Inhibition of laccase activity from Trametes versicolor by heavy metals and organic compounds

Lorenzo, María del Carmen Ricoy; Moldes, Diego; Couto, Susana Rodrı́guez; Sanromán, M.A.

doi:10.1016/j.chemosphere.2004.12.051

Cited by 107 publications

(55 citation statements)

References 20 publications

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“…ions under SSF (Sharma et al 2015). In agreement with our results, Lorenzo et al (2005) also found that higher copper concentration (20 mM) inhibited the laccase yield from Trametes versicolor by 40%. Laccase by Ganoderma sp.…”

Section: Production Of Laccase Under Ssfsupporting

confidence: 82%

Optimization of laccase production and its application in delignification of biomass

Bagewadi

2017

Int J Recycl Org Waste Agricult

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Purpose Current research focuses on the biological delignification of biomass by microbial laccase which is an environmentally friendly process. Methods Various statistical approaches were designed for optimization of laccase production like Plackett-Burman design as well as response surface methodology (RSM). A laccase mediator system was designed for the delignification of saw dust which was molecularly characterized by high-performance liquid chromatography (HPLC), Fourier transformation infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Results The present study reveals wheat bran is a potential substrate for the production of laccase (63 U/g and 9.6 mg/ g protein) under solid-state fermentation by Trichoderma harzianum strain HZN10. Statistical optimization by RSM using central composite design (CCD) revealed that wheat bran contributed maximally to the overall laccase production followed by yeast extract. Laccase production under optimized conditions yielded 510 U/g with 8.09-fold increase. HPLC peaks representing 4-hydroxy-3-methoxybenzoic (vanillic) acid and 4-hydroxy-3,5-dimethoxybenzoic (syringic) acid showed drastic reduction in laccasetreated saw dust sample indicating the elimination of toxic inhibitors, thereby signifying the detoxification of sample.The laccase-treated saw dust showed 1.6-fold increase in reducing sugars after enzymatic (cellulase) hydrolysis. The FTIR analysis revealed the structural alterations occurring during the delignification process. SEM of biologically treated saw dust revealed the morphological alterations during the delignification process targeting the fiber cell walls rich in lignin. Conclusion The delignification of saw dust was effective by laccase mediator system and was evidenced by HPLC, FTIR and SEM analysis. Hence, laccase can be a powerful tool in biomass to biofuel conversions.

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Section: Production Of Laccase Under Ssfsupporting

confidence: 82%

Optimization of laccase production and its application in delignification of biomass

Bagewadi

2017

Int J Recycl Org Waste Agricult

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“…In contrast, even high concentrations of EDTA did not inhibit ABTS oxidation by a laccase produced by Coriolus hirsutus (21). Recently, Lorenzo et al (9) found that the inhibitory effect of EDTA on laccase activity was dependent on the substrate used. These authors observed a significant reduction in laccase activity in the presence of EDTA when syringaldazine or dimethoxyphenol was used, whereas no inhibition of ABTS oxidation was detected under the same experimental conditions.…”

Section: Effect Of Inhibitory Substancesmentioning

confidence: 96%

Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus

Machado¹,

Matheus²

2006

Braz. J. Microbiol.

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Pleurotus ostreatus ("shimeji") is produced in Brazil on a commercial scale using various lignocellulosic residues. Efforts have been made to reuse the culture residue to obtain products of greater aggregate value such as enzymes or in processes of bioremediation. We evaluated the Remazol brilliant blue R (RBBR) degradation potential of extracts from solid substrate colonized by P. ostreatus and extracts from residue of the "shimeji" mushroom yield. Colonized substrates and residue were provided by Toyobo do Brasil Ltda. Extraction was performed with sodium acetate buffer (50 mM, pH 4.6). RBBR decolorization was monitored at 592 nm and peroxidase and laccase activities were measured by monitoring the oxidation of ABTS. Horseradish peroxidase was used as reference. The time of growth of P. ostreatus influenced RBBR degradation and peroxidase and laccase activities. Concentration of 1 mM H2O2 and pH 4.0 were the best for RBBR decolorization. Complete RBBR decolorization was obtained with the addition of only one aliquot of 50 µL of 1 mM H2O2. The stability of the extracts was higher when they were kept under refrigeration than when stored frozen. The potential application of the ligninolytic complex derived from P. ostreatus and mushroom residue for xenobiotic degradation was demonstrated.

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“…Murugesan et al (2009) reported that Ca 2+ , Co 2+ , Cu 2+ , and Zn 2+ enhanced the laccase activity of Ganoderma lucidum up to a 1.0 mM concentration and that chemical dyes were decolorized during 1 h of incubation in the presence of 1.0 mM Cu 2+ . Lorenzo et al (2005) reported that the addition of Cu 2+ to the reaction mixture stimulated laccase activity at concentrations lower than 1.0 mM and that Mn 2+ and Zn 2+ did not affect enzyme activity at any of the concentrations tested (0.5 mM to 80 mM). The filling of the Type I Copper binding site by Cu 2+ could be the main cause of this increased laccase activity (Shekher et al 2011;Sondhi et al 2014).…”

Section: Laccase Production and Its Effects On Biomass Degradationmentioning

confidence: 99%

Characterization of a Novel Laccase-producing Bacillus sp. A4 and its Application in Miscanthus Degradation

Guo¹,

Lin²,

Wang³

et al. 2017

BioResources

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Bacillus sp. A4 exhibiting laccase production was isolated from forest soil. Its laccase secreted into a LB medium exhibited a maximum activity of 3.9 U mg -1 protein at the optimal temperature (37 °C) and pH (6.0). The purified laccase of Bacillus sp. A4 demonstrated a low molecular mass of 33 kDa, and its optimal temperature and pH were 40 °C and 4.6, respectively, when using ABTS as a substrate. The activity of the purified laccase was significantly increased in the presence of Cu 2+ , methanol, and ethanol, but it was totally inhibited by L-cysteine. The laccase production of this strain was markedly stimulated when the strain was incubated with 0.5% different lignocellulosic biomasses. The highest activity of laccase (22.6 U mg -1 protein) was obtained in using algal biomass. This new strain efficiently decreased the lignin content of lignocellulose biomasses after 9 d of incubation at 37 °C, especially lignin from grasses. Further analysis showed that, compared to that of all tested biomasses, the new strain was a more efficient decomposer of the lignin of Miscanthus, which exhibited much more lignin loss and cell wall structure destruction in a short span of time. Therefore, the potential use of this strain could be advantageous for using lignin in Miscanthus for industrial processes.

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Inhibition of laccase activity from Trametes versicolor by heavy metals and organic compounds

Cited by 107 publications

References 20 publications

Optimization of laccase production and its application in delignification of biomass

Optimization of laccase production and its application in delignification of biomass

Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus

Characterization of a Novel Laccase-producing Bacillus sp. A4 and its Application in Miscanthus Degradation

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