured by transcranial Doppler ultrasonography before and after treatment. The levels of ubiquitin carboxy-terminal hydrolase-1 (UCH-L1), Fibulin-5, and visinin-like protein-1 (VILIP-1) in serum and the expression levels of miR-34c, miR-25, and miR-182 in peripheral blood of the two groups were compared. Both groups were observed for the incidence of adverse reactions. Results. Compared with the control (71.43%), the observation group exhibited a significantly higher effective rate of around 90.48%. In the observation group, the improvement in NIHSS, Barthel index, Mini-cog, and MoCA scores; hemodynamic indexes including Vp, PI, and Vm; serum UCH-L1, Fibulin-5, and VILIP-1 levels; and the miR-34c, miR-25, and miR-182 expression levels in peripheral blood was better than the control group, with significant difference (all P < 0.05 ). The incidence of adverse reactions between the groups demonstrated no significant differences ( P > 0.05 ). Conclusions. Fuyuan Xingshen Decoction combined with butylphthalide sodium chloride injection can effectively improve patients’ daily living ability, neurological function, cognitive function, and cerebral hemodynamics in the treatment of ACI, with good effect and safety.

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“…It is possible that post-stroke non-stimulated miR-25 tissue levels could be too low at the different explored time points to become detectable in blood by the methodology used in our study. Another recent study confirmed the overexpression of miR-25 in the plasma of acute ischemic stroke patients and the ischemic brain tissue of rats subjected to MCAo and demonstrated a neuroprotective and neuroplasticity role for this miRNA [ 46 ].…”

Section: Discussionmentioning

confidence: 86%

Can Repetitive Transcranial Magnetic Stimulation (rTMS) Promote Neurogenesis and Axonogenesis in Subacute Human Ischemic Stroke?

De Michele,

Piscopo,

Costanzo

et al. 2024

Biomedicines

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Background: Ischemic stroke may trigger neuroplastic changes via proliferation, migration towards the lesion, and differentiation of neuroprogenitor cells into mature neurons. Repetitive Transcranial Magnetic Stimulation (rTMS) may promote brain plasticity. This study aimed to assess rTMS’s effect on post-stroke endogenous neuroplasticity by dosing plasma miRs 17~92, Netrin-1, Sema3A, and BDNF. Methods: In this case-controlled study, we randomized 19 ischemic stroke patients within five days from symptoms onset (T0) to neuronavigated-rTMS or sham stimulation. Stimulation was applied on the stroke hemisphere daily between the 7th and 14th day from stroke onset. Blood samples were collected at T0, before the first rTMS section (T7), and at the end of the last rTMS session (T14). Five healthy controls were also enrolled in this study. Results: Of 19 patients, 10 received rTMS and 9 sham stimulation. Compared with the sham group, in the rTMS group, plasma levels of miRs17~92 and Ntn-1 significantly increased whereas Sema3A levels tended to decrease. In multivariate linear regression analyses, rTMS was independently related to Ntn-1 and miR-25 levels at T14. Conclusions: We found an association between rTMS and neurogenesis/axonogenesis biomarker enhancement. Our preliminary data suggest that rTMS may positively interfere with natural endogenous plasticity phenomena of the post-ischemic human brain.

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Inhibition of HDAC3 and ATXN3 by miR-25 prevents neuronal loss and ameliorates neurological recovery in cerebral stroke experimental rats

Cited by 9 publications

References 18 publications

The SIK1/CRTC2/CREB1 and TWIST1/PI3K/Akt/GSK3β signaling pathways mediated by microRNA-25-3p are altered in the schizophrenic rat brain

The SIK1/CRTC2/CREB1 and TWIST1/PI3K/Akt/GSK3β signaling pathways mediated by microRNA-25-3p are altered in the schizophrenic rat brain

Efficacy of Fuyuan Xingshen Decoction Combined with Butylphthalide Sodium Chloride Injection in the Treatment of Acute Cerebral Infarction and Its Effect on Hemodynamics

Can Repetitive Transcranial Magnetic Stimulation (rTMS) Promote Neurogenesis and Axonogenesis in Subacute Human Ischemic Stroke?

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