Inhibition of mammalian aspartate transcarbamylase by quinazolinone derivatives

Abstract

Quinazolinone derivatives have been studied as both in vitro and in vivo inhibitors of aspartate transcarbamylase (ATCase). In vitro treatment of mammalian ATCase with four compounds revealed that they inhibited enzyme activity and that 2-phenyl-1,3-4(H)benzothiazin-4-thione was the most potent one. This compound acts as a noncompetitive inhibitor towards both aspartate and carbamoyl phosphate. The values of the inhibition constant (K i) indicate that this compound exerts a potent inhibitory effect upon ATCase activity. Moreover, in vivo treatment with different doses of these derivatives showed also an inhibitory effect upon ATCase, the relative activity being decreased by 40%-58% with a 1 mg dose. These data support the inhibition of ATCase by quinazolinone derivatives as a new type of inhibitor for the enzyme.

Role of pH in Regulating Cancer Pyrimidine Synthesis

“…PALA may have antitumor activity and potentiates the effects of chemotherapeutic agents, e.g., 5-fluorouracil (5-FU) [ 41 ]. Moreover, 2-phenyl-1,3-4(H)benzothiazin-4-thione (quinazolinone derivative) is a strong ATCase inhibitor [ 42 ]. The pyrimidine pathway might form cytidine triphosphate (CTP); this CTP blocks ATCase, reflecting its negative feedback mechanism.…”