Inhibition of melanoma cell proliferation by resveratrol is correlated with upregulation of quinone reductase 2 and p53

Hsieh, Tze‐chen; Wang, Zhirong; Hamby, Carl V.; Wu, Joseph M.

doi:10.1016/j.bbrc.2005.06.073

Cited by 72 publications

(52 citation statements)

References 36 publications

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“…The most prominent example is that NQO2 knockout mice are far more prone to develop skin tumors after the topical application of carcinogens (74). More recently, inhibition of melanoma cell growth and clonogenicity was correlated with upregulation of NQO2 and p53 (75). In this context, the parallel inhibition of cell growth induced by melatonin, the natural ligand of NQO2, suggests that expression of NQO2 might be another factor determining the oncostatic effects of melatonin.…”

Section: Discussionmentioning

confidence: 99%

Oncostatic effects of the indole melatonin and expression of its cytosolic and nuclear receptors in cultured human melanoma cell lines

Fischer

Żmijewski²,

Zbytek³

et al. 2006

Int J Oncol

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Abstract.Melatonin has been shown to have oncostatic effects on malignant melanoma in vitro and in vivo. We studied the growth suppressive effects of melatonin over a wide range of concentrations in four melanoma cell lines (SBCE2, WM-98, WM-164 and SKMEL-188) representative for different growth stages and phenotype. Melanoma cells were incubated with melatonin 10 -12 -10 -3 M, and proliferation and clonogenicity was assessed at 12 h and 14 days, respectively. We also determined the expression of cytosolic quinone oxidoreductases NQO1, NQO2 (known as MT3 receptor) and nuclear receptor ROR· by RT-PCR. Melatonin at pharmacological concentrations (10 -3 -10 -7 M) suppressed proliferation in all melanoma cell lines. In SKMEL-188 cells cultured in serum-free media, melatonin at low concentrations (10 -12 -10 -10 M) also slightly attenuated the proliferation. The effects of pharmacological doses of melatonin were confirmed in the clonogenic assay. Expression of NQO1 was detected in all cell lines, whereas NQO2 and nuclear receptor ROR· including its isoform ROR·4 were present only in SBCE2, WM-164 and WM-98. Thus, melatonin differentially suppressed proliferation in melanoma cell lines of different behaviour. The intensity of the oncostatic response to melatonin could be related to the cell-line specific pattern of melatonin cellular receptors and cytosolic binding protein expression.

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Section: Discussionmentioning

confidence: 99%

Oncostatic effects of the indole melatonin and expression of its cytosolic and nuclear receptors in cultured human melanoma cell lines

Fischer

Żmijewski²,

Zbytek³

et al. 2006

Int J Oncol

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“…Control and treated cells were analyzed by flow cytometry, using the procedure and method described previously (5). To determine the full range of changes in DNA content distribution, including hyperdiploid cells, the fluorescent intensity of cells from the RV-treated cultures was additionally measured at lower photomultiplier sensitivity (voltage).…”

Section: Flow Cytometrymentioning

confidence: 99%

“…Whole cell lysates were prepared by freeze/thaw cycles, as previously reported (5,7). Separation of proteins and immunoblot analysis using antibodies against cyclin D1 and D3, cyclin E, cyclin A, cyclin B1, CDKs 2/4/6, CDK1, and p21 WAF1 , and with signal for actin as control for protein loading, were as previously described (5,7).…”

Section: Preparation Of Whole Cell Extracts and Western Blot Analysismentioning

confidence: 99%

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The 2,6-disubstituted purine reversine induces growth arrest and polyploidy in human cancer cells

Hsieh

Traganos²,

Darżynkiewicz³

et al. 2007

Int J Oncol

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Reversine (RV) is the synthetic purine identified from a protein kinase-based screen of purine mimetics and it has been shown to induce muscle myoblast differentiation into progenitor cells that can be further converted into other cell lineages. Since protein kinases play a pivotal role in cell cycle control, we hypothesize that RV might affect the proliferation of cancer cells. Herein we report that RV inhibited growth of cultured human tumor cells, respectively, PC-3, HeLa, CWR22Rv1, and DU-145 cells, and induced accumulation of polyploidal cells with ≥4N DNA content. However, RV was without effect on growth of normal prostate epithelial cells. RV-treated PC-3 cells showed enlarged nuclei and an estimated 100-fold increase in cell size. Moreover, PC-3 cells treated with RV for 2-4 days were accompanied by a marked increase in the expression of p21 WAF1 , a modest elevation in the levels of cyclin D3 and CDK6 and concomitantly, also a substantial reduction in cyclin B and CDK1. These results suggest that RV may induce polyploidy and increase in cell size by up-regulating p21 WAF1 and cyclin D3/CDK6, while simultaneously suppressing the expression of cyclin B and CDK1.

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“…Resveratrol is an atoxic phytoestrogen, which holds great promise as an anti-oxidant, antiinflammatory and/or antitumor agent for various therapeutical indications [9][10][11][12][13][14][15][16][17]. The present review focuses on some of the mechanisms by which resveratrol may reverse or delay the symptoms of cardiovascular aging.…”

Section: Introductionmentioning

confidence: 99%

Vascular Dysfunction in Aging: Potential Effects of Resveratrol, an Anti- Inflammatory Phytoestrogen

Labinskyy¹,

Csiszár²,

Veress³

et al. 2006

CMC

132

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Epidemiological studies demonstrated that even in the absence of other risk factors (e.g. diabetes, hypertension, hyperhomocysteinemia, hypercholesterolemia), advanced age itself significantly increases cardiovascular morbidity by enhancing vascular oxidative stress and inflammation. Because the population in the Western world is rapidly aging, there is a substantial need for pharmacological interventions that delay the functional decline of the cardiovascular system. Resveratrol is an atoxic phytoestrogen found in more than 70 plants including grapevine and berries. Recent data suggest that nutritional intake of resveratrol and other polyphenol compounds may contribute to the "French paradox", the unexpectedly low cardiovascular morbidity in the Mediterranean population. There is increasing evidence that resveratrol exerts multifaceted antioxidant and/or anti-inflammatory effects in various disease models. Importantly, resveratrol was reported to slow aging and increase lifespan in simple organisms and has been suggested as a potential calorie restriction mimetic. Resveratrol has also been reported to activate NAD-dependent histone deacetylases (sirtuins), which may contribute to its anti-aging effects. This review focuses on the role of oxidative stress and inflammation in cardiovascular dysfunction in aging, and on emerging anti-aging therapeutic strategies offered by resveratrol and other polyphenol compounds.

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Inhibition of melanoma cell proliferation by resveratrol is correlated with upregulation of quinone reductase 2 and p53

Cited by 72 publications

References 36 publications

Oncostatic effects of the indole melatonin and expression of its cytosolic and nuclear receptors in cultured human melanoma cell lines

Oncostatic effects of the indole melatonin and expression of its cytosolic and nuclear receptors in cultured human melanoma cell lines

The 2,6-disubstituted purine reversine induces growth arrest and polyploidy in human cancer cells

Vascular Dysfunction in Aging: Potential Effects of Resveratrol, an Anti- Inflammatory Phytoestrogen

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