2001
DOI: 10.1074/jbc.m010260200
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Inhibition of Interleukin-4- and CD40-induced IgE Germline Gene Promoter Activity by 2′-Aminoethoxy-modified Triplex-forming Oligonucleotides

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Cited by 26 publications
(19 citation statements)
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“…Here we demonstrate the ability of antiparallel purine motif TFOs to selectively form DNA triplex at two sequences encoding tandem Ets core DNA binding motifs in the tie-1 promoter and the triplex-mediated inhibition of promoter activity through TFO binding to one of these sequences (E-1). Although the inhibition of the IgE germline proximal gene promoter through triplex DNA formation at a sequence regulated by STAT6, NF-κB, and the Ets factor PU.1 was reported recently (30), to our knowledge this is the first demonstration of selective triplex DNA formation at sequences containing multiple Ets binding sites.…”
Section: Discussionmentioning
confidence: 99%
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“…Here we demonstrate the ability of antiparallel purine motif TFOs to selectively form DNA triplex at two sequences encoding tandem Ets core DNA binding motifs in the tie-1 promoter and the triplex-mediated inhibition of promoter activity through TFO binding to one of these sequences (E-1). Although the inhibition of the IgE germline proximal gene promoter through triplex DNA formation at a sequence regulated by STAT6, NF-κB, and the Ets factor PU.1 was reported recently (30), to our knowledge this is the first demonstration of selective triplex DNA formation at sequences containing multiple Ets binding sites.…”
Section: Discussionmentioning
confidence: 99%
“…Triplex DNA formation has been investigated as a highly selective means of regulating many clinically relevant targets such as c-myc (25,31), cyclin D1 (24), mdr-1 (27), HIV (28), insulin-like growth factor-1 receptor (29), platelet-derived growth factor (32), rhodopsin (53), and the IgE germline gene promoter (30). In addition to the disruption of transcriptional elongation (21,22,(27)(28)(29)53), inhibition of gene expression has been achieved through triplex DNA formation at sequences encoding transcription factor binding sites including SP-1, SRF, CNBP, PuF, MAZ, Pax5, PU.1, STAT6, and NF-κB (21,22,(24)(25)(26)31,32).…”
Section: Discussionmentioning
confidence: 99%
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“…The labeled product was purified by size exclusion chromatography (G-25 Sephadex Microspin columns; Amersham Life Sciences) and polyacrylamide gel electrophoresis. The binding reaction with equal amounts of whole-cell extract was performed as described [45]. For supershift experiments, 1 lg of specific antibody was pre-incubated on ice for 30 min before addition of approximately 0.5 ng labeled ds-DNA and further incubation for 30 min on ice.…”
Section: Cells Cell Culture Antibodies Proliferationmentioning
confidence: 99%
“…A positive charge and an RNA-like sugar conformation have been joined in the 2′-O-(2-aminoethyl) (AE) ribose derivatives developed by Cuenoud and colleagues (55)(56)(57). TFOs carrying these substitutions show enhanced kinetics of triplex formation and greater stability of the resultant complex at physiological pH and low Mg ++ concentration.…”
Section: Oligonucleotide Modifications Improve Tfo Activity Under Phymentioning
confidence: 99%