Inhibition of microRNA-29c protects the brain in a rat model of prolonged hypothermic circulatory arrest

Abstract

Inhibition of miR-29c attenuates neurologic injuries induced by prolonged deep hypothermia circulatory arrest through a peroxisome proliferator-activated receptor gamma coactivator 1-alpha pathway.

Overexpression of microRNA-30a contributes to the development of aortic dissection by targeting lysyl oxidase

“…Lentivirus vector was used to transfer agomiR and antagomiR in vitro and in vivo. 14,15 Chemically modified agomiR-30a and antagomiR-30a lentivirus gene transfer vectors were constructed by Genechem (Shanghai, China). Empty lentivirus agomiR-vectors or antagomiR-vectors were used as controls.…”

Modulating microRNAs in cardiac surgery patients: Novel therapeutic opportunities?

“…Aortic surgery patients are also at risk of neurological injury by prolonged deep hypothermia circulatory arrest. Inhibition of miR-29c was suggested as protective for the neurological function in a rat study (Wang et al, 2015). Albeit preliminary, these observation hold promise for future exploration (Squiers et al, 2015) as neurological injury is a devastating complication after cardiovascular surgery and few pharmacological agents or interventions are available to effectively protect the brain and spinal cord in the postoperative period (Torres and Ishida, 2015).…”

Inhibition of long noncoding RNA growth arrest–specific 5 attenuates cerebral injury induced by deep hypothermic circulatory arrest in rats

“…Adeno-associated viral vectors of GAS5 siRNA or control vectors were transfected in vivo by means of intracerebroventricular injection 14 days before DHCA, as reported previously. 17 Rats were included in the following protocol only if they had normal neurologic function 3 days after the injection.…”

“…A rat model of cardiopulmonary bypass (CPB) and DHCA was conducted according to the method described previously (Video 1). 17…”