Inhibition of
 Neisseria gonorrhoeae
 by
 Lactobacillus
 Species That Are Commonly Isolated from the Female Genital Tract

Amant, Diane C. St.; Valentin-Bon, Iris E.; Jerse, Ann E.

doi:10.1128/iai.70.12.7169-7171.2002

Cited by 65 publications

(55 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NmB growth was also inhibited by L. plantarum but only in planktonic culture. It was not observed in agar overlays, in contrast to other studies of intestinal pathogens [50] and Neisseria gonnorhoea [51], suggesting phase-dependent inhibition of NmB.…”

Section: Adhesioncontrasting

confidence: 51%

Oral Lactobacillus plantarum NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of Neisseria meningitidis Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Bekele¹,

Keith²,

Adelina³

et al. 2014

AiM

View full text Add to dashboard Cite

In this study, we investigate the potential for oral Lactobacilli (LB) to afford innate protection against nasopharyngeal coloniser Neisseria meningitidis serogroup B (NmB), which causes the bulk of UK meningococcal disease. Oral isolates of L. plantarum, L. salivarious, L. casei, L. rhamnosus, L. gasseri and gut probiotic L. rhamnosus GG were assessed for their ability to suppress nasopharyngeal epithelial inflammatory responses to pathogenic NmB. The specificity of attenuation was examined using TLR 2 ligand, Pam3Cys, and early response cytokine IL1β; and the mechanism of attenuation was explored using heat-killed organisms and conditioned medium. Pro-inflammatory IL-6 and TNFα cytokine secretion was quantified by ELISA and associated cell death was quantified by PI staining and LDH release. NmB adhesion, invasion and metabolism were determined using standard gentamicin protection with viable counts, and bioluminescence, respectively. L. plantarum and L. salivarious suppressed IL-6 and TNFα secretions from NmB-infected epithelial cells. LB did not need to be alive and could suppress using secretions, which were independent of TLR2 or IL1β receptor signalling. L. plantarum, in particular, reduced NmB-induced necrotic cell death of epithelial monolayers. Like L. salivarious, it significantly inhibited NmB adhesion but uniquely L. plantarum abolished NmB invasion. Using bioluminescence as a reporter of pathogen metabolism, L. plantarum and its secretions were found to inhibit NmB metabolism during cell invasion assays. We conclude that oral L. plantarum and its secretions could be used to help reduce the burden of meningococcal disease by removing the intracellular nasopharyngeal reservoir of NmB.

show abstract

Section: Adhesioncontrasting

confidence: 51%

Oral Lactobacillus plantarum NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of Neisseria meningitidis Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Bekele¹,

Keith²,

Adelina³

et al. 2014

AiM

View full text Add to dashboard Cite

show abstract

“…Lactobacillus species have also been shown to inhibit gonococcal growth measured as zonal growth inhibition on agar plates [28].…”

Section: Introductionmentioning

confidence: 99%

Adherence of clinically isolated lactobacilli to human cervical cells in competition with Neisseria gonorrhoeae

Vielfort

Sjölinder

Roos

et al. 2008

Microbes and Infection

View full text Add to dashboard Cite

“…The ability of N. gonorrhoeae to survive oxidative damage is illustrated by its ability to survive among neutrophils (31,42,45). During infection, N. gonorrhoeae is also likely to encounter H 2 O 2 produced by commensal lactobacilli, which inhibit the growth of N. gonorrhoeae in vitro (61,49). Since N. gonorrhoeae is an obligate human pathogen, it is not exposed to typical environmental stresses such as UV light, ionizing radiation, or chemical mutagens.…”

mentioning

confidence: 99%

Neisseria gonorrhoeae DNA Recombination and Repair Enzymes Protect against Oxidative Damage Caused by Hydrogen Peroxide

Stohl

Seifert

2006

J Bacteriol

View full text Add to dashboard Cite

The strict human pathogen Neisseria gonorrhoeae is exposed to oxidative damage during infection. N. gonorrhoeae has many defenses that have been demonstrated to counteract oxidative damage. However, recN is the only DNA repair and recombination gene upregulated in response to hydrogen peroxide (H 2 O 2 ) by microarray analysis and subsequently shown to be important for oxidative damage protection. We therefore tested the importance of RecA and DNA recombination and repair enzymes in conferring resistance to H 2 O 2 damage. recA mutants, as well as RecBCD (recB, recC, and recD) and RecF-like pathway mutants (recJ, recO, and recQ), all showed decreased resistance to H 2 O 2 . Holliday junction processing mutants (ruvA, ruvC, and recG) showed decreased resistance to H 2 O 2 resistance as well. Finally, we show that RecA protein levels did not increase as a result of H 2 O 2 treatment. We propose that RecA, recombinational DNA repair, and branch migration are all important for H 2 O 2 resistance in N. gonorrhoeae but that constitutive levels of these enzymes are sufficient for providing protection against oxidative damage by H 2 O 2 .

show abstract

Inhibition of Neisseria gonorrhoeae by Lactobacillus Species That Are Commonly Isolated from the Female Genital Tract

Cited by 65 publications

References 18 publications

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Adherence of clinically isolated lactobacilli to human cervical cells in competition with Neisseria gonorrhoeae

Neisseria gonorrhoeae DNA Recombination and Repair Enzymes Protect against Oxidative Damage Caused by Hydrogen Peroxide

Contact Info

Product

Resources

About

Inhibition of Neisseria gonorrhoeae by Lactobacillus Species That Are Commonly Isolated from the Female Genital Tract

Cited by 65 publications

References 18 publications

Oral &lt;i&gt;Lactobacillus plantarum&lt;/i&gt; NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of &lt;i&gt;Neisseria meningitidis&lt;/i&gt; Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Oral &lt;i&gt;Lactobacillus plantarum&lt;/i&gt; NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of &lt;i&gt;Neisseria meningitidis&lt;/i&gt; Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Adherence of clinically isolated lactobacilli to human cervical cells in competition with Neisseria gonorrhoeae

Neisseria gonorrhoeae DNA Recombination and Repair Enzymes Protect against Oxidative Damage Caused by Hydrogen Peroxide

Contact Info

Product

Resources

About

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells