Inhibition of interaction between Staphylococcus aureus α-hemolysin and erythrocytes membrane by hydrolysable tannins: structure-related activity study

Abstract

The objective of the study was a comparative analysis of the antihemolytic activity against two Staphylococcus aureus strains (8325-4 and NCTC 5655) as well as α-hemolysin and of the membrane modifying action of four hydrolysable tannins with different molecular mass and flexibility: 3,6-bis-O-di-O-galloyl-1,2,4-triO -galloyl-β-d-glucose (T1), 1,2,3,4,5-penta-O-galloyl-β-d-glucose (T2), 3-O-galloyl-1,2-valoneoyl-β-d-glucose (T3) and 1,2-di-O-galloyl-4,6-valoneoyl-β-d-glucose (T4). We showed that all the compounds studied manifested antihemolytic effects in the range of 5-50 µM concentrations. However, the degree of the reduction of hemolysis by the investigated tannins was not uniform. A valoneoyl group-containing compounds (T3 and T4) were less active. Inhibition of the hemolysis induced by α-hemolysin was also noticed on preincubated with the tannins and subsequently washed erythrocytes. In this case the efficiency again depended on the tannin structure and could be represented by the following order: T1 > T2 > T4 > T3. We also found a relationship between the degree of antihemolytic activity of the tannins studied and their capacity to increase the ordering parameter of the erythrocyte membrane outer layer and to change zeta potential. Overall, our study showed a potential of the T1 and T2 tannins as anti-virulence agents. The results of this study using tannins with different combinations of molecular mass and flexibility shed additional light on the role of tannin structure in activity manifestation. Over the past few years, a significant increase in bacterial resistance to antibiotics and transference of resistance genes from animal to human strains has become a global medical problem. Therefore, constant search for new antimicrobial agents among them being compounds of plant origin, including polyphenols, is ongoing 1,2. In addition to the antibiotic approaches of combating bacteria, anti-virulence strategies have also been considered recently. An anti-virulent strategy assumes a direct effect of compounds on virulent factors by reducing

Effects of a Berry Polyphenolic Fraction on the Pathogenic Properties of Porphyromonas gingivalis

“…Interestingly, the berry polyphenolic fraction completely inhibited the hemolytic activity of P. gingivalis and as such, may impede its ability to proliferate. Although the mechanism involved in the inhibition of the P. gingivalis hemolytic activity by the berry polyphenolic fraction has not been investigated, it may be related to structural changes in erythrocyte membranes caused by the polyphenols thus limiting the binding of the hemolysin, as previously reported in Staphylococcus aureus [ 32 ].…”

Enzymatic synthesis and characterization of aryl iodides of some phenolic acids with enhanced antibacterial properties

“…We measured the fluorescence anisotropy values for TMA-DPH probe locating in the outer area of the membrane at the level of the fourth carbon atom and for DPH probe inserting itself in the membrane hydrophobic region in the middle of the lipid bilayer of red blood cells. The fluorescence anisotropy values were used to calculate the lipid order parameter [14,16,17] (S) and were presented as a relative change (S/S 0 ) in the presence of compounds compared to control (Figure 4C, D). These results reveal increased surface lipid ordering based on higher TMA-DPH S/S 0 values and lower lipid ordering in the middle of bilayer due to lower DPH S/S 0 values in the presence of both molecules.…”

Identification and Characterization of Glucosyltransferase That Forms 1-Galloyl-β-d-Glucogallin in Canarium album L., a Functional Fruit Rich in Hydrolysable Tannins

“…Strong antioxidant and radical scavenging capacities make HTs play a role in the treatment of various diseases. The antioxidant activity and scavengers of hydroxyl, superoxide, and peroxyl radicals largely depend on their structure [ 2 , 3 , 4 ]; for example, an increase in anti-radical effects was observed with an increase in the degree of polymerization [ 5 ]. Modern medical research has shown that HTs have pharmacological activities against COVID-19 [ 6 ], bacteria [ 4 ], inflammation [ 7 ], nephropathy [ 8 ], diabetes [ 9 ], HIV, and many other pathologies [ 5 , 10 , 11 ].…”