Inhibition of human leukocyte migration in agarose by KCl extracts of carcinoma of the lung

Boddie, Arthur W.; Holmes, E. Carmack; Roth, Jack A.; Morton, Donald L.

doi:10.1002/ijc.2910150514

Cited by 45 publications

(17 citation statements)

References 21 publications

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“…Lymphocytes reacted most frequently with the targets cultured from lung tumours in cytotoxicity tests (Hellstrom et al, 1971;Baldwin et al, 1973;Vose et al, 1975;Pierce and De Vald, 1975) or with lung homogenates in leukocyte migration inhibition assays (Boddie et al, 1975;Vose et al, 1977a). Reactions in skin testing with tumour homogenates also showed organ-related reactivity (Hollinshead et al, 1974).…”

Section: Discussionmentioning

confidence: 98%

“…The implication of these studies is that tumours from the lung share common antigenic specificities not present on neoplasms arising at other sites. Reactivity against apparently non-malignant lung tissue in migration inhibition assays (Vose et at., 1975(Vose et at., , 1977aBoddie et al, 1975), the involvement of NK reactions and effector functions of non-T lymphocytes (Vose and Moore, 1977) against cultured allogeneic targets in cytotoxicity tests, may to some extent obscure this interpretation. However, the apparent autologous reactivity could arise from histocompatibility restriction of cytotoxicity to a common lung-tumour-associated antigen.…”

Section: Discussionmentioning

confidence: 99%

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Restricted autologous lymphocytotoxicity in lung neoplasia

Vose¹,

Vánky²,

Fopp³

et al. 1978

Br J Cancer

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Summary.-Blood lymphocytes from 47 patients with lung carcinoma have been tested for cytotoxicity against cells isolated from the autologous tumour. Significant cytotoxic potential was found in 15 cases. The effectors were also tested against allogeneic tumour targets from lung and other sites. Reactions were only rarely detected (2/32 positive against lung and 1/13 positive against non-lung cells). The restriction of cytotoxicity to the autologous combination was also apparent in in vitro-generated effectors. Blood lymphocytes were co-cultivated with autologous tumour and subsequently tested against autologous or allogeneic targets. Cytotoxicity was found in 13/17 lung tumours against autologous tumour, with no reactions recorded against allogeneic tumour targets, but one case positive against the K562 cell line. These data suggest either the expression of individually distinct antigens on human pulmonary neoplasms, or the requirement for histocompatibility between target and effector in cytotoxicity reactions in man, and therefore differ from previously described patterns of lymphocytotoxicity against human tumours.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Restricted autologous lymphocytotoxicity in lung neoplasia

Vose¹,

Vánky²,

Fopp³

et al. 1978

Br J Cancer

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“…Such data emphasizes that different modalities of membrane solubilization may yield different TAA. These data serve to suggest that lung TAMA-1 is not likely to have been present in biologically significant amounts in 3M KCI extracts tested by several researchers (Boddie et al, 1975;Dean et al, 1978;McCoy et al, 1977;Pierce & DeVald, 1975;Roth et al, 1975). However, other reports by Hollinshead et al (1974Hollinshead et al ( , 1975 are more likely to have contained lung TAMA-I.…”

Section: Discussionmentioning

confidence: 70%

“…Other investigators, using more crude lung TAMA generated by 3M KCI solubilization, have demonstrated cell-mediated immune (CMI) reactivity of lung-cancer patients to such preparations. The in vitro CMI methods have included lymphocyte blastogenesis Dean et al, 1978), leucocyte-migration inhibition (Boddie et al, 1975;Cannon et al, 1977;McCoy et al, 1977) as well as microcytotoxicity (Pierce & DeVald, 1975).…”

Section: Discussionmentioning

confidence: 99%

A human tumour-associated membrane antigen from squamous-cell carcinoma of the lung

Veltri¹,

Maxim²,

Boehlecke³

1980

Br J Cancer

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Summary.-Primary human squamous-cell lung tumours were disrupted using mechanical high-speed homogenization. Crude membranes were isolated by differential centrifugation at low speeds followed by 100,000 g centrifugation. Such membrane preparations were extracted with Triton-X-100 and passed over a DEAE cellulose column; the DEAE unbound fraction and the bound fraction eluted with 0-4M NaCl were used to immunize rabbits. We present here only data on a single lung-tumour-associated membrane antigen (TAMA) found in the unbound DEAE cellulose column eluates. This new Triton-X-100 extractable antigen is termed lung TAMA-1.A further purification of the antigen was achieved using two methods. The first used G-200 molecular-seive chromatography and this revealed lung TAMA-1 to have a mol. wt of 200,000. A second method used sucrose density-gradient isoelectric focusing, and the antigen had an isoelectric point of -3 0.Several important properties of the lung TAMA-1 were determined. The antigen has a cathodal gamma-type electrophoretic mobility at pH 7-6. The antigen was not detected in any normal human adult or foetal tissue extracts tested. It did not crossreact immunochemically with CEA, AFP or p2 microglobulin. Lung TAMA-1 was detected in 80% of lung tumour Triton-X-100 extracts tested by counterimmunoelectrophoresis (CIEP) but was undetectable in breast or colon carcinoma extracts.Low frequency sonication did not deleteriously affect lung TAMA-1, but, 3M KCI eliminated its immunologic reactivity in CIEP. Finally, preliminary data were obtained using immunohistochemistry to localize in vivo lung TAMA-1 production.

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“…RECENTLY, several investigators have reported that specific cellular anti-tumour reactivity can be detected by means of the leucocyte migration inhibition (LMI) test from capillary tubes (Cochran et al, 1973;Kjaer,1975;McCoy et al, 1975) and also by migration under agarose (Bergstrand et al, 1974;Boddie et al, 1975;Tautz et al, 1974). The purpose of the present study was to compare the macrophage electrophoretic mobility (MEM) test introduced by Caspary and Field (1970) with LMI under agarose (Clausen, 1971) using allogeneic KCI extracts of tumours and normal tissues, carcinoembryonic antigen (CEA) and human encephalitogenic protein (HEP).…”

mentioning

confidence: 99%