Inhibition of limb chondrogenesis in vitro by vitamin A: Alterations in cell surface characteristics

Lewis, Cindy; Pratt, Robert M.; Pennypacker, John P.; Hassell, John R.

doi:10.1016/0012-1606(78)90058-1

Cited by 149 publications

(43 citation statements)

References 26 publications

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“…Epidermal cells, for example, adhere preferentially to type IV collagen by a process that is not stimulated by fibronectin (16). Many hours are required for epidermal cells to attach, suggesting that they synthesize their own attachment factor, although this has not yet been shown.Although fibronectin is produced by limb mesenchymal cells (7, 17), it is not produced by differentiated chondrocytes in cartilage (7,17,18 (20), type III from fetal calf skin (21), and type IV from a murine sarcoma (22).Chromatographic Procedures. Affinity chromatography.…”

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confidence: 99%

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Identification of an adhesion factor for chondrocytes.

Hewitt¹,

Kleinman²,

Pennypacker³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

108

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The attachment of chondrocytes to collagen substrates is stimulated by serum but not by fibronectin. The active material in serum was partially purified and was shown to be a protein by its sensitivity to trypsin and heat and its chromatographic properties. This factor, which we have named chondronectin, is distinct from fibronectin and does not stimulate fibroblast attachment. Because material with similar attachment-enhancing activity is produced by chondrocytes and is extractable from cartilage, c ondronectin may be a chondrocyte-specific attachment protein.Collagen substrates promote the attachment (1), growth (2), and differentiation (3, 4) of celb in culture and presumably in vivo. It is now known that fibroblasts and some other cell types do not bind directly to collagen (1, 5). Rather, fibronectin, a large glycoprotein produced by fibroblasts (6) and many other cell types (7-12), binds these cells to collagen (13). Fibronectin is also present in serum (12) at a high concentration (:t200-300 Mg/ml) and attaches freshly trypsinized cells to their substrates.Fibronectin-free serum as well as pure fibronectin can be prepared by affinity chromatography on immobilized collagen (14, 15) and used to test the requirements of various cells for attachment to collagen.Fibronectin, however, does not mediate attachment of all cell types to collagen. Epidermal cells, for example, adhere preferentially to type IV collagen by a process that is not stimulated by fibronectin (16). Many hours are required for epidermal cells to attach, suggesting that they synthesize their own attachment factor, although this has not yet been shown.Although fibronectin is produced by limb mesenchymal cells (7, 17), it is not produced by differentiated chondrocytes in cartilage (7,17,18 (20), type III from fetal calf skin (21), and type IV from a murine sarcoma (22).Chromatographic Procedures. Affinity chromatography. Fibronectin was removed from serum by adsorption to a collagen-Sepharose 4B column (14, 15). In these experiments, the adsorbant was first equilibrated with F12 medium and then incubated with fetal calf serum (GIBCO) for 1 hr at room temperature. Unbound material was washed from the column with F12 medium, and bound fibronectin was eluted with 1 M KBr in 0.05 M Tris-HCI, pH 5.3, containing 0.025 M 6-aminohexanoic acid. The isolated fibronectin fraction was dialyzed against fresh F12 before use. DEAE-cellulose column chromatography. Chondrocyte attachment activity was precipitated from serum with ammonium sulfate (30% of saturation). The precipitated material was redissolved in 6 M urea, dialyzed against 0.05 M Tris*HCI, pH 7.4, and chromatographed at 4°C on a DEAE-cellulose column equilibrated with the same buffer. Bound material was eluted from the column with a linear gradient of 0 to 1 M NaCl. Fractions (5 ml) were collected and aliquots of each fraction were dialyzed against F12 and assayed for attachment-enhancing activity for chondrocytes and for CHO cells. RESULTSCharacteristics of Chondrocyte Attachment. The ro...

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“…Although fibronectin is produced by limb mesenchymal cells (7,17), it is not produced by differentiated chondrocytes in cartilage (7,17,18). Because (20), type III from fetal calf skin (21), and type IV from a murine sarcoma (22).…”

mentioning

confidence: 99%

Identification of an adhesion factor for chondrocytes.

Hewitt¹,

Kleinman²,

Pennypacker³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

108

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“…These facts support the hypothesis that cell contacts are necessary for the chondrogenesis of mesenchyme cells. Some recent reports also lend support : cartilage formation was promoted by cell aggregation in micro-mass cultures (2), cell contacts have been mediated by macromolecules (16) and cell contacts have been established in pellet cultures (12).…”

Section: Discussionmentioning

confidence: 86%

Effect of Cell Association on <I>in vitro</I> Chondrogenesis of Mesenchyme Cells from Quail Limb Buds

Matsutani

Kuroda

1980

Cell Struct. Funct.

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ABSTRACT. Mesenchyme cells dissociated from the limb buds of quail embryos (stages 21-22) were used to study the effects of different types of cell association for various periods on cartilage differentiation. The mesenchyme cells were first incubated for 4-48 h as monolayers, precipitated pellets , or cultures with gyratory shaking or reciprocal shaking. The cells then were dissociated, and their ability to form cartilage colonies in cultures at low cell density was examined. Cells that had been incubated as monolayers or in cultures with reciprocal shaking formed only fibroblastic colonies , whereas cells that had been incubated in cultures with gyratory shaking for 12 h or as pellets for 16 h formed cartilage colonies. With the last two treatments, the number of cartilage colonies increased according to the prior incubation period. Thus, three-dimensional cell association for a certain period may stabilize the ability of mesenchyme cells to differentiate into cartilage cells .

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“…It was well established that RA-mediated gene repression is both necessary and sufficient for chondroblast differentiation (42). atRA has been shown to inhibit chondrogenic differentiation of embryonic mesenchymal cells and to cause loss of differentiated chondrocyte phenotype (43)(44)(45), suggesting that similar effects on cartilage differentiation in vivo may be responsible for abnormalities in the developing palate (46 -48).…”

Section: Cloning and Identification Of The Novel Gene Mcpr1-duringmentioning

confidence: 99%

Identification and Characterization of a Novel Gene, Mcpr1, and Its Possible Function in the Proliferation of Embryonic Palatal Mesenchymal Cells

Dongying

Deng

et al. 2006

Journal of Biological Chemistry

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Inhibition of limb chondrogenesis in vitro by vitamin A: Alterations in cell surface characteristics

Cited by 149 publications

References 26 publications

Identification of an adhesion factor for chondrocytes.

Identification of an adhesion factor for chondrocytes.

Effect of Cell Association on <I>in vitro</I> Chondrogenesis of Mesenchyme Cells from Quail Limb Buds

Identification and Characterization of a Novel Gene, Mcpr1, and Its Possible Function in the Proliferation of Embryonic Palatal Mesenchymal Cells

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