Inhibition of human gastric carcinoma cell growth in vitro by a polysaccharide from Aster tataricus

Zhang, Yunxin; Wang, Qiusheng; Wang, Tie; Zhang, Haikui; Tian, Ying; Luo, Hong; Shen, Yang; Wang, Yuan; Huang, Xun

doi:10.1016/j.ijbiomac.2012.06.019

Cited by 30 publications

(11 citation statements)

References 19 publications

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“…The medium was replaced with dimethyl sulfoxide to dissolve the formazan produced from MTT by viable cells. Absorbance at 450 nm is proportional to the number of live cells [22] . Cell viability, expressed as the absorbance of melittin-treated cells, is reported relative to PBStreated controls.…”

Section: Growth Inhibition Assaymentioning

confidence: 99%

Melittin induces human gastric cancer cell apoptosisviaactivation of mitochondrial pathway

Kong¹,

Wenhua²,

Diao³

et al. 2016

WJG

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Section: Growth Inhibition Assaymentioning

confidence: 99%

Melittin induces human gastric cancer cell apoptosisviaactivation of mitochondrial pathway

Kong¹,

Wenhua²,

Diao³

et al. 2016

WJG

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“…chromatograms of protocatechuic acid (1); chlorogenic acid (2); caffeic acid (3); scopoletin (4); 7-hydroxycoumarin (5); ferulic acid (6); isoquercitrin (7); kaempferol-7-O-β-D-glucopyranoside (8); methyl caffeate (9); quercetin (10); luteolin (11); kaempferol (12); isorhamnetin (13); shionone (14); epifriedelinol (15).…”

Section: F I G U R E 2 Multiple Reaction Monitoring (Mrm)mentioning

confidence: 99%

Comparison of the active components of Aster tataricus from different regions and related processed products by ultra‐high performance liquid chromatography with tandem mass spectrometry

Yao

Huang

et al. 2020

J of Separation Science

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We investigated crude Aster tataricus, vinegar‐processed Aster tataricus, honey‐processed Aster tataricus, and steamed Aster tataricus as a case study and developed a comprehensive strategy integrating quantitative analysis and chemical pattern recognition methods for the evaluation and differentiation of Aster tataricus from different regions, as well as related processed products. In the study, 15 batches of raw Aster tataricus collected from seven provinces were analyzed. A sensitive and rapid ultra‐high performance liquid chromatography with tandem mass spectrometry method for simultaneous determination of 15 compounds was established to evaluate the quality of raw and processed Aster tataricus. Furthermore, multivariate statistical techniques were applied to compare the differences among Aster tataricus samples. As a result, the herbs collected from seven provinces were divided into two categories, and chlorogenic acid was the most important component distinguishing between the regions. Moreover, all of the raw and processed samples were classified by partial least squares discriminant analysis based on the 15 analyzed compounds. Results showed that raw Aster tataricus, vinegar‐processed Aster tataricus, honey‐processed Aster tataricus, and steamed Aster tataricus were clustered in four different areas. Shionone, chlorogenic acid and kaempferol were the significant constituents differentiating the raw and differently processed Aster tataricus samples.

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“…Aster tataricus Linn., from the family Asteraceae, is distributed widely in Asian countries and has been used as a common traditional medicine for the relief of coughs and as an expectorant. Scientific studies revealed that A. tataricus possessed diuretic, antitumor, antibacterial, antivirus, antiulcer activities and related hepatic microsomal biotransformation [14,15] . The major constituents of A. tataricus are terpenoids, sterols, flavonoids, polysaccharides and cylclopeptides [16][17] .…”

mentioning

confidence: 99%

“…Scientific studies revealed that A. tataricus possessed diuretic, antitumor, antibacterial, antivirus, antiulcer activities and related hepatic microsomal biotransformation [14,15] . The major constituents of A. tataricus are terpenoids, sterols, flavonoids, polysaccharides and cylclopeptides [16][17] . Recently, the roots and rhizomes of A. tataricus have been reported to inhibit HBV DNA replications and the human gastric carcinoma cell growth indicating that it could act as a natural anticancer agent [16,17] .…”

mentioning

confidence: 99%

“…The major constituents of A. tataricus are terpenoids, sterols, flavonoids, polysaccharides and cylclopeptides [16][17] . Recently, the roots and rhizomes of A. tataricus have been reported to inhibit HBV DNA replications and the human gastric carcinoma cell growth indicating that it could act as a natural anticancer agent [16,17] . A. tataricus was also reported to have antioxidant activity and reduce the levels of alanine aminotransferase (ALS), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in hepatotoxic effect induced by Fr-2 [18,19] .…”

mentioning

confidence: 99%

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Aster tataricus Linn., Suppresses Hepatic Stellate Cell Activation and Protects Against Thioacetamide-induced Liver Fibrosis in Rats

Kim¹,

Koppula²,

Yum³

et al. 2020

ijps

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Kim et al.: Antihepatofibrotic Effect of Aster tataricusAster tataricus Linn., (Asteraceae), an oriental and nutrient-potential herb is used in Asian countries for various health benefits. The present study focused on the protective effects of Aster tataricus against liver fibrosis in cellular and an experimental rat model. Cell cytotoxicity, cell cycle and apoptosis functions were analyzed using hepatic stellate cell lines following MTT assay, flow cytometry, and Annexin V-FITC/PI staining methods. For in vivo evaluation, thioacetamide-induced hepatofibrosis rat model was established. Sprague Dawley rats were divided into 5 groups of 10 each (control, thioacetamide, thioacetamide with Aster tataricus extract 100 and 500 mg/kg and silymarin 50 mg/kg groups, respectively). Fibrosis was induced by thioacetamide treatment (200 mg/kg, ip) 3 times per week for 13 weeks except for the control group. Aster tataricus extract (100 and 500 mg/kg), and silymarin was administrated orally to each group 6 times a week from week 7 to 13 and various fibrosis-related parameters were estimated using real-time polymerase chain reaction using TRIzol Plus RNA purification Kit and a spectrophotometer. Results indicated that hepatic stellate cells treated with Aster tataricus extract (0.5 mg/ml) and silymarin (0.05 mg/ml) significantly (p<0.05) induced apoptosis (19.04 and 24.82 %, respectively) compared to the control group (9.78 %). Moreover, rat primary hepatic stellate cells showed morphological changes and degradation of collagen and fibronectin when treated with 0.5 mg/kg of Aster tataricus extract. In vivo evaluation Aster tataricus extract at concentrations of 100 and 500 mg/mlattenuated the increased serum levels of alanine transaminase, aspartate transaminase and hydroxyproline and restored the decreased glutathione levels significantly in thioacetamide induced fibrotic rats (p<0.05). The altered histopathology in thioacetamide-induced liver tissues and changes in fibrosis-related gene expression (TGF-β, α-SMA and Col1α1) were also restored by Aster tataricus extract treatment. In conclusion, Aster tataricus can be developed as a potential therapeutic agent in the treatment of liver fibrosis.

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Inhibition of human gastric carcinoma cell growth in vitro by a polysaccharide from Aster tataricus

Cited by 30 publications

References 19 publications

Melittin induces human gastric cancer cell apoptosisviaactivation of mitochondrial pathway

Melittin induces human gastric cancer cell apoptosisviaactivation of mitochondrial pathway

Comparison of the active components of Aster tataricus from different regions and related processed products by ultra‐high performance liquid chromatography with tandem mass spectrometry

Aster tataricus Linn., Suppresses Hepatic Stellate Cell Activation and Protects Against Thioacetamide-induced Liver Fibrosis in Rats

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