Inhibition of meiotic divisions of rat spermatocytes in vitro by polycyclic aromatic hydrocarbons

Georgellis, Antonis; Toppari, Jorma; Veromaa, Timo; Rydström, Jan; Parvinen, Martti

doi:10.1016/0027-5107(90)90019-z

Cited by 41 publications

(28 citation statements)

References 47 publications

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“…However, animal studies have detected PAH-DNA adducts in testicular tissues simultaneously revealing the possibility that PAHs can compromise the function of the barrier and affect spermatogenesis [6]. Additionally, the studies have shown PAH exposure could form PAH-DNA adducts in human sperm [4]. Prior to this study, limited available data existed about whether environmental and/or occupational exposure to PAHs contributes to the increased levels of bulky DNA adducts in human sperm.…”

Section: Introductionmentioning

confidence: 98%

“…Cellular deoxyribonucleic acid (DNA) has been suggested as a target in the subjects exposed to PAHs. Polycyclic aromatic hydrocarbon active intermediates, the byproducts of PAHs via cytochrome P450 (CYP450) phase I metabolism, have been reported to covalently attach to DNA in tissues and lymphocytes [4]. Also, active intermediates could take the redox cycling pathway and generate excess reactive oxygen species.…”

Section: Introductionmentioning

confidence: 99%

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Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons

Jeng

Pan

Chao

et al. 2016

Int J Occup Med Environ Health

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International Journal of Occupational Medicine and EnvironmentalThe objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. Material and Methods:The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. Results: The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. Conclusions: Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915-926

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons

Jeng

Pan

Chao

et al. 2016

Int J Occup Med Environ Health

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“…A body of evidence 4,[47][48][49] suggests that toxic exposures in premeiotic stages of male germ cell development may be responsible for adverse phenotypes in latter stages of spermatogenesis effecting mature sperm's motility, viability, impaired acrosome reaction, and subsequent fertilization suggesting that early exposures lead to late effects. 48,49 Previously, we have used an in vitro spermatocyte cell line to model how environmental toxins potentiate increases in oxidative stress, 5,50 providing further explanation for the mechanism behind abnormal semen parameters and perhaps subfertility seen in male smokers.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3][4][5] Although it is clear that one such PAH, 2,3,7,8 tetrachlorodibenzo-p-dioxin (ie, dioxins), acts through the aryl hydrocarbon receptor (AHR) to mediate toxicity, the specific mechanisms by which PAHs exert their toxic effect remain uncertain. 3,[6][7][8] Cigarette smoke, composed of over 4000 chemicals including known carcinogens such as PAHs, 9 has been linked to aberrant spermatogenesis and semen parameters associated with male factor infertility.…”

Section: Introductionmentioning

confidence: 99%

Modeling the Effect of Cigarette Smoke on Hexose Utilization in Spermatocytes

et al. 2015

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We set out to determine whether the addition of an aryl hydrocarbon receptor (AHR) antagonist has an effect on glucose/ fructose utilization in the spermatocyte when exposed to cigarette smoke condensate (CSC). We exposed male germ cells to 5 and 40 mg/mL of CSC + 10 mmol/L of AHR antagonist at various time points. Immunoblot expression of specific glucose/ fructose transporters was compared to control. Radiolabeled uptake of 2-deoxyglucose (2-DG) and fructose was also performed. Spermatocytes utilized fructose nearly 50-fold more than 2-DG. Uptake of 2-DG decreased after CSC þ AHR antagonist exposure. Glucose transporters (GLUTs) 9a and 12 declined after CSC þ AHR antagonist exposure. Synergy between CSC and the AHR antagonist in spermatocytes may disrupt the metabolic profile in vitro. Toxic exposures alter energy homeostasis in early stages of male germ cell development, which could contribute to later effects explaining decreases in sperm motility in smokers.

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“…Additionally, high concentrations of BaP and DMBA inhibited progression of spermatocytes through meiotic division (Georgellis et al , 1990 ;Schrader and Kanitz, 1994 ).…”

Section: Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%