Inhibition of Laser-Induced Choroidal Neovascularization by Hematoporphyrin Dimethylether-Mediated Photodynamic Therapy in Rats

Yan, Yuan; Bao, Leilei; Zhang, Lijun; Bian, Jun; Hu, Tai‐Shan; Min, Zheng; Chen, Dan-Ye; Yu, Xinhai; Chen, Zhilong

doi:10.1248/bpb.b17-00319

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…Laser irradiation destroys or induces necrosis in retinal cells including RPE cells [49] and therefore likely results in the exposure of remaining intact RPE cells to the contents of necrotic cells. Several cytokines such as IL-6, IL-8, and monocyte chemoattractant protein–1 have been implicated in CNV [50, 51], and we previously showed that NLR contains these cytokines [16].…”

Section: Resultsmentioning

confidence: 99%

Inhibitory effect of nintedanib on VEGF secretion in retinal pigment epithelial cells induced by exposure to a necrotic cell lysate

Hatano¹,

Tokuda²,

Kobayashi³

et al. 2019

PLoS ONE

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Necrosis is a form of cell death that results in rupture of the plasma membrane and the release of cellular contents, and it can give rise to sterile inflammation in the retina and other tissues. The secretion of vascular endothelial growth factor (VEGF) by retinal pigment epithelial (RPE) cells contributes to retinal homeostasis as well as to pathological angiogenesis. We have now examined the effect of a necrotic cell lysate prepared from human RPE cells (NLR) on the release of VEGF by healthy RPE cells. We found that NLR markedly increased the release of VEGF from RPE cells and that this effect was attenuated by nintedanib, a multiple receptor tyrosine kinase inhibitor, whereas it was unaffected by inhibitors of NF-κB signaling or of caspase-1. NLR also induced the phosphorylation of extracellular signal–regulated kinase (Erk) and signal transducer and activator of transcription 3 (Stat3) in a manner sensitive to inhibition by nintedanib, although inhibitors of Erk and Stat3 signaling pathways did not affect NLR-induced VEGF secretion. In addition, nintedanib attenuated the development of choroidal neovascularization in mice. Our results have thus shown that a necrotic lysate of RPE cells induced VEGF secretion from healthy RPE cells and that this effect was mediated by receptor tyrosine kinase signaling. They therefore suggest that VEGF secretion by healthy RPE cells is a potential therapeutic target for retinal diseases associated with sterile inflammation and pathological angiogenesis.

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Section: Resultsmentioning

confidence: 99%

Inhibitory effect of nintedanib on VEGF secretion in retinal pigment epithelial cells induced by exposure to a necrotic cell lysate

Hatano¹,

Tokuda²,

Kobayashi³

et al. 2019

PLoS ONE

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“…e oligomer consists of unsymmetrical metal-dielectric (Au/Si) nm, and this hybrid nanoparticle oligomer can be fine-tuned by fs laser-induced fusion of nanoscale gold nm to achieve Fano resistance [3]. Yan et al [4] research was designed to examine the impact of hematoporphyrin dimethyl ether (HDME)-mediated photodynamic treatment on laser-induced plexiform choroidal neovascularization (CNV) in mature Brown Norway mice [4]. Laser-induced fluorescence spectroscopy has the characteristics of high sensitivity, and fast and accurate monitoring.…”

Section: Related Workmentioning

confidence: 99%

Quantitative of Atomic Spectra by Laser-Induced Teaching Integrating Multitarget Tracking Algorithm

Zhang

Liu

Zhang

2022

Mobile Information Systems

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Laser-induced breakdown spectroscopy refers to the radiation spectrum formed by the vaporization and ionization of samples under high-temperature conditions. It is a new technology for detecting and analyzing the composition and content of materials. However, this technology is not yet very mature; in this regard, this paper proposes a quantitative study of atomic spectroscopy using laser-induced technology. On this basis, a method using laser-induced breakdown spectroscopy is proposed, and its quantitative analysis and application are carried out in detail. The laser-induced breakdown technology uses a high-power pulsed laser to form a plasma on the surface of the test piece and analyzes its radiation spectrum to obtain the composition and content of its elements. It also used the laser-induced breakdown technique to quantitatively analyze the Ti-doped Al2O3 ceramic system. Through the quantitative analysis of constant laser and constant plasma temperature, the linear correlation coefficient R2 of the calibration curve calculated with constant plasma temperature is obtained as 0.985, and the relative error is 5.3%. This paper uses a fixed laser pulse amplification voltage, the linear coefficient of the proportional relationship curve is only 0.92, and the error rate is 8.9%. By comparing the two curves, the Ti proportional curve obtained by a fixed plasma temperature is obtained in this paper. Its linearity is better than the Ti scaling curve obtained by constant laser voltage.

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Photosensitizer formulations in photodynamic therapy of age-related macular degeneration

Beirão,

Pereira,

Fernandes

et al. 2025

European Journal of Medicinal Chemistry

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Inhibition of Laser-Induced Choroidal Neovascularization by Hematoporphyrin Dimethylether-Mediated Photodynamic Therapy in Rats

Cited by 3 publications

References 42 publications

Inhibitory effect of nintedanib on VEGF secretion in retinal pigment epithelial cells induced by exposure to a necrotic cell lysate

Inhibitory effect of nintedanib on VEGF secretion in retinal pigment epithelial cells induced by exposure to a necrotic cell lysate

Quantitative of Atomic Spectra by Laser-Induced Teaching Integrating Multitarget Tracking Algorithm

Photosensitizer formulations in photodynamic therapy of age-related macular degeneration

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