Inhibition of lncRNA SNHG8 plays a protective role in hypoxia-ischemia-reoxygenation-induced myocardial injury by regulating miR-335 and RASA1 expression

Abstract

long non-coding (lnc)rnas serve a role in a number of diseases, including different types of cancer and acute myocardial infarction. The aim of the present study was to investigate the protective role of lncrna small nucleolar rna host gene 8 (SNHG8) in hypoxia-ischemia-reoxygenation (Hi/r)-induced myocardial injury and its potential mechanism of action. cell viability, proliferation, creatine kinase myocardial band, cell apoptosis and protein expression levels were determined by cell counting Kit-8 assay, edu assay, ELISA, flow cytometry and western blotting, respectively. The association between SNHG8 and microrna (mir)-335 was confirmed using a dual-luciferase reporter gene assay. The effects of the mir-335 inhibitor transfections had on increasing apoptosis and decreasing H9c2 cell viability were reversed in cells co-transfected with SNHG8 small interfering (si)rna. Furthermore, it was found that mir-335 could regulate raS p21 protein activator 1 (raSa1) expression and that transfection with SNHG8 sirna downregulated raSa1 expression. Silencing of raSa1 protected against Hi/r-induced H9c2 cell injury. However, SNHG8 sirna did not further reduce apoptosis, demonstrating that SNHG8 may act through raSa1, and raSa1 may mediate the protection of SNHG8 sirna in Hi/r myocardial injury. Thus, inhibition of lncrna SNHG8 alleviated Hi/r-induced myocardial damage by regulating mir-335 and raSa1.

Identification of Clinical Prognostic Regulators and Analysis of Ferroptosis-Related Signatures in the Tumor Immune Microenvironment in Lung Squamous Cell Carcinoma

“…The lncRNA HLA complex group 11 has been shown to diminish the malignancy of non-small-cell lung cancer by reducing the expression levels of carcinogenic microRNA875 [ 13 ]. However, transfection with lncRNA short nucleolar RNA host gene eight reduced RASA1 expressions, shielding H9C2 cells against HI/R damage [ 14 ]. Nonetheless, the association of lncRNAs with immune cell infiltration in ironophilic cell disease is yet unclear.…”

Targeting Epigenetics and Non-coding RNAs in Myocardial Infarction: From Mechanisms to Therapeutics

“…Presently, experiments concerning the functional role of lncRNAs were performed under experimental animal models or by in vitro assays. Some studies revealed the active role of lncRNAs in cell autophagy (Liang et al, 2020;Zhang and He, 2020;Li J. et al, 2021), apoptosis (Zhu et al, 2018;Gong et al, 2019;Zhang D. et al, 2019, Zhang M. et al, 2019Zhou et al, 2019;Huang L. et al, 2020;Liao et al, 2020;Lv et al, 2020;Yan et al, 2020;Zhang Y. et al, 2020;Zhou et al, 2020;Liu et al, 2021;Zhu et al, 2021a, 1), cardiac fibrosis (Wang X. et al, 2018, 30;Huang et al, 2019;Sun F. et al, 2019;Zhang JC. et al, 2019, 21;Lang et al, 2021;Zhang H. et al, 2021, 155-5), cardiac remodelling Zhang B.f. et al, 2020), inflammation, and angiogenesis (Chen ZL.…”

Potential biomarkers for predicting the overall survival outcome of kidney renal papillary cell carcinoma: an analysis of ferroptosis-related LNCRNAs

“…In non-small cell lung cancer, the LNCRNA HLA complex group 11 was demonstrated in reducing malignancy by eliminating the expression levels of carcinogenic microRNA875 [ 14 ]. However, transfection with LNCRNA short nucleolar RNA host gene eight reduced RASA1 expressions, shielding H9C2 cells against HI/R damage [ 15 ]. In recent years, ferroptosis of tumor cells has attracted a lot of attention as a new type of cell death that enables tumor cells in overcoming treatment resistance [ 16 , 17 ].…”