Inhibition of homodimerization of Toll-like receptor 4 by curcumin

Youn, Hyung S.; Saitoh, Shu‐ichi; Miyake, Kensuke; Hwang, Daniel

doi:10.1016/j.bcp.2006.03.022

Cited by 208 publications

(172 citation statements)

References 37 publications

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“…Recent studies of natural products with anti-inflammatory activity have been reported, and many of them had been done using macrophage-like cell lines, RAW264.7 cells 8,10,16,[26][27][28] and THP-1 cells. 11,13) However, we chose human colon carcinoma cells for screening because we were focusing on anti-inflammatory compounds in dietary products which might be effective through the mucosal immune response.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice

Ishii

Katsumura

Shiozuka

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice

Ishii

Katsumura

Shiozuka

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…Both procedures were performed as described previously. 24,25) RAW 264.7 or 293T cells were cotransfected with NF-B-luciferase plasmid and HSP70--galactosidase plasmid as an internal control using SuperFect transfection reagent (Qiagen) according to the manufacturer's instructions. Various expression plasmids or the corresponding empty vector plasmids for signaling components were co-transfected.…”

Section: Methodsmentioning

confidence: 99%

TBK1-Targeted Suppression of TRIF-Dependent Signaling Pathway of Toll-Like Receptors by 6-Shogaol, an Active Component of Ginger

Park

Lee

Son

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…We next determined whether auranofin inhibits LPS-induced dimerization of TLR4 using IL-3-dependent Ba/F3 cells stably transfected with murine TLR4-Flag and TLR4-GFP, MD2, CD14, and NF-κB-luciferase reporter gene as described previously [19,22]. Auranofin inhibited the dimerization of TLR4 ( Fig.…”

Section: Lps-induced Dimerization Of Tlr4 Was Inhibited By Gold Compomentioning

confidence: 99%

Auranofin, as an anti-rheumatic gold compound, suppresses LPS-induced homodimerization of TLR4

Youn

Lee

Saitoh

et al. 2006

Biochemical and Biophysical Research Communications

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Toll-like receptors (TLRs), which are activated by invading microorganisms or endogenous molecules, evoke immune and inflammatory responses. TLR activation is closely linked to the development of many chronic inflammatory diseases including rheumatoid arthritis. Auranofin, an Au(I) compound, is a well-known and long-used anti-rheumatic drug. However, the mechanism as to how auranofin relieves the symptom of rheumatoid arthritis has not been fully clarified. Our results demonstrated that auranofin suppressed TLR4-mediated activation of transcription factors, NF-κB and IRF3 and expression of COX-2, a pro-inflammatory enzyme. This suppression was well correlated with the inhibitory effect of auranofin on the homodimerization of TLR4 induced by an agonist. Furthermore, auranofin inhibited NF-κ activation induced by MyD88-dependent downstream signaling components of TLR4, MyD88, IKKβ, and p65. IRF3 activation induced by MyD88-independent signaling components, TRIF and TBK1, was also downregulated by auranofin. Our results first demonstrate that auranofin suppresses the multiple steps in TLR4 signaling, especially the homodimerization of TLR4. The results suggest that the suppression of TLR4 activity by auranofin may be the molecular mechanism through which auranofin exerts anti-rheumatic activity.

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Inhibition of homodimerization of Toll-like receptor 4 by curcumin

Cited by 208 publications

References 37 publications

Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice

Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice

TBK1-Targeted Suppression of TRIF-Dependent Signaling Pathway of Toll-Like Receptors by 6-Shogaol, an Active Component of Ginger

Auranofin, as an anti-rheumatic gold compound, suppresses LPS-induced homodimerization of TLR4

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