Inhibition of lipid peroxidation of herbal extracts (obtained from plant drug mixtures ofMyrtilli folium, Phaseoli fructus sine seminibusandSalviae folium) used in type 2 diabetes mellitus

Abstract

Plant drug mixtures are widely used in the adjuvant therapy of type 2 diabetes mellitus for the prevention of complications. The drug mixtures generally contribute to the efficiency of the therapy and may also reduce undesirable side effects. Two herbal extracts (lyophilized aqueous extracts of plant drug mixtures 1: Myrtilli folium, Phaseoli fructus sine seminibus and 2: Myrtilli folium, Phaseoli fructus sine seminibus, Salviae folium) were investigated in in vitro rat models. The content of bioactive constituents (polyphenol, flavonoid and vitamin C) in plant drug mixtures and lyophilized samples was evaluated. The antioxidant activity of lyophilized extracts was determined by measuring the ferric reducing ability of the plant, Fe2+ induced lipid peroxidation (LPO) in rat brain homogenates and NADPH (beta-nicotinamide adenine dinucleotide phosphate reduced form) induced LPO in cerebral microsomes. The antioxidant activity of lyophilized extracts was compared to that of quercetin and rutin. Both teas of lyophilized extracts had significant reducing ability (2694 and 2771 micromol/l) and inhibited LPO (IC50 28.0 and 20.6 microl in NADPH induced LPO, 17.3 and 8.7 microl in Fe2+ induced LPO). The high concentration of polyphenol/flavonoid (12.38-13.00 and 1.45-5.22 g/100 g, respectively) and vitamin C (0.099-0.165 g/100 g) in the herbal extracts is related to their significant antioxidant properties. The tea mixtures have significant nutritional value, since the consumption of 2 or 3 cups of tea a day covers 50% of the daily requirement of vitamin C and it is also relevant polyphenol source. The high polyphenol/flavonoid content may restore the redox imbalance and contribute to the prevention of diabetic complications.

An in vitro study of concentration specific antioxidant activity of commonly using herbs.

“…Diabetes mellitus (DM) has been known to be linked with increased oxidative injury (Yang et al, 2009;Szentmihalyi et al, 2010). Conversely, the excessively generated free radicals results numerous pathological complications including DM (Ganga et al, 2015).…”

“…As, routinely using herbs are rich source of polyphenols, flavonoids, saponins and other health boosting secondary metabolites, so specially in India they are used as home remedy as normal diet. Special reference to diseased person these are taken without prescription of physician (Szentmihalyi et al, 2010). Although, scientifically >800 herbs have been known to possess antioxidative as well as antidiabetic activities (Buyukbalci and Nehir, 2008) and are thought to devoid of side effects.…”

Antioxidant value and element content in some tinctures used in medication

“…Therefore active agents and elements in tincures affect relatively quickly in spite of their small amount [4]. Polyphenolic active agents, as flavonoids, tannins, have antioxidant activities [30] and their presence in the extracts, e.g. hesperidin in Tinctura Aurantii amari epicarpii et mesocarpii or tannins in Tinctura Ratanhiae, may be favourable in view of antioxidative value.…”

In vitro antioxidant activity of the individual herbs of DIA-2, a herbal mixture containing standardized extracts of Allium sativum and Lagerstroemia speciosa

“…In recent years, plants based medicines have been found to possess excellent antioxidant activities and used in combination to demonstrate significant therapeutic effects than when used alone (Yang et al, 2009). Plant drug combinations were widely used in the adjuvant therapy of type 2 diabetes mellitus for the prevention of complications (Szentmihalyi et al, 2010). DIA-2 is a polyherbal formulation formulated with an aim to combat hyperglycemia and hyperglycaemia induced oxidative stress.…”