Inhibition of Lipid Peroxidation in Synaptosomes and Liposomes by Nitrates and Nitrites

Nicolescu, Adrian C.; Zavorin, S. I.; Turro, Nicholas J.; Reynolds, James N.; Thatcher, Gregory R. J.

doi:10.1021/tx025529j

Cited by 24 publications

(16 citation statements)

References 62 publications

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“…Like in our case, in the majority of experiments ELF-MF increases lipid peroxidation [16], [53], [60], [65]. We can propose that ELF-MF, through increasing the level of NO, is involved in the reduction of ILP in ischemic gerbils, because NO itself may directly inhibit lipid peroxidation by intercepting alkoxyl and peroxyl radical intermediates and thus terminating chain propagation reaction [66], [67]. Di Loreto et al [18] also proposed that ELF-MF can simultaneously activate pro- and antioxidants.…”

Section: Discussionsupporting

confidence: 69%

Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia

et al. 2014

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Magnetic field as ecological factor has influence on all living beings. The aim of this study was to determine if extremely low frequency magnetic field (ELF-MF, 50 Hz, 0.5 mT) affects oxidative stress in the brain of gerbils submitted to 10-min global cerebral ischemia. After occlusion of both carotid arteries, 3-month-old gerbils were continuously exposed to ELF-MF for 7 days. Nitric oxide and superoxide anion production, superoxide dismutase activity and index of lipid peroxidation were examined in the forebrain cortex, striatum and hippocampus on the 7th (immediate effect of ELF-MF) and 14th day after reperfusion (delayed effect of ELF-MF). Ischemia per se increased oxidative stress in the brain on the 7th and 14th day after reperfusion. ELF-MF also increased oxidative stress, but to a greater extent than ischemia, only immediately after cessation of exposure. Ischemic gerbils exposed to ELF-MF had increased oxidative stress parameters on the 7th day after reperfusion, but to a lesser extent than ischemic or ELF-MF-exposed animals. On the 14th day after reperfusion, oxidative stress parameters in the brain of these gerbils were mostly at the control levels. Applied ELF-MF decreases oxidative stress induced by global cerebral ischemia and thereby reduces possible negative consequences which free radical species could have in the brain. The results presented here indicate a beneficial effect of ELF-MF (50 Hz, 0.5 mT) in the model of global cerebral ischemia.

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Section: Discussionsupporting

confidence: 69%

Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia

et al. 2014

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“…Disulfides, notably diallyl disulfide (DADS), have been studied as garlic-derived chemopreventive agents that reduce ACF formation in the rat/azoxymethane model (23,24). Reports suggest that the disulfide group of DADS is responsible for the antiproliferative activity (16). DADS was reported to induce a G 2 -M phase cell cycle block in HCT-15 cells (25) and to be anti-antiproliferative in Caco-2 cells (24, 26).…”

Section: Discussionmentioning

confidence: 99%

Colon cancer chemoprevention by a novel NO chimera that shows anti-inflammatory and antiproliferative activity in vitro and in vivo

Hagos

Carroll²,

Kouznetsova³

et al. 2007

Molecular Cancer Therapeutics

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Chemopreventive agents in colorectal cancer possess either antiproliferative or anti-inflammatory actions. Nonsteroidal anti-inflammatory drugs (NSAID) and cyclooxygenase-2 inhibitors have shown promise, but are compromised by side effects. Nitric oxide donor NSAIDs are organic nitrates conjugated via a labile linker to an NSAID, originally designed for use in pain relief, that have shown efficacy in colorectal cancer chemoprevention. The NO chimera, GT-094, is a novel nitrate containing an NSAID and disulfide pharmacophores, a lead compound for the design of agents specifically for colorectal cancer. GT-094 is the first nitrate reported to reduce aberrant crypt foci (by 45%) when administered after carcinogen in the standard azoxymethane rat model of colorectal cancer. Analysis of proximal and distal colon tissue from 8-and 28-week rat/azoxymethane studies showed that GT-094 treatment reduced colon crypt proliferation by 30% to 69%, reduced inducible NO synthase (iNOS) levels by 33% to 67%, reduced poly(ADP-ribose)polymerase-1 expression and cleavage 2-to 4-fold, and elevated levels of p27 in the distal colon 3-fold. Studies in cancer cell cultures recapitulated actions of GT-094: antiproliferative activity and transient G 2 -M phase cell cycle block were measured in Caco-2 cells; apoptotic activity was examined but not observed; anti-inflammatory activity was seen in the inhibition of up-regulation of iNOS and endogenous NO production in lipopolysaccharide (LPS)-induced RAW 264.7 cells. In summary, antiproliferative, anti-inflammatory, and cytoprotective activity observed in vivo and in vitro support GT-094 as a lead compound for the design of NO chimeras for colorectal cancer chemoprevention. [Mol Cancer Ther 2007;6(8):2230 -9]

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“…Reaction of nitric oxide with superoxide causes formation of peroxynitrite that initiates lipid peroxidation via reaction of lipids with its decomposition products hydroxyl radical and nitrogen dioxide (Brookes et al, 1998;Rubbo et al, 1994). In contrast, nitric oxide itself may directly inhibit lipid peroxidation by intercepting alkoxyl and peroxyl radical intermediates thereby terminating chain propagation reactions (Nicolescu et al, 2002;Niziolek et al, 2003;Rubbo et al, 1994).…”

Section: Inhibition Of Lipid Peroxidationmentioning

confidence: 99%

Oxidants, antioxidants and the ischemic brain

Warner

Sheng

Batinić‐Haberle

2004

Journal of Experimental Biology

540

362

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SUMMARY Despite numerous defenses, the brain is vulnerable to oxidative stress resulting from ischemia/reperfusion. Excitotoxic stimulation of superoxide and nitric oxide production leads to formation of highly reactive products,including peroxynitrite and hydroxyl radical, which are capable of damaging lipids, proteins and DNA. Use of transgenic mutants and selective pharmacological antioxidants has greatly increased understanding of the complex interplay between substrate deprivation and ischemic outcome. Recent evidence that reactive oxygen/nitrogen species play a critical role in initiation of apoptosis, mitochondrial permeability transition and poly(ADP-ribose) polymerase activation provides additional mechanisms for oxidative damage and new targets for post-ischemic therapeutic intervention. Because oxidative stress involves multiple post-ischemic cascades leading to cell death, effective prevention/treatment of ischemic brain injury is likely to require intervention at multiple effect sites.

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Inhibition of Lipid Peroxidation in Synaptosomes and Liposomes by Nitrates and Nitrites

Cited by 24 publications

References 62 publications

Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia

Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia

Colon cancer chemoprevention by a novel NO chimera that shows anti-inflammatory and antiproliferative activity in vitro and in vivo

Oxidants, antioxidants and the ischemic brain

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