Inhibition of <i>Vorticetta microstoma</i> Stalk Formation by Wheat Germ Agglutinin

Bramucci, M G; Nagarajan, Vasantha

doi:10.1111/j.1550-7408.2004.tb00389.x

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…Although swimming Vorticella cells could be utilized similarly to C. reinhardtii and P. caudatum , the swimming of stalkless Vorticella has been rarely studied. If swimming Vorticellae are to be used, furthermore, their stalk formation will need to be inhibited [ 160 ].…”

Section: Discussion and Future Outlookmentioning

confidence: 99%

Vorticella: A Protozoan for Bio-Inspired Engineering

Ryu¹,

Pepper

Nagai

2016

Micromachines

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In this review, we introduce Vorticella as a model biological micromachine for microscale engineering systems. Vorticella has two motile organelles: the oral cilia of the zooid and the contractile spasmoneme in the stalk. The oral cilia beat periodically, generating a water flow that translates food particles toward the animal at speeds in the order of 0.1–1 mm/s. The ciliary flow of Vorticella has been characterized by experimental measurement and theoretical modeling, and tested for flow control and mixing in microfluidic systems. The spasmoneme contracts in a few milliseconds, coiling the stalk and moving the zooid at 15–90 mm/s. Because the spasmoneme generates tension in the order of 10–100 nN, powered by calcium ion binding, it serves as a model system for biomimetic actuators in microscale engineering systems. The spasmonemal contraction of Vorticella has been characterized by experimental measurement of its dynamics and energetics, and both live and extracted Vorticellae have been tested for moving microscale objects. We describe past work to elucidate the contraction mechanism of the spasmoneme, recognizing that past and continuing efforts will increase the possibilities of using the spasmoneme as a microscale actuator as well as leading towards bioinspired actuators mimicking the spasmoneme.

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Section: Discussion and Future Outlookmentioning

confidence: 99%

Vorticella: A Protozoan for Bio-Inspired Engineering

Ryu¹,

Pepper

Nagai

2016

Micromachines

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“…V. microstoma is a very resistant protist recognised for taking up heavy metals such as lead, cadmium, copper, nickel and mercury ions [226,227]. It also forms stalks after their free-swimming stage, however, Bramucci and Nagarajan [228] showed that in presence of inhibitors they could stay in such stage. Interestingly, activated sludge was more favourable for V. microstoma at both WWTPs (3.2% in Garmerwolde and 1.2% in Vroomshoop) than granular sludge (< 1% in all the samples).…”

Section: Discussionmentioning

confidence: 99%

Pathogen removal in aerobic granular sludge treatment systems

Hernández¹

2022

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Recognition must be given to all the laboratory staff at IHE Delft, Fred, Berend, Peter, Lyzette, Ferdi, Frank, Zina. Your always positive collaboration made my going (up and ii downs stairs) easier. Thank you for your trust, support and provisions in this project. Berend Lolkema, without your friendship, prompt solutions, imagination, creativity and your always determination to help, we (my students and I) could not have gone through all our professional ups and downs. A genuine thank you for all our chats. Peter Heerings, E. coli and I could not survive without you at IHE lab. Thank you for your support in the microbiology lab and for your parental caring.I also would like to thank my colleagues from TU Delft, Mona, Bruno for the tailormade training and determination to share your experience with me. A unique appreciation is given to Dr. Danny de Graaff. Your coaching skills in the first year of my PhD were noteworthy in this research project.Carolien Boersma, "A picture is worth a thousand words." (Brisbane, A). Thank you for making that possible in this book.The list of friends and acquaintances on the road is extensive and may be incomplete. There have been many years of seeing people coming and going as passengers, but some stayed on the trip a bit longer. Iosif, my friend, you got off the bus at an earlier station, but thanks to your friendship, I did not feel alone at IHE during lunchtimes; you are a great reliable person. Yuli, I found a friend when I met you. Thank you for the Indonesian lunches we shared and the times we laughed together. Dr. Ahmed, my officemate and adopted brother, thank you for your professional and wholehearted support. Your commitment to research has always been my driver. Mohanad A., I am proud of you; your patience and perseverance are unique. Thank you for the endless chats and good ideas. Ex-office neighbours, Neyler, Alida thanks for the funny talks, advice and friendship. Mohaned Sousi, Muthasen, Taha, Angelica, Mauricio, Juanca, you were part of the journey at different time's life. It was pleasant sharing experiences and social gatherings with some of you guys.To my most authentic friends, Cris, Nela, Clari, an immense thanks for believing in me, your companion, visits the Netherlands and unconditional support were significant factors to accomplish this journey. You are sisters for me! Finally, and most importantly, my sincere appreciations to my family. Arne, thanks for becoming such an exceptional person in my life and being unconditionally supportive during the last years of my journey. Santiago, Federico, my lovely sons. You always made me forget the struggles of a PhD life; with you guys, this journey was an adventure. Thanks for keeping me motivated while doing this and for being such supportive boys. Mami, Papi, Tita, Karol, Leroy, Cali, Sahian, Berny, y todo el resto de la gigantesca familia, muchas gracias por estar ahí siempre, sin importar las condiciones. Su amor a distancia, video llamadas a mitad de la noche y fiestas en mi nombre me hicieron siempre sentir c...

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“…Typically it takes several hours. The chemical composition of the adhesive pad is not yet fully known [4], and a non-invasive method for detaching Vorticella has not been established, although mechanical detachment methods have been demonstrated [5,6]. One such method involves shaking a flask containing Vorticella, which loosens the attached cells and prevents them from adhering to the surface [5].…”

Section: Introductionmentioning

confidence: 99%

Non-Invasive Detachment of <i>Vorticella</i> from Calcium Alginate Membrane

Nagai

Asai

Fujita

2013

e-J. Surf. Sci. Nanotechnol.

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Non-invasive detachment of Vorticella from a substrate is essential for fabricating a microsystem based on Vorticella. Here, we dry sodium alginate solution and obtain sodium alginate films with fewer uncrosslinked sol regions. Calcium ions cross-link the alginate polymers of the membrane and form a calcium alginate gel. We first culture V. convallaria on the gel membrane. By dissolving the membrane with a chelating agent, we detatch cells non-invasively from the substrate and obtain cells with intact stalks, which can then be placed in a microsystem without waiting for the stalks to grow. The cells can be patterned by lift-off of the calcium alginate membrane. The proposed method provides new processing capabilities and allows for the design and fabrication of a system based on an actuator of Vorticella.

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Inhibition of Vorticetta microstoma Stalk Formation by Wheat Germ Agglutinin

Cited by 4 publications

References 16 publications

Vorticella: A Protozoan for Bio-Inspired Engineering

Vorticella: A Protozoan for Bio-Inspired Engineering

Pathogen removal in aerobic granular sludge treatment systems

Non-Invasive Detachment of <i>Vorticella</i> from Calcium Alginate Membrane

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