Inhibition of mesophilic solid-substrate anaerobic digestion by ammonia nitrogen

Poggi‐Varaldo, Héctor M.; Rodrı́guez-Vázquez, Refugio; Fernández-Villagómez, G.; Esparza-Garcı́a, F.

doi:10.1007/s002530050928

Cited by 130 publications

(42 citation statements)

References 25 publications

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“…The above parameters of the materials are comparable to literature data [21]. The concentration of ammonium nitrogen N-NH 4 + which is known to have an inhibitory effect on anaerobic digestion for BM (0.35%), CW (0.32%), as well as for a digested sludge -or the inoculum (2.48%) -does not exceed the critical ammonia N concentration [29]. Buttermilk (273 mg CaCO 3 /dm 3 ) and cheese whey (352 mg CaCO 3 /dm 3 ) in the present study had alkalinities similar to those reported in literature [16].…”

Section: Resultssupporting

confidence: 85%

Treatment of dairy waste by anaerobic co-digestion with sewage sludge

Pilarska

Pilarski

Witaszek

et al. 2016

Ecological Chemistry and Engineering S

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Abstract:The results of anaerobic digestion (AD) of buttermilk (BM) and cheese whey (CW) with a digested sewage sludge as inoculum is described. The substrate/inoculum mixtures were prepared using 10% buttermilk and 15% cheese whey. The essential parameters of the materials were described, including: total solids (TS), volatile solids (VS), pH, conductivity, C/N ratio (the quantitative ratio of organic carbon (C) to nitrogen (N)), alkalinity, chemical oxygen demand (COD). The potential directions of biodegradation of the organic waste types, as used in this study, are also presented. Appropriate chemical reactions illustrate the substrates and products in each phase of anaerobic decomposition of the compounds that are present in buttermilk and cheese whey: lactic acid, lactose, fat, and casein. Moreover, the biogas and biomethane production rates are compared for the substrates used in the experiment. The results have shown that buttermilk in AD generates more biogas

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Section: Resultssupporting

confidence: 85%

Treatment of dairy waste by anaerobic co-digestion with sewage sludge

Pilarska

Pilarski

Witaszek

et al. 2016

Ecological Chemistry and Engineering S

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“…However, during spring 2009 increasing NH 4 -N and NH 3 -N concentrations correlated with increased process instability (Figure 3). The , consisting of 93% propionic acid, which has previously been identified as a common product during NH 3 -N inhibition [30,31]. The level at which NH 3 -N caused inhibition in the process, 450 mg kg −1 , is similar to inhibitory levels reported previously [11,30,32].…”

Section: Nitrogensupporting

confidence: 78%

Biogas Production from Thin Stillage on an Industrial Scale—Experience and Optimisation

Moestedt¹,

Påledal²,

Schnürer

et al. 2013

Energies

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Abstract:With the increasing demand for renewable energy and sustainable waste treatment, biogas production is expanding. Approximately four billion litres of bio-ethanol are produced annually for vehicle fuel in Europe, resulting in the production of large amounts of stillage residues. This stillage is energy-rich and can be used for biogas production, but is a challenging substrate due to its high levels of nitrogen and sulphate. At the full-scale biogas production plant in Norrköping, Sweden (Svensk Biogas i Linköping AB), thin grain stillage is used as a biogas substrate. This paper describes the plant operation and strategies that have been implemented to digest thin stillage successfully. High ammonia concentrations in the digester have resulted in syntrophic acetate oxidation (SAO) becoming the major pathway for acetate degradation. Therefore, a long hydraulic retention time (HRT) (40-60 days) is used to allow the syntrophic acetate-oxidising bacteria time to grow. The high sulphate levels in thin stillage result in high levels of hydrogen sulphide following degradation of protein and the activity of sulphate-reducing bacteria (SRB), the presence of which has been confirmed by quantitative polymerase chain reaction (qPCR) analysis. To optimise biogas production and maintain a stable process, the substrate is diluted with tap water and co-digested with grain residues and glycerine to keep the ammonium nitrogen (NH 4 -N) concentration below 6 g L −1 . Combined addition of iron, hydrochloric acid and cobalt successfully precipitates sulphides, reduces ammonia toxicity and supplies microorganisms with trace element. Mesophilic temperature (38 °C) is employed to further OPEN ACCESSEnergies 2013, 6 5643 avoid ammonia toxicity. Together, these measures and doubling the digester volume have made it possible to increase annual biogas production from 27.7 TJ to 69.1 TJ.

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“…and the hydrogenotrophic methanogens also prosper at high concentrations (3,17,32). The critical total NH 4 ϩ -N concentrations may range between 2.5 and 8.0 g liter Ϫ1 , depending on the supplied substrates (48). For pure culture of Methanosaeta concilii a maximum concentration of less than 1.1 g liter…”

Section: Discussionmentioning

confidence: 99%

Polyphasic Analyses of Methanogenic Archaeal Communities in Agricultural Biogas Plants

Nettmann

Bergmann

Pramschüfer

et al. 2010

Appl Environ Microbiol

156

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Knowledge of the microbial consortia participating in the generation of biogas, especially in methane formation, is still limited. To overcome this limitation, the methanogenic archaeal communities in six full-scale biogas plants supplied with different liquid manures and renewable raw materials as substrates were analyzed by a polyphasic approach. Fluorescence in situ hybridization (FISH) was carried out to quantify the methanogenic Archaea in the reactor samples. In addition, quantitative real-time PCR (Q-PCR) was used to support and complete the FISH analysis. Five of the six biogas reactors were dominated by hydrogenotrophic Methanomicrobiales. The average values were between 60 to 63% of archaeal cell counts (FISH) and 61 to 99% of archaeal 16S rRNA gene copies (Q-PCR). Within this order, Methanoculleus was found to be the predominant genus as determined by amplified rRNA gene restriction analysis. The aceticlastic family Methanosaetaceae was determined to be the dominant methanogenic group in only one biogas reactor, with average values for Q-PCR and FISH between 64% and 72%. Additionally, in three biogas reactors hitherto uncharacterized but potentially methanogenic species were detected. They showed closest accordance with nucleotide sequences of the hitherto unclassified CA-11 (85%) and ARC-I (98%) clusters. These results point to hydrogenotrophic methanogenesis as a predominant pathway for methane synthesis in five of the six analyzed biogas plants. In addition, a correlation between the absence of Methanosaetaceae in the biogas reactors and high concentrations of total ammonia (sum of NH 3 and NH 4 ؉ ) was observed.During the last decade the production of biogas from organic materials and residues has increased continuously in order to reduce the greenhouse gas emission resulting from the use of fossil energy sources. The energy-bearing substance of biogas is methane, which is produced as an end product of microbial anaerobic degradation of organic substrates, such as energy crops like maize, grains, grasses, or beets. Research for optimization of biogas production from renewable materials was initially focused on the evaluation of substrate eligibility and on the development and optimization of technical systems. However, biogas formation primarily depends on the structure and activity of the microbial community (28).The key microorganisms in the biogas formation process are the methane-generating microorganisms (methanogens). The capacity for methanogenesis is limited to members of the domain Archaea and, within this domain, on the phylum Euryarchaeota. With respect to the main metabolic precursors used, methanogens are usually divided into two groups: the aceticlastic methanogens that strictly metabolize acetate and the hydrogenotrophic methanogens that use H 2 or formate as an electron donor and CO 2 as a carbon source for their metabolism. Besides these major groups, certain methanogens are also able to convert methyl groups, methylamines, or methanol to methane (23,40). The substrates for the me...

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Inhibition of mesophilic solid-substrate anaerobic digestion by ammonia nitrogen

Cited by 130 publications

References 25 publications

Treatment of dairy waste by anaerobic co-digestion with sewage sludge

Treatment of dairy waste by anaerobic co-digestion with sewage sludge

Biogas Production from Thin Stillage on an Industrial Scale—Experience and Optimisation

Polyphasic Analyses of Methanogenic Archaeal Communities in Agricultural Biogas Plants

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