Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract

Huang, Huey-Chun; Hsieh, Wan-Yu; Niu, Yulin; Chang, Tsong-Min

doi:10.1186/1472-6882-12-72

Cited by 45 publications

(33 citation statements)

References 33 publications

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“…Recently, antioxidants have been applied in skin care cosmetics for the prevention of hyperpigmentation. 8 oxygen species, resulting in cellular protection against UVA-irradiation. [9][10][11] However, whether silica hydride can affect melanogenesis or not has not been tested.…”

Section: Introductionmentioning

confidence: 99%

Inhibitions by Hydrogen-Occluding Silica Microcluster to Melanogenesis in Human Pigment Cells and Tyrosinase Reaction

Kato

Saitoh²,

Miwa³

2013

j. nanosci. nanotech.

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We investigated the anti-melanogenetic efficacy of hydrogen-occluding silica microcluster (H2-Silica), which is a silsesquioxane-based compound with hydrogen interstitially embedded in a matrix of caged silica, against melanogenesis in HMV-II human melanoma cells and L-DOPA-tyrosinase reaction [EC1.14.18.1]. HMV-II cells were subjected to oxidative stress by ultraviolet ray-A (UVA) exposure of 3-times of 0.65 J/cm2 summed up to 1.95 J/cm2. After UVA irradiation, HMV-II cells were stimulated to produce melanin by 2.72-fold more abundantly than unirradiated control. When HMV-II cells were treated with H2-Silica of 20 ppm or kojic acid of 28.4 ppm before and after UVA-irradiation, the amount of melanin was repressed to 12.2% or 14.5% as compared to that of UVA-irradiated control, respectively. That is, H2-Silica exhibited a comparable efficacy to the whitening agent kojic acid. The H2-Silica could prevent melanogenesis in HMV-II cells by low-level doses at 1-10 ppm, and cell viability and apoptosis event did not change even by high-level doses at 100-1000 ppm. On the contrary, kojic acid was cytotoxic at the concentration of 14-28 ppm or more. By microscopic observation, H2-Silica suppressed such properties indicative of melanin-rich cells as cellular hypertrophy, cell process formation, and melanogenesis around the outside of nuclei. The enzymatic assay using L-DOPA and mushroom tyrosinase demonstrated that H2-Silica restrained UVA-mediated melanin formation owing to down-regulation of tyrosinase activity, which could be attributed to scavenging of free radicals and inhibition of L-DOPA-to-dopachrome oxidation by hydrogen released from H2-Silica. Thus H2-Silica has a potential to prevent melanin production against UVA and serves as a skin-lightening ingredient for supplements or cosmetics.

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Section: Introductionmentioning

confidence: 99%

Inhibitions by Hydrogen-Occluding Silica Microcluster to Melanogenesis in Human Pigment Cells and Tyrosinase Reaction

Kato

Saitoh²,

Miwa³

2013

j. nanosci. nanotech.

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“…It is a glycosylated derivative of hydroquinone (4-hydroquinone-b-D-glucopyranoside), which is a highly hydrophilic (log p value ¼ À1.49). Some recent studies have catalyzed the synthesis of a-arbutin (4-hydroquinone-a-Dglucopyranoside, molecular weight ¼ 272.25 Da) by hydroquinone and carbohydrates, which can inhibit the activity of tyrosinase enzyme and reduce the formation of melanin while not affecting cell growth or inducing cell toxicity (Sugimoto et al, 2004;Huang et al, 2012). The inhibition of tyrosinase activity was found to be up to 10 times greater for a-arbutin than for b-arbutin (Seo et al, 2012;Liu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Penetration depth, concentration and efficiency of transdermal α-arbutin delivery after ultrasound treatment with albumin-shelled microbubbles in mice

Liao

Wang

et al. 2014

Drug Delivery

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Penetration depth, concentration and efficiency of transdermal α-arbutin delivery after ultrasound treatment with albumin-shelled microbubbles in mice, Drug Delivery, 23:7, 2173-2182, DOI: 10.3109/10717544.2014 AbstractRecently, the feasibility and effects of using microbubbles (MBs) as an ultrasound (US) contrast agent for enhancing the penetration in transdermal delivery in vivo have been demonstrated, but the mechanism and efficiency are unclear. This study demonstrates the penetration depth, concentration and efficiency of transdermal a-arbutin delivery during 4 weeks after US treatment with MBs in mice. Experimental animals were randomly divided into the following four groups (n ¼ 5 animals per group): (1) penetrating a-arbutin alone (C), (2) US combined with penetrating a-arbutin, (3) US combined with MBs and penetrating a-arbutin, and (4) US combined with diluted MBs and penetrating a-arbutin (UBD). The penetration depths in agarose phantoms and pigskin were 47 and 84% greater for group UBD, respectively, than for group C. The in vitro skin penetration by 2% a-arbutin after 3 h was 83% greater in group UBD than in group C. The degree of in vivo skin whitening (quantified as the luminosity index) in group UBD significantly increased by 25% after 1 week, 34% after 2 weeks, and then stabilized after 3 weeks at 37% in C57BL/6J mice over a 4-week experimental period. Our results indicate that combined treatment with optimal US and MBs can increase skin permeability so as to enhance a-arbutin delivery to inhibit melanogenesis without damaging the skin in mice.

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“…Se ha determinado la presencia de fenoles en flores de magnolias, como en M. grandiflora, según investigaciones de Huang et al (2012). Para M. schiedeana no se encontró información sobre los compuestos químicos presentes en flores, por lo que el realizar estudios químicos de esta especie orientará en sus aplicaciones biológicas.…”

Section: Análisis Estadísticosunclassified

Extractos crudos de Magnolia schiedeana Schltdl. para el control de bacterias fitopatógenas

Ramírez-Reyes

Flores‐Estévez

Luna-Rodríguez

et al. 2016

MYB

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ResumenSe evaluó el efecto bactericida de extractos crudos de diferentes tejidos de Magnolia schiedeana sobre las bacterias fitopatógenas Pectobacterium carotovorum y Pseudomonas cichorii. Los tejidos estudiados fueron polifolículo, semilla, corteza (proveniente de especímenes adultos y de especímenes jóvenes), flor y hoja (proveniente de especímenes adultos y de especímenes jóvenes), utilizando como disolventes acetato de etilo y de etanol. Los efectos bactericidas de los extractos fueron comparados con el efecto de un antibiótico comercial (tetraciclina, 5 µg/µl). Los extractos que mostraron inhibición bacteriana fueron, el obtenido con acetato de etilo a partir de tejido floral, el cual no igualó la inhibición del antibiótico comercial sobre P. cichorii ( p < 0.001) y el extracto etanólico floral, que inhibió el crecimiento de P. carotovorum en la misma proporción que el antibiótico (p = 0.079).Palabras clave: bacterias fitopatógenas, fenología, magnolia, Pectobacterium carotovorum, Pseudomonas cichorii. AbstRActBactericidal effect was assessed in crude extracts of different tissues of Magnolia schiedeana about the phytopathogenic bacteria Pectobacterium carotovorum and Pseudomonas cichorii. The tissues studied were polifolicule, seed, bark (obtained from adult specimens and young specimens), flower and leave (from both adult and young specimens as well), using as solvents ethyl acetate and ethanol. The extracts that showed bacterial inhibition were obtained one with ethyl acetate from floral tissue, which did not equaled the inhibiting properties of a commercial antibiotic (tetracycline, 5 µg/µl) on P. cichorii (p < 0.001); and the other was floral ethanol extract that inhibited P. carotovorum in the same proportion as the antibiotic (p = 0.079).Key words: phytopathogenic bacteria, phenology, magnolia, Pectobacterium carotovorum, Pseudomonas cichorii. IntRoduccIónLa seguridad ambiental se ha convertido en una gran preocupación para muchos países debido, entre otros aspectos, al empleo de agroquímicos, los cuales pueden causar daños ambientales severos, tanto al suelo y animales, como a la salud humana, provocando distintos tipos de cánceres, linfomas, asma, dermatitis alérgica, enfermedades respiratorias, malformaciones congénitas, entre otras (Zúñiga et al., 2007;Teng et al., 2010).Aunado a ello, el incremento de multirresistencia en organismos fitopatógenos está impulsando la búsqueda de nuevos agentes biocidas de fuentes alternativas, destacando entre estos a las plantas medicinales (Roy et al., 2010;Vukovic et al., 2007).

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Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract

Cited by 45 publications

References 33 publications

Inhibitions by Hydrogen-Occluding Silica Microcluster to Melanogenesis in Human Pigment Cells and Tyrosinase Reaction

Inhibitions by Hydrogen-Occluding Silica Microcluster to Melanogenesis in Human Pigment Cells and Tyrosinase Reaction

Penetration depth, concentration and efficiency of transdermal α-arbutin delivery after ultrasound treatment with albumin-shelled microbubbles in mice

Extractos crudos de Magnolia schiedeana Schltdl. para el control de bacterias fitopatógenas

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