Inhibition of Human T Lymphocyte Proliferation in vitro by Commercial Factor VIII Concentrates

Wang, Y.; Beck, Eugene A.; Furlan, Miha; Weck, A.L. de

doi:10.1111/j.1423-0410.1985.tb00194.x

Cited by 7 publications

(1 citation statement)

References 11 publications

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“…Numerous phenotypic and functional abnormalities of the immune system have been described in haemophilic patients that are unrelated to HIV infection, including quantitative abnormalities of lymphocytes (Antonaci et al, 1987), functional T lymphocyte abnormalities (Mahir et al, 1988) and decreased natural killer activity (Matheson et al, 1986). Early evidence for the direct action of FVIII concentrates as immune modulators was provided by the observation that lymphocyte proliferation and interleukin-2 (IL-2) production could be reduced in vitro by FVIII concentrate (Wang et al 1985;Lederman et al, 1986). Similarly, Eibl and her colleagues demonstrated that rnonocyte function (Fc receptor expression, Fc receptor mediated phagocytosis) could be inhibited in vitro by FVIII products (Eibl et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo inhibition of monocyte phagocytic function by factor VIII concentrates: correlation with concentrate purity

Pasi

Hill

1990

Br J Haematol

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Seven factor VIII concentrates of varying purity (specific activity 0.67-10 units factor VIII procoagulant activity (VIII: C)/mg protein) have been examined over a range of concentrations (0.5-0.005 u/ml factor VIII) to establish their effect on in vitro monocyte phagocytic function. All these products inhibited monocyte phagocytic function, but the monoclonally purified product tested was significantly less inhibitory than the others (P less than 0.05). The degree of inhibition observed was independent of the virus inactivation method used to increase product safety. However, comparison of these products of differing purity from the same manufacturer showed that the inhibitory effect decreased as product purity increased. In vivo studies of monocyte function following infusion of two of these factor VIII concentrates demonstrated inhibition of circulating monocytes. The possible role of product purity in modulating immune responses in haemophiliacs is discussed.

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo inhibition of monocyte phagocytic function by factor VIII concentrates: correlation with concentrate purity

Pasi

Hill

1990

Br J Haematol

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Lymphocyte responses to human cytomegalovirus in different groups of patients in Britain and in adults from West Africa and the Middle East

et al. 1996

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Antibody prevalence and lymphocyte proliferation responses to cytomegalovirus (CMV) and herpes simplex virus (HSV) were compared in several different groups of patients: genitourinary medical (GUM) patients, hemophiliacs, men with clinical acquired immunodeficiency syndrome (AIDS) and cases of primary CMV mononucleosis, and also in adults in the general population (control subjects) comprising separate groups native to Britain, West Africa, and the Middle East. Among the British control subjects who were positive for CMV IgG, all were also positive against CMV antigen by the lymphocyte transformation test (LTT). However, among those who were CMV IgG-positive in the various groups of patients, 20-86.9% gave positive responses to CMV antigen by the LTT; moreover, 75.7% and 55.5% of the CMV IgG-positive healthy control subjects from West Africa and the Middle East, respectively, gave positive LTT responses to CMV antigen. When the same groups of patients were tested for responsiveness to HSV antigen by the LTT, there was good agreement between a positive result by this test and by serology in all except those with primary CMV mononucleosis (42.8%). Overall, lymphocyte responses to CMV were significantly impaired in healthy, CMV antibody-positive subjects from West Africa and the Middle East compared to similar subjects from Britain.

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Immunoglobulin levels in haemophiliacs at HIV seroconversion and during follow up

et al. 1987

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Immunoglobulins and aminotransferases were followed in 66 haemophilia patients and 13 von Willebrand patients over a six-year period. The results were correlated to HIV serology and lymphocyte subsets. Elevated IgG levels were found in 29/53 patients with haemophilia A, 2/13 with haemophilia B and in 0/13 with von Willebrand's disease. Elevated IgA and IgM levels were seen in 20% and 27% of the patients respectively, with a distribution similar to the elevated IgG levels, except that elevated IgA and IgM levels were also seen in 4/13 patients with von Willebrand's disease. Patients with HIV antibodies had significantly higher immunoglobulin levels than seronegative patients, and this elevation occurred in connection with seroconversion in the majority of the former. The IgG levels could not be correlated to the T4 cell count, but there has been a trend to less clinical symptoms related to HIV infection among those with stable IgG levels during the past few years. No correlation was found between elevated IgG levels and the aminotransferase levels, nor was any correlation found with the amount of blood coagulation factor concentrate given to the patients. The elevation of immunoglobulins observed in our haemophiliacs is multifactorial, but HIV infection is maybe the most important mechanism. The longitudinal IgG pattern may contribute to the prediction of the clinical outcome of this infection.

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Inhibition of Human T Lymphocyte Proliferation in vitro by Commercial Factor VIII Concentrates

Cited by 7 publications

References 11 publications

In vitro and in vivo inhibition of monocyte phagocytic function by factor VIII concentrates: correlation with concentrate purity

In vitro and in vivo inhibition of monocyte phagocytic function by factor VIII concentrates: correlation with concentrate purity

Lymphocyte responses to human cytomegalovirus in different groups of patients in Britain and in adults from West Africa and the Middle East

Immunoglobulin levels in haemophiliacs at HIV seroconversion and during follow up

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