Inhibition of hematopoietic stem cells by syngeneic lymphocytes

Kaulen, D.; Golovanova, T. A.; Pyatykhina, D P; Khorobrykh, V. V.

doi:10.1007/bf00809620

Cited by 1 publication

(2 citation statements)

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“…Our previous studies have shown that the decrease of mitochondrial membrane potential in oocytes can cause the release of cytochrome c, which in turn activates the up-regulation of intracellular apoptosis-related caspase and leads to apoptosis of oocytes. 27,58,59 To establish whether this same apoptotic mechanism also occurs in arecoline-exposed oocytes, the expression levels of oxidative stress and apoptosis-related genes and proteins further were validated. Our results clearly revealed that caspase signalling pathway-related genes and proteins Bax, Bak, Caspase9 and Caspase3 were significantly elevated compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

“…To further explore the specific mechanism of apoptosis caused by arecoline, we examined the mitochondrial membrane potential of oocytes, and the results showed that arecoline significantly reduced the membrane potential of mitochondria. Our previous studies have shown that the decrease of mitochondrial membrane potential in oocytes can cause the release of cytochrome c , which in turn activates the up‐regulation of intracellular apoptosis‐related caspase and leads to apoptosis of oocytes 27,58,59 . To establish whether this same apoptotic mechanism also occurs in arecoline‐exposed oocytes, the expression levels of oxidative stress and apoptosis‐related genes and proteins further were validated.…”

Section: Discussionmentioning

confidence: 99%

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Metformin protects against mouse oocyte apoptosis defects induced by arecoline

Zang

Yin

et al. 2020

Cell Proliferation

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Objectives Arecoline is the main bioactive substance extracted from Areca catechu L, which has cell, neural and genetic toxicity. The function of arecoline in reproductive system has not been well explored. Materials and Methods To investigate the toxic effects of arecoline on oocyte development, immunofluorescence staining, qPCR, Western blotting, sperm binding assays and in vitro fertilization were performed to evaluate oocyte meiosis competence and embryo development. Results Our data revealed that arecoline exposure disrupts actin filament dynamics, spindle assembly and kinetochore‐microtubule attachment stability in mouse oocytes, leading to aneuploidy and oocyte meiosis arrest. In addition, arecoline treatment disturbs the distribution of mitochondria, reduces ATP production and increases the level of oxidative stress, which ultimately induces oocyte apoptosis. Supplementation with metformin, a medicine for type 2 diabetes in the clinic, partially alleviates these damages. Conclusions Metformin has a protective effect on arecoline‐induced mouse oocytes apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%