Inhibition of lipase and inflammatory mediators by Chlorella lipid extracts for antiacne treatment

Sibi, G

doi:10.4103/2231-4040.150364

Cited by 28 publications

(13 citation statements)

References 38 publications

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“…Data showed that Chlorella promotes inhibitory effects over P. acnes through attenuation of lipase activity. Also, Chlorella extracts modulated oxidative and inflammatory responses from human peripheral blood mononuclear cells stimulated by heat-killed P. acnes [19]. Sun et al (2014) proposed the use of selenium-enriched C. vulgaris cultures as means to induce bioaccumulation, producing antioxidant-rich biomass with applications in agriculture and human diet.…”

Section: Dietary Use and Effects Of Chlorella On Healthmentioning

confidence: 99%

“…Also, analysis of fatty acid composition obtained from C. vulgaris indicates that, among the 19 different fatty acids found, 5 were saturated and 14 were C14 to C24 unsaturated fatty acids [19]. Bewicke and Potter (1993), in a book entitled "Chlorella: The Emerald Food", highlight the beneficial possibilities of including Chlorella in the western diets, these are: high growth rate, high protein content, resistance to climatic variations, high nutritive value and digestibility, palatability, economical production and others [20].…”

Section: Chlorella Is Rich In Polyunsaturated Fatty Acidsmentioning

confidence: 99%

“…In a recent in vitro study, Sibi (2015) evaluated the antimicrobial activity of lipidic extracts from Chlorella microalgae (including C. vulgaris) against Propionibacterium acnes strains. Data showed that Chlorella promotes inhibitory effects over P. acnes through attenuation of lipase activity.…”

Section: Dietary Use and Effects Of Chlorella On Healthmentioning

confidence: 99%

“…Human Sibi, 2015 To study the response of Chlorella against Propionibacterium acnes through microdilution and in vitro with human peripheral blood mononuclear cells.…”

Section: Obesity and Fatty Acidsmentioning

confidence: 99%

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Chlorella vulgaris as a Source of Essential Fatty Acids and Micronutrients: A Brief Commentary

Freitas¹

2017

TOPSJ

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Polyunsaturated fatty acids (PUFAs) comprise about 35-40% of the total lipid content from green algae Chlorella, reaching up to 24% linoleic acid and 27% α-linolenic acid in C. vulgaris. Also, microalgae nutrient composition may be modulated by changes in the culture medium, increasing fatty acid and microelement concentrations in the algae biomass. PUFAs, such as α-linolenic (n-3) and linoleic (n-6) acids, as well as its derivatives, are considered essential for dietary consumption, and their ability to regulate body chemistry has been recently explored in depth. A balanced fatty acid consumption is shown to counteract the negative effects of western diets, such as chronic inflammation and glucose intolerance. In this brief commentary, technological and practical uses of C. vulgaris are explored as means to improve dietary quality and, ultimately, human health.

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Section: Dietary Use and Effects Of Chlorella On Healthmentioning

confidence: 99%

Section: Chlorella Is Rich In Polyunsaturated Fatty Acidsmentioning

confidence: 99%

Section: Dietary Use and Effects Of Chlorella On Healthmentioning

confidence: 99%

“…Human Sibi, 2015 To study the response of Chlorella against Propionibacterium acnes through microdilution and in vitro with human peripheral blood mononuclear cells.…”

Section: Obesity and Fatty Acidsmentioning

confidence: 99%

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Chlorella vulgaris as a Source of Essential Fatty Acids and Micronutrients: A Brief Commentary

Freitas¹

2017

TOPSJ

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“…Several compounds or extracts inhibiting P. acnes lipase have been identified, including extracts of green algae ( Chlorella ) and of the plant Terminalia chebula , polyhydroxylated fullerene , and vitamins B 2 , B 5 , K 3 , and K 5 . However, the molecular mechanisms involved remain unclear.…”

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confidence: 99%

Inhibition of Propionibacterium acnes lipase activity by the antifungal agent ketoconazole

Unno

Cho

Sugita

2017

Microbiology and Immunology

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The common skin disease acne vulgaris is caused by Propionibacterium acnes. A lipase secreted by this microorganism metabolizes sebum and the resulting metabolites evoke inflammation in human skin. The antifungal drug ketoconazole inhibits P. acnes lipase activity. We previously showed that the drug also inhibits the growth of P. acnes. Thus, ketoconazole may serve as an alternative treatment for acne vulgaris, which is important because the number of antibioticresistant P. acnes strains has been increasing.Key words ketoconazole, lipase, Propionibacterium acnes.Acne vulgaris, one of the most common skin diseases worldwide, uniquely targets the sebaceous follicles of the face, chest, and back, affecting most adolescents (1). Several factors are involved in the development of acne vulgaris, including hormonal stimulation, increases in the levels of sebum, and bacterial colonization. Microbiologically, the Gram-positive anaerobic bacterium Propionibacterium acnes is responsible for the development of acne vulgaris when overgrowth occurs, although the microorganism is normally a commensal of sebaceous glands. One major virulence factor of the microorganism is a secretory lipase that acts on triglycerides to release free fatty acids (2). Palmitic acid stimulates the toll-like receptor 2-mediated inflammasome, which is associated with the release of interleukin-1, Th17 differentiation, and interleukin-17-mediated keratinocyte proliferation, while oleic acid stimulates adhesion, keratinocyte proliferation, and comedogenesis in P. acnes, also via the release of interleukin-1 (3, 4). Thus, lipase overexpression increases follicular development. Antibacterial agents including clindamycin, minocycline, and doxycycline are standard therapies for acne vulgaris (5). During the last decade, the number of antibiotic-resistant P. acnes strains has gradually increased (6, 7); therefore, a new approach to treatment that does not depend on antimicrobial agents is needed.We found that the antifungal drug ketoconazole potently inhibits P. acnes lipase activity. Five clinical isolates and the type strain (NBRC 107605) were examined. Lipase activity was measured using the 4-methyl umbelliferyl oleate (MUO) assay as described by Niki et al. (8) with some modifications. All strains were grown in brain heart infusion (BHI) broth, and 100 mL cell suspensions (5.0 Â 10 8 cells/mL) in BHI broth supplemented with ketoconazole (Wako Pure Chemical, Osaka, Japan) at final concentrations of 0, 0.125, 0.25 0.5,1, 2, 4, 8, 16, and 32 mg/mL were added to 96-well plates. The plates were incubated anaerobically at 37°C for 24 h. Supernatants (50 mL) were centrifuged and then mixed with 50 mL 0.4 mM MUO (SigmaAldrich, Tokyo, Japan) dissolved in 13 mM Tris-HCl, 0.15 M NaCl, and 1.3 mM CaCl 2 (pH 8.0). The mixtures were incubated for 30 min at 25°C. Enzymatic reactions were terminated by adding 100 mL 0.1 M sodium citrate (pH 4.2). The levels of 4-methylumbelliferone released by the lipase were measured using a fluorometric microplate reader (...

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Microalgae Potential Feedstock for the Production of Biohydrogen and Bioactive Compounds

Kadimpati

Sanneboina

Narasimha

et al. 2020

Clean Energy Production Technologies

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Inhibition of lipase and inflammatory mediators by Chlorella lipid extracts for antiacne treatment

Cited by 28 publications

References 38 publications

Chlorella vulgaris as a Source of Essential Fatty Acids and Micronutrients: A Brief Commentary

Chlorella vulgaris as a Source of Essential Fatty Acids and Micronutrients: A Brief Commentary

Inhibition of Propionibacterium acnes lipase activity by the antifungal agent ketoconazole

Microalgae Potential Feedstock for the Production of Biohydrogen and Bioactive Compounds

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