Inhibition of Intercellular Adhesion Molecule-1 Protein Expression by Antisense Oligonucleotides Is Neuroprotective After Transient Middle Cerebral Artery Occlusion in Rat

Vemuganti, Raghu; Dempsey, Robert J.; Bowen, Kellie K.

doi:10.1161/01.str.0000106479.53235.3e

Cited by 115 publications

(118 citation statements)

References 39 publications

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“…Total RNA (1 mg) from each sample was reverse transcribed with oligo(dT) 15 and random hexamer primers using M-MuLV reverse transcriptase (Life Technologies, Rockville, MD, USA). Real-time PCR was conducted as described earlier (Vemuganti et al, 2004). Ten nanograms of cDNA and MCP-1 primers (5 0 -3 0 , CCA GAA ACC AGC CAA CTC TCA and GGC AGC AAC TGT GAA CAA CAG) were added to SYBR Green PCR Master Mix (SYBR Green I Dye, AmpliTaq s DNA polymerase, dNTPs with dUTP and optimal buffer components; Applied Biosystems, Foster City, CA, USA) and subjected to PCR amplification (one cycle at 501C for 2 min, one cycle at 951C for 10 min, and 40 cycles at 951C for 15 s and 601C for 1 min) in a TaqMan 5700 Sequence Detection System (Applied Biosystems).…”

Section: Real-time Pcrmentioning

confidence: 99%

“…MCP-1 (500 ng/ml) or aCSF with 0.1% BSA was infused into the striatum (coordinates: anteriorposterior À0.3 mm, lateral 2.5 mm, dorsal-ventral 4 mm) of 6 normal rats using Alzet osmotic minipumps (Model 2001, DURECT Co.) with a flow rate of 1 mL/h for 7 days as described by our previous studies (Dempsey et al, 2003;Vemuganti et al, 2004). Rats were killed at day 7 of infusion.…”

Section: Mcp-1 Infusionmentioning

confidence: 99%

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Monocyte Chemoattractant Protein-1 Plays a Critical Role in Neuroblast Migration after Focal Cerebral Ischemia

Yan

Sailor

Lang

et al. 2007

J Cereb Blood Flow Metab

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244

210

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Transient focal ischemia is known to induce proliferation of neural progenitors in adult rodent brain. We presently report that doublecortin positive neuroblasts formed in the subventricular zone (SVZ) and the posterior peri-ventricle region migrate towards the cortical and striatal penumbra after transient middle cerebral artery occlusion (MCAO) in adult rodents. Cultured neural progenitor cells grafted into the non-infarcted area of the ipsilateral cortex migrated preferentially towards the infarct. As chemokines are known to induce cell migration, we investigated if monocyte chemoattractant protein-1 (MCP-1) has a role in post-ischemic neuroblast migration. Transient MCAO induced an increased expression of MCP-1 mRNA in the ipsilateral cortex and striatum. Immunostaining showed that the expression of MCP-1 was localized in the activated microglia and astrocytes present in the ischemic areas between days 1 and 3 of reperfusion. Furthermore, infusion of MCP-1 into the normal striatum induced neuroblast migration to the infusion site. The migrating neuroblasts expressed the MCP-1 receptor CCR2. In knockout mice that lacked either MCP-1 or its receptor CCR2, there was a significant decrease in the number of migrating neuroblasts from the ipsilateral SVZ to the ischemic striatum. These results show that MCP-1 is one of the factors that attract the migration of newly formed neuroblasts from neurogenic regions to the damaged regions of brain after focal ischemia.

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Section: Real-time Pcrmentioning

confidence: 99%

Section: Mcp-1 Infusionmentioning

confidence: 99%

Monocyte Chemoattractant Protein-1 Plays a Critical Role in Neuroblast Migration after Focal Cerebral Ischemia

Yan

Sailor

Lang

et al. 2007

J Cereb Blood Flow Metab

Self Cite

244

210

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“…Quantitative real-time PCR was conducted on the same RNA samples used for the GeneChip analysis as described previously (Vemuganti et al, 2004). Total RNA (2 mg) from each sample was reverse-transcribed with oligo(dT)15 and random hexamer primers using M-MuLV reverse transcriptase (Life Technologies).…”

Section: Quantitative Real-time Pcr Assaysmentioning

confidence: 99%

Endothelial cells promote neural stem cell proliferation and differentiation associated with VEGF activated Notch and Pten signaling

Sun

Zhou

et al. 2010

Developmental Dynamics

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To investigate whether and how endothelial cells affect neurogenesis, we established a system to co-culture endothelial cells and brain slices of neonatal rat and observed how subventricular zone cells differentiate in the presence of endothelial cells. In the presence of endothelial cells, neural stem cells increased in number, as did differentiated neurons and glia. The augmentation of neurogenesis was reversed by diminishing vascular endothelial growth factor (VEGF) expression in endothelial cells with RNA interference (RNAi). Microarray analysis indicated that expression levels of 112 genes were significantly altered by co-culture and that expression of 81 of the 112 genes recovered to normal levels following RNAi of VEGF in endothelial cells. Pathway mapping showed an enrichment of genes in the Notch and Pten pathways. These data indicate that endothelial cells promote neural stem cell proliferation and differentiation associated with VEGF, possibly by activating the Notch and Pten pathways. Developmental Dynamics 239:2345-2353,

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“…(Williams et al 2005). Tubulin is present in almost all eukaryotic cells and is commonly used as a housekeeping protein (Vemuganti et al 2004). …”

Section: Western Immunoblot Analysismentioning

confidence: 99%

The expression of transforming growth factor β in pregnant rat myometrium is hormone and stretch dependent

Shynlova

Tsui²,

Dorogin³

et al. 2007

Reproduction

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From a quiescent state in early pregnancy to a highly contractile state in labor, the myometrium displays tremendous growth and remodeling. We hypothesize that the transforming growth factor b (TGFb) system is involved in the differentiation of pregnant myometrium throughout gestation and labor. Furthermore, we propose that during pregnancy the mechanical and hormonal stimuli play a role in regulating myometrial TGFbs. The expression of TGFb1-3 mRNAs and proteins was examined by real-time PCR, Western immunoblot, and localized with immunohistochemistry in the rat uterus throughout pregnancy and labor. Tgfb1-3 genes were expressed differentially in pregnant myometrium. Tgfb2 gene was not affected by pregnancy, whereas the Tgfb1 gene showed a threefold increase during the second half of gestation. In contrast, we observed a dramatic bimodal change in Tgfb3 gene expression throughout pregnancy. Tgfb3 mRNA levels first transiently increased at mid-gestation (11-fold on day 14) and later at term (45-fold at labor, day 23). Protein expression levels paralleled the changes in mRNA. Treatment of pregnant rats with the progesterone (P4) receptor antagonist RU486 induced premature labor on day 19 and increased Tgfb3 mRNA, whereas artificial maintenance of elevated P4 levels at late gestation (days 20-23) caused a significant decrease in the expression of Tgfb3 gene. In addition, Tgfb3 was up-regulated specifically in the gravid horn of unilaterally pregnant rats subjected to a passive biological stretch imposed by the growing fetuses, but not in the empty horn. Collectively, these data indicate that the TGFb family contributes in the regulation of myometrial activation at term integrating mechanical and endocrine signals for successful labor contraction. Reproduction (2007) 134 503-511

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Inhibition of Intercellular Adhesion Molecule-1 Protein Expression by Antisense Oligonucleotides Is Neuroprotective After Transient Middle Cerebral Artery Occlusion in Rat

Cited by 115 publications

References 39 publications

Monocyte Chemoattractant Protein-1 Plays a Critical Role in Neuroblast Migration after Focal Cerebral Ischemia

Monocyte Chemoattractant Protein-1 Plays a Critical Role in Neuroblast Migration after Focal Cerebral Ischemia

Endothelial cells promote neural stem cell proliferation and differentiation associated with VEGF activated Notch and Pten signaling

The expression of transforming growth factor β in pregnant rat myometrium is hormone and stretch dependent

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