Inhibition of Invasion and Metastasis During Specific Amino Acid Restriction Associated with Metastasis Suppressor and Other Gene Changes

Meadows, Gary G.; Ge, Xiaokang; Zhang, Hui; Fu, Yiming

doi:10.1007/0-306-47821-8_10

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…Our previous work showed that Vol. 45 the intracellular Tyr and Phe level in solid tumor cells like melanoma are significantly higher than those in HIFF cells and that deprivation of Tyr/Phe reduced their intracellular levels in melanoma cells but not in HIFF (59). This indicates that, for some specific amino acids, such as Tyr/Phe, normal cells require lower intracellular levels for their survival than do tumor cells.…”

mentioning

confidence: 85%

Specific Amino Acid Dependency Regulates Invasiveness and Viability of Androgen-Independent Prostate Cancer Cells

Fu¹,

Yu²,

Li³

et al. 2003

Nutrition and Cancer

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Androgen-independent prostate cancer is resistant to therapy and is often metastatic. Here we studied the effect of deprivation of tyrosine and phenylalanine (Tyr/Phe), glutamine (Gln), or methionine (Met), in vitro on human DU145 and PC3 androgen-independent prostate cancer cells, and on nontumorigenic human infant foreskin fibroblasts and human prostate epithelial cells. Deprivation of the amino acids similarly inhibited growth of DU145 and PC3 cells, arresting the cell cycle at G0/G1. Met and Tyr/Phe deprivation induces apoptosis in DU145, but only Met deprivation induces apoptosis in PC3 cells. The growth of normal cells is inhibited, but no apoptosis is induced by amino acid deprivation. Tyr/Phe deprivation inhibits expression and phosphorylation of focal adhesion kinase (FAK) and extracellular-regulated kinase (ERK) in DU145 but not PC3 or normal cells. Met deprivation inhibits phosphorylation but not protein expression of FAK and ERK in PC3. Therefore, apoptosis of DU145 and PC3 cells by amino acid restriction is FAK and ERK dependent. Tyr/Phe and Met deprivation inhibits invasion of DU145 and PC3, but Gln deprivation only inhibits invasion of DU145 cells. This indicates that the inhibition of invasion is not dependent on induction of apoptosis. The inhibition of invasion by Tyr/Phe restriction in DU145 and Met restriction in PC3 is consistent with the inhibition on FAK/ERK signaling. The inhibition of Tyr/Phe restriction in PC3 and Gln restriction in DU145 is not associated with inhibition of FAK/ERK. This indicates that FAK/ERK-dependent and independent pathways are modulated by specific amino acid restriction. This study shows the potential for specific amino acid restriction to treat prostate cancer.

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confidence: 85%

Specific Amino Acid Dependency Regulates Invasiveness and Viability of Androgen-Independent Prostate Cancer Cells

Fu¹,

Yu²,

Li³

et al. 2003

Nutrition and Cancer

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“…On the other hand, our laboratory, when looking at amino acids, showed that restriction of tyrosine and phenylalanine in vitro increases MKK4 protein expression by 2.5-fold in A375 melanoma cells [34] and also leads to increased p38 protein expression [27]. These changes were associated with an 80 % decrease in invasion [35]. Although the ability of tyrosine and phenylalanine restriction to inhibit metastasis of A375 melanoma or other human tumors has not been studied, dietary restriction of these amino acids inhibits metastasis in a wide variety of rodent tumors [36].…”

Section: Glucosinolatesmentioning

confidence: 90%

Diet, nutrients, phytochemicals, and cancer metastasis suppressor genes

Meadows

2012

Cancer Metastasis Rev

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The major factor in the morbidity and mortality of cancer patients is metastasis. There exists a relative lack of specific therapeutic approaches to control metastasis, and this is a fruitful area for investigation. A healthy diet and lifestyle not only can inhibit tumorigenesis but also can have a major impact on cancer progression and survival. Many chemicals found in edible plants are known to inhibit metastatic progression of cancer. While the mechanisms underlying antimetastatic activity of some phytochemicals are being delineated, the impact of diet, dietary components, and various phytochemicals on metastasis suppressor genes is underexplored. Epigenetic regulation of metastasis suppressor genes promises to be a potentially important mechanism by which dietary components can impact cancer metastasis since many dietary constituents are known to modulate gene expression. The review addresses this area of research as well as the current state of knowledge regarding the impact of diet, dietary components, and phytochemicals on metastasis suppressor genes.

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Activation of caspases and cleavage of Bid are required for tyrosine and phenylalanine deficiency-induced apoptosis of human A375 melanoma cells

et al. 2002

Archives of Biochemistry and Biophysics

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Inhibition of Invasion and Metastasis During Specific Amino Acid Restriction Associated with Metastasis Suppressor and Other Gene Changes

Cited by 4 publications

References 38 publications

Specific Amino Acid Dependency Regulates Invasiveness and Viability of Androgen-Independent Prostate Cancer Cells

Specific Amino Acid Dependency Regulates Invasiveness and Viability of Androgen-Independent Prostate Cancer Cells

Diet, nutrients, phytochemicals, and cancer metastasis suppressor genes

Activation of caspases and cleavage of Bid are required for tyrosine and phenylalanine deficiency-induced apoptosis of human A375 melanoma cells

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